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21.
Phenylarsine oxide induces the cyclosporin A-sensitive membrane permeability transition in rat liver mitochondria 总被引:2,自引:0,他引:2
Ewa Lenartowicz Paolo Bernardi Giovanni Felice Azzone 《Journal of bioenergetics and biomembranes》1991,23(4):679-688
This paper reports an investigation on the effects of the hydrophobic, bifunctional SH group reagent phenylarsine oxide (PhAsO) on mitochondrial membrane permeability. We show that PhAsO is a potent inducer of the mitochondrial permeability transition in a process which is sensitive to both the oxygen radical scavanger BHT and to cyclosporin A. The PhAsO-induced permeability transition is stimulated by Ca2+ but takes place also in the presence of EGTA in a process that maintains its sensitivity to BHT and cyclosporin A. Our findings suggest that, at variance from other known inducers of the permeability transition, PhAsO reacts directly with functional SH groups that are inaccessible to hydrophilic reagents in the absence of Ca2+. 相似文献
22.
The rheological properties of aqueous solutions of hydroxyethyl guar gum, a synthetic derivative of guar gum, have been studied under continuous and oscillatory shear flow conditions. Data obtained from both experimental techniques were satisfactorily fitted according to Cross-type models. The effect of polymer concentration, molecular weight and temperature on the rheological behavior of hydroxyethyl guar gum systems have been investigated and discussed in terms of rheological parameters like the zero-shear viscosity η0 and the characteristic times λ and λ′. 相似文献
23.
Jean-Marc Gilli Paolo Mariani Franco Rustichelli Bin Yang Hervé Delacroix 《Molecular Engineering》1992,2(2):177-188
We recently showed that a side-chain industrial co-oligosiloxane presents a quenchable enlarged blue phase behaviour at the cholesteric-isotropic phase transition. In this paper, we present the results of a structural study based on X-ray diffraction, differential scanning calorimetry and optical measurements. In particular, the smectic A organisation is demonstrated in the lower temperature domain, which was hitherto understood as a cholesteric phase. A structural model for this phase is proposed on the basis of the analysis of the anisotropic scattering of stretched fibers. Our results also suggest that the observed glass transition is indeed a rather complex phenomenon, which seems to involve not only the freezing of the main chains, but also smectic correlations at the side-chain level. Moreover, the calorimetric study indicates that, notwithstanding the conservation of the processed film's optical properties, low kinetic reorganisations occur at room temperature. 相似文献
24.
Genetic determinants of glutamine synthetase inDrosophila melanogaster: A gene for glutamine synthetase I resides in the 21B3-6 region 总被引:1,自引:0,他引:1
Corrado Caggese Ruggiero Caizzi Maria Pia Bozzetti Paolo Barsanti Ferruccio Ritossa 《Biochemical genetics》1988,26(9-10):571-584
Recombinational and deletion mapping of electrophoretic variants of the glutamine synthetase I isozyme (GSI) in Drosophila melanogaster locates the gene in the 21B region on the second chromosome. We have conducted a genetic analysis of the region extending cytologically from 21A to 21B4-6. Recessive lethal mutations were generated by ethyl methanesulfonate (EMS) and ethyl nitrosourea (ENU) mutagenesis and by hybrid dysgenesis (HD). These lethals fall into seven functional groups, which were partially ordered by complementation with cytologically defined deficiencies of this region generated by hybrid dysgenesis. Two of the EMS- and two of the ENU-induced lethals fulfill biochemical criteria expected for null alleles of the GSI gene. 相似文献
25.
Milzani Aldo; Carrera Paolo; Bergna Giovanni; Colombo Roberto 《Bioinformatics (Oxford, England)》1986,2(1):19-22
A program in BASIC is described which allows accurate quantificationof some numerical parameters that can be objectively correlatedto biological indexes in sigmoid biological events. Attentionwas focused on the polymerization process of actin (a muscleprotein with a mol. wt of 42 000 daltons) studied as the variationin the OD360 index with time. The experimental points, if plotted,can be well approximated by a rational function of the typeOD360 = f(t), which passes through the origin and can be representedgraphically by a sigmoid curve. The program was very helpfulin comparing the experimental curves and in analysing significantparameters, such as maximum velocity and asymptote, that characterizethese curves and whose interpretation would otherwise be purelysubjective.
Received on July 11, 1985; accepted on January 13, 1986 相似文献
26.
Estimation of the size of freshwater ciliate populations by a sub-sampling technique 总被引:4,自引:4,他引:0
Paolo Madoni 《Hydrobiologia》1984,111(3):201-206
A technique is described for the estimation of the size of ciliate populations based on replicated counts. Sample drops were
taken by automatic pipettes of different volume from activated sludge and from a small oligotrophic stream. Specific aims
were: 1) the estimation of the number of species; 2) the estimation of the number of individual in each species; 3) the selection
of a suitable sub-sample size and number of replicates. For each volume the cumulative increase in species taken in successive
sub-samples was determined. For each species the minimum permissible sub-sample volume and the number of replicates required
for an allowable error of 10% and 25% were determined. Lastly, the relationship between the mean number of individuals counted
for each species and the relative coefficient of variation was visualized. 相似文献
27.
Dion tomasellii sp. nov. occurs with an interrupted distribution from Guerrero to central Sonora. It is characterized by falcate to subfalcate leaflets. The populations from Sonora and northern Sinaloa are segregated asD. tomasellii var.sonorense on the basis of their narrower and glaucous leaflets. 相似文献
28.
Giorgio M. Hanozet Barbara Giordana Paolo Parenti Andrea Guerritore 《The Journal of membrane biology》1984,81(3):233-240
Summary In brush border membrane vesicles from the midgut ofPhilosamia cynthia larvae (Lepidoptera) thel- andd-alanine uptake is dependent on a potassium gradient and on transmembrane electrical potential difference. Each isomer inhibits the uptake of the other form: inhibition ofl-alanine uptake byd-alanine is competitive, whereas inhibition ofd-alanine uptake byl-alanine is noncompetitive. Transstimulation experiments as well as the different pattern of specificity to cations suggest the existence of two transport systems. Kinetic parameters for the two transporters have been calculated both when Kout>Kin and Kout=Kin.d-alanine is actively transported also by the whole midgut, but it is not metabolized by the intestinal tissue. 相似文献
29.
The isomerization of poly-L -proline in different solvents has been studied by NMR spectroscopy. Different resonance signals for the CHα protons have been obtained for the two different helical conformations of thus compound, namely form I and form II. 相似文献
30.