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31.
Mohammed Akrim Marc Bally Genevive Ball Jan Tommassen Henk Teerink Alain Filloux Andre Lazdunski 《Molecular microbiology》1993,10(2):431-443
In Pseudomonas aeruginosa, several exoproteins synthesized with a signal sequence (elastase, lipase, phospholipases, alkaline phosphatase and exotoxin A) are secreted by a two-step mechanism. They first cross the inner membrane in a signal sequence-dependent way, and are further translocated across the outer membrane in a second step requiring secretion functions encoded by several xcp genes. Ten xcp genes have already been characterized (Bally et al., 1992a). In this study, two additional xcp genes, xcpP and xcpQ, are described. They are located in the 40 min region of the chromosome where they probably define an operon, divergent from the xcpR–Z operon previously characterized in this region. These two genes encode two proteins, XcpP and XcpQ, similar to PulC and PulD of the pul system of Klebsiella oxytoca. Moreover, the two divergent operons share a common regulation which is growth-phase dependent. 相似文献
32.
Colonies of the African stink ant Paltothyreus tarsatuslocated in the forest have nests with shorter horizontal galleries and a smaller total foraging surface than colonies located in open areas. Each solitary worker specializes on the same central or peripheral hunting zone but she does not specialize on a particular sector during group-retrieving. The search for prey is characterized by a wandering walk with spatial parameters varying in two ways. Capture of a termite releases a path characterized by sinuosity and a decrease in speed of movement. In contrast, a failure in the course of an attempted capture releases an increase in both sinuosity and speed of movement corresponding to a socalled reserve behavior. Each worker shortens her retrieving trip in comparison with her search trip and the straightness of the homing paths depends on the size and shape of the prey. Our data show that behavioral flexibility at the individual level in P. tarsatusis important in determining spatial foraging strategy at the colony level. 相似文献
33.
The distribution of NO3? reduction between roots and shoots was studied in hydro-ponically-grown peach-tree seedlings (Prunus persica L.) during recovery from N starvation. Uptake, translocation and reduction of NO3?, together with transport through xylem and phloem of the newly reduced N were estimated, using 15N labellings, in intact plants supplied for 90 h with 0.5 mM NH4+ and 0.5, 1.5 or 10 mM NO3?. Xylem transport of NO3? was further investigated by xylem sap analysis in a similar experiment. The roots were the main site of NO3? reduction at all 3 levels of NO3? nutrition. However, the contribution of the shoots to the whole plant NO3? reduction increased with increasing external NO3? availability. This contribution was estimated to be 20, 23 and 42% of the total assimilation at 0.5, 1.5 and 10 mM NO3?, respectively. Both 15N results and xylem sap analysis confirmed that this trend was due to an enhancement of NO3? translocation from roots to shoots. It is proposed that the lack of NO3? export to the shoots at low NO3? uptake rate resulted from a competition between NO3? reduction in the root epidermis/cortex and NO3? diffusion to the stele. On the other hand, net xylem transport of newly reduced N was very efficient since ca 70% of the amino acids synthesized in the roots were translocated to the shoots, regardless of the level of NO3? nutrition. This net xylem transport by far exceeded the net downward phloem transport of the reduced N assimilated in shoots. As a consequence, the reduced N resulting from NO3? assimilation, principally occurring in the roots, was mainly incorporated in the shoots. 相似文献
34.
The territorial African weaver ant Oecophylla longinoda forages in a 3-dimensional system when attending honeydew secreted by coccid colonies. The orienting strategy allowing workers to reach a static food resource was studied in laboratory conditions. Contrary to other studies devoted to territoriality in O. longinoda, our experiments show that, when moving off the nest, territorial faecal marking by the major workers is not randomly placed all over the home-range area. The chemical trail leading from the nest to a given food site is reinforced by faecal materials both on the horizontal and the vertical planes. In addition to visual cues and chemical trails laid by the major workers (Holldobler & Wilson 1978), foragers use the territorial marking both to localize the food site and to come back to the nest. Thus, anal-drop deposition in O. longinoda also has a clear dual-purpose function: territorial and orientationai marking. The ecological value of such a discrete Tom Thumb's orienting strategy enables workers to quickly reach a food location even after more than 5 months of inactivity on this given site. The persistent marked trail also has an intercolonial effect. This could allow the keeping of exploitation of a definite food site by alien colonies of the same species by reducing time costs for new exploring phases or for learning new spatial cues when foraging on an unknown environment. 相似文献
35.
Machhour Ghazali Michel Philippe Alain Deguercy Pierre Gounon Jean-Marc Gallo Joseph Schrevel 《Biology of the cell / under the auspices of the European Cell Biology Organization》1989,67(2):173-184
In Gregarina blaberae a Mr = 47 000 and a Mr = 260–240 000 doublet polypeptides reacted in immunoblotting: i) with a polyclonal monospecific rabbit antibody to frog muscular actin, a monoclonal anti-actin antibody against chicken gizzard; and ii) with polyclonal and monoclonal antibodies to human erythrocyte β-spectrin, respectively. The Mr = 47 000 actin-like protein is associated with the ghost and a contractille cytoplasmic extract. The presence of an actin-like protein in Gregarina and Lecudina and its cellular distribution in the cortex indicated that the gliding movement might involve an actin-myosin system in contrast to previous studies. Immunofluorescence showed clear differences between the anterior part of Gregarina and Lecudina which illustrated the high cell polarity of these protozoa. The Mr = 260–240 000 doublet was detected in SDS-PAGE from G. blaberae trophozoite ghosts but not in the cytoplasmic extracts or in extracts from sexual stages, indicating that the presence of these spectrin-like proteins is stage-dependent. Visualization of the Mr = 260–240 000 by immunofluorescence showed clear species differences, with rings arranged perpendicular to the longitudinal narrow folds of G. blaberae, with longitudinal lines underlying the folds of L. pellucida and with lines separating the large folds of Selenidium pendula. The cellular distribution is consistent with a stabilizer function of the spectrin-like proteins in the scaffolding of the cortex of gregarines according to the high diversity of the cell-shape and the cell motility systems in gregarines. The presence of spectrin-like proteins in protozoa and particularly in parasites from primitive arthropods indicated that ancestral spectrin genes could the Mr = 260–240 000 form. 相似文献
36.
