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31.
N V Kuleva I E Krasovskaia S A Kudinov N S Panteleeva 《Biokhimii?a (Moscow, Russia)》1977,42(6):1052-1055
Mg2+-Dependent, Ca2+-activated adenosine triphosphatase (E. C. 3.6.1.4) of synaptosomal plasmatic membrane from cow brain catalyses isotopic exchange of oxygen atoms: KH2P18O 4 in equilibrium H2O, the degree of exchange depending on Ca2+ concentration. The 18O-exchange catalysis suggests that the enzyme under consideration acts as a transport ATPase. 相似文献
32.
Sorokin DY Zacharova EE Pimenov NV Tourova TP Panteleeva AN Muyzer G 《FEMS microbiology ecology》2012,79(2):445-453
The activity and culturable diversity of sulfidogens were investigated in anoxic sediments of four hypersaline lakes with pH 7.6-8.2 in the Kulunda Steppe (Altai, Russia). Sulfate reduction rates were low, varying from 0.1 to 6.0 nmol HS(-) /(cm(3) h) with a maximum in the top 10 cm layer. Potential sulfidogenic rates with thiosulfate and sulfur as the e-acceptors were higher than with sulfate and were stimulated by formate, lactate, and acetate. Sulfidogenesis was optimal at salt concentrations below 2 M NaCl. Cultivation at 2 M NaCl resulted in the isolation of several strains of moderately halophilic SRB, but no growth of SRB was observed at 4 M NaCl. At lithotrophic conditions (i.e., with formate or H(2) as e-donors), several closely related alkalitolerant strains belonging to the genus Desulfonatronovibrio were isolated. Enrichments at heterotrophic conditions with lactate, propionate, acetate, or butyrate using sulfate or thiosulfate as e-acceptors yielded isolates related to Desulfosalsimonas propionicica, Desulfohalobium utahense, and Desulfocella halophila. Sulfur-reducing enrichments at 2 M NaCl with ethanol produced a member of the genus Halanaerobium, while enrichments at 4 M NaCl with acetate were dominated by archaea, demonstrating for the first time such type of catabolism in haloarchaea. 相似文献
33.
Anita Kaