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61.
Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells
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Larissa Bittencourt da Silva Jeppe Nørgaard Poulsen Lars Arendt‐Nielsen Parisa Gazerani 《Journal of cellular and molecular medicine》2015,19(8):1900-1909
This study investigated the presence of cell membrane docking proteins synaptosomal‐associated protein, 25 and 23 kD (SNAP‐25 and SNAP‐23) in satellite glial cells (SGCs) of rat trigeminal ganglion; whether cultured SGCs would release glutamate in a time‐ and calcium‐dependent manner following calcium‐ionophore ionomycin stimulation; and if botulinum neurotoxin type A (BoNTA), in a dose‐dependent manner, could block or decrease vesicular release of glutamate. SGCs were isolated from the trigeminal ganglia (TG) of adult Wistar rats and cultured for 7 days. The presence of SNAPs in TG sections and isolated SGCs were investigated using immunohistochemistry and immunocytochemistry, respectively. SGCs were stimulated with ionomycin (5 μM for 4, 8, 12 and 30 min.) to release glutamate. SGCs were then pre‐incubated with BoNTA (24 hrs with 0.1, 1, 10 and 100 pM) to investigate if BoNTA could potentially block ionomycin‐stimulated glutamate release. Glutamate concentrations were measured by ELISA. SNAP‐25 and SNAP‐23 were present in SGCs in TG sections and in cultured SGCs. Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001). BoNTA (100 pM) significantly decreased glutamate release (P < 0.01). Results from this study demonstrated that SGCs, when stimulated with ionomycin, released glutamate that was inhibited by BoNTA, possibly through cleavage of SNAP‐25 and/or SNAP‐23. These novel findings demonstrate the existence of vesicular glutamate release from SGCs, which could potentially play a role in the trigeminal sensory transmission. In addition, interaction of BoNTA with non‐neuronal cells at the level of TG suggests a potential analgesic mechanism of action of BoNTA. 相似文献
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Ghabaee M Bayati A Amri Saroukolaei S Sahraian MA Sanaati MH Karimi P Houshmand M Sadeghian H Hashemi Chelavi L 《Cellular and molecular neurobiology》2009,29(1):109-114
Multiple sclerosis (MS) is prototype of inflammatory demyelinating disease of the central nervous system .The etiology of
MS remains unclear, but according to current data the disease develops in genetically susceptible individuals and may require
additional environmental triggers. The human leukocyte antigen (HLA) class II alleles (DRB1*1501, DQA1*0102, DQB1*0602) may
have the strongest genetic effect in MS. In this study, the role of these alleles were investigated in 183 Iranian patients
with multiple sclerosis and compared with 100 healthy individuals. HLA typing for DRB1*1501, DQA1*0102, DQB1*0602 was performed
by polymerase chain reaction (PCR) amplification with sequence-specific primers (PCR-SSP) method. The results show that, HLA
DR B1*1501 was significantly more frequent among MS patients (46% vs. 20%, PV = 0.0006) but DQA1*0102 haplotype was negatively
associated with MS (30% vs. 50%, PV = 0.0049) and no significant association was found with DQB1*0602 and MS patients in comparison
with control group (24% and 30%, PV = 0.43). No significant correlation was observed among these alleles with sex, type of
disease; initial symptoms, expanded disability status scale (EDSS), as well as age at onset and familial MS. This study therefore
indicates that there is no association of above HLA haplotypes with clinical presentation, disease duration, and disability
in Iranian patients with MS which is in line with other previous studies in different ethnic groups. 相似文献
63.