Anne-Sophie Yribarren Daniel Thomas Alain Friboulet Bérangère Avalle 《European journal of biochemistry》2003,270(13):2789-2795
A library of random peptide sequences was used to select peptides that inhibit an anti-idiotypic catalytic Ig, immunoglobulin (IgG) 9G4H9, with a beta-lactamase-like activity. This library displays cyclic heptapeptides on the surface of bacteriophages and represents a collection of up to 4.5 x 109 peptides. The first selection step aimed at enriching the library in species that bind to the whole Ig molecule. The second step was to discriminate peptides that bind to part of the molecule other than the active site. Selected peptides were then screened by surface plasmon resonance analysis. Those displaying measurable Kd values were assayed for their ability to inhibit the catalytic Ig. 相似文献
37.
G. Chetrite J. Paris J. Botella J. R. Pasqualini 《The Journal of steroid biochemistry and molecular biology》1996,58(5-6):525-531
It is well recognized that estradiol (E2) is one of the most important hormones supporting the growth and evolution of breast cancer. Consequently, to block this hormone before it enters the cancer cell or in the cell itself, has been one of the main targets in recent years. In the present study we explored the effect of the progestin, nomegestrol acetate, on the estrone sulfatase and 17β-hydroxy-steroid dehydrogenase (17β-HSD) activities of MCF-7 and T-47D human breast cancer cells. Using physiological doses of estrone sulfate (E1S: 5 × 10−9 M), nomegestrol acetate blocked very significantly the conversion of E1S to E2. In the MCF-7 cells, using concentrations of 5 × 10−6 M and 5 × 10−5 M of nomegestrol acetate, the decrease of E1S to E2 was, respectively, −43% and −77%. The values were, respectively, −60% and −71% for the T-47D cells. Using E1S at 2 × 10−6 M and nomegestrol acetate at 10−5 M, a direct inhibitory effect on the enzyme of −36% and −18% was obtained with the cell homogenate of the MCF-7 and T-47D cells, respectively. In another series of studies, it was observed that after 24 h incubation of a physiological concentration of estrone (E1: 5 × 10−9 M) this estrogen is converted in a great proportion to E2. Nomegestrol acetate inhibits this transformation by −35% and −85% at 5 × 10−7 M and 5 × 10−5 M, respectively in T-47D cells; whereas in the MCF-7 cells the inhibitory effect is only significant, −48%, at 5 × 10−5 M concentration of nomegestrol acetate. It is concluded that nomegestrol acetate in the hormone-dependent MCF-7 and T-47D breast cancer cells significantly inhibits the estrone sulfatase and 17β-HSD activities which converts E1S to the biologically active estrogen estradiol. This inhibition provoked by this progestin on the enzymes involved in the biosynthesis of E2 can open new clinical possibilities in breast cancer therapy. 相似文献
38.
39.
Delhon Patricia; Gojon Alain; Tillard Pascal; Passama Lucien 《Journal of experimental botany》1995,46(10):1585-1594
The effect of light on NO3 utilization was investigatedin non-nodulated soybean (Clycine max L. Merr., cv. Kingsoy)plants during a 14/10 h light/dark period at a constant temperatureof 26C. A 3050% decrease of net NO3 uptake ratewas observed 26 h after the lights were turned off. Thiswas specifically due to an inhibition of NO3 influx asmeasured by 15N incorporation during 5 min. The absolute valuesof NO3 efflux depended on whether the labelling protocolinvolved manipulation of the plants or not, but were not affectedby illumination of the shoots. Darkness had an even more markedeffect in lowering the reduction of 15NO3 in both rootsand shoots, as well as xylem transport of 15NO3 and reduced15N. Concurrently with this slowing down of transport and metabolicprocesses, accumulations of NO3 and Asn were significantlystimulated in roots during the dark period. These data are discussedin view of the hypothesis that darkness adversely affects NO3uptake through specific feedback control, in response to alterationsin the later steps of N utilization which are more directlydependent on light. Key words: Glycine max, light/dark cycles, nitrate uptake, nitrate reduction 相似文献
40.
François-Yves Dupradeau Guillaume Le Flem Jean-Michel Wieruszeski Manuel Dauchez Alain Alix Véronique Larreta-Garde Jean-Pierre Monti 《Letters in Peptide Science》1997,4(4-6):489-495
H-NMR studies of the bovine insulin S-sulfonatedB-chain are reported in H2O/D2O (9/1) and inglycerol-d5 (5 M) using two-dimensional NMRspectroscopy. The first results show that the oxidizedinsulin B-chain secondary structure differs from thatof native insulin by a loss of the -helixbetween the two disulfide bridges and that theglycerol favours the structuring of the peptide. 相似文献