Runnels JM Zamiri P Spencer JA Veilleux I Wei X Bogdanov A Lin CP 《Molecular imaging》2006,5(1):31-40
Molecular expression on the vascular endothelium is critical in regulating the interaction of circulating cells with the blood vessel wall. Leukocytes as well as circulating cancer cells gain entry into tissue by interacting with adhesion molecules on the endothelial cells (EC). Molecular targets on the EC are increasingly being explored for tissue-specific delivery of therapeutic and imaging agents. Here we use in vivo immunofluorescence microscopy to visualize the endothelial molecular expression in the vasculature of live animals. High-resolution images are obtained by optical sectioning through the intact skin using in vivo confocal and multiphoton microscopy after in situ labeling of EC surface markers with fluorescent antibodies. Other vascular beds such as the bone marrow and ocular blood vessels can be imaged with little or no tissue manipulation. Live imaging is particularly useful for following the dynamic expression of inducible molecules such as E-selectin during an inflammatory response. 相似文献
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Samaneh Arman Marzieh Hadavi Azadeh Rezvani-Noghani Anashid Bakhtparvar Melika Fotouhi Ali Farhang Parisa Mokaberi Reza Taheri Jamshidkhan Chamani 《Luminescence》2024,39(1):e4634
In this study, cellulose nanocrystals (CNCs) were synthesized from celery stalks to be used as the platform for quercetin delivery. Additionally, CNCs and CNCs–quercetin were characterized using the results of scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and zeta potential, while their interactions with human holo-transferrin (HTF) were also investigated. We examined their interaction under physiological conditions through the exertion of fluorescence, resonance light scattering, synchronized fluorescence spectroscopy, circular dichroism, three-dimensional fluorescence spectroscopy, and fluorescence resonance energy transfer techniques. The data from SEM and TEM exhibited the spherical shape of CNCs and CNCs–quercetin and also, a decrease was detected in the size of quercetin-loaded CNCs from 676 to 473 nm that indicated the intensified water solubility of quercetin. The success of cellulose acid hydrolysis was confirmed based on the XRD results. Apparently, the crystalline index of CNCs–quercetin was reduced by the interaction of CNCs with quercetin, which also resulted in the appearance of functional groups, as shown by FTIR. The interaction of CNCs–quercetin with HTF was also demonstrated by the induced quenching in the intensity of HTF fluorescence emission and Stern–Volmer data represent the occurrence of static quenching. Overall, the effectiveness of CNCs as quercetin vehicles suggests its potential suitability for dietary supplements and pharmaceutical products. 相似文献
66.
Zarghi A Tabatabai SA Faizi M Ahadian A Navabi P Zanganeh V Shafiee A 《Bioorganic & medicinal chemistry letters》2005,15(7):1863-1865
A series of new 2-substituted-5-(2-benzyloxyphenyl)-1,3,4-oxadiazoles have been synthesized and evaluated as anticonvulsant agents. Compound 4b shows considerable anticonvulsant activity both in PTZ and MES models. It seems this effect is mediated through benzodiazepine receptors mechanism. 相似文献
67.
Ho V Momtaz P Didas C Wadleigh M Richardson P 《Reviews in clinical and experimental hematology》2004,8(1):E3
Hepatic veno-occlusive disease (VOD) is one of the most important complications following hematopoietic stem cell transplantation (SCT) and is associated with a very high mortality when severe. This review addresses the pathogenesis and clinical features of VOD and outlines the role on endothelial cell injury and risk factors. The current status of research for both treatment and prevention are discussed. 相似文献
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Parisa ZiaSarabi Saba Sorayayi AmirReza Hesari Faezeh Ghasemi 《Journal of cellular biochemistry》2019,120(8):12376-12381
Gastric cancer is one of the most common malignancies in the world and is considered as the most lethal gastrointestinal cancer. microRNAs (miRNAs) can be very important in detecting a disease at an early stage. The aim of this study was to investigate the microRNA-17 (miR-17), miR-25, and miR-133b in the serum of gastric cancer subjects. Serum samples were obtained from 120 gastric cancers and 102 healthy subjects. We evaluated expression levels of miR-17, miR-25 and miR-133b by quantitative real-time polymerase chain reaction. Our results showed that in the patients with gastric cancer, the expression level of miR-17 and miR-25 were significantly increased compared with the control group (P < 0.5), while the expression level of miR-133b was significantly decreased in patient groups compared with control cases (P < 0.5). It seems that expression of miRNAs in Iranian patients with gastric cancer is similar to other patients in other populations. These findings suggested that miR-17, miR-25 and miR-133b could be introduced as potential diagnostic candidates for the detection in gastric cancer patients in the early stage. 相似文献
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Parisa Amiri Tina Deihim Reza Taherian Mehrdad Karimi Safoora Gharibzadeh Mohammad Asghari-Jafarabadi Niloofar Shiva Fereidoun Azizi 《PloS one》2015,10(12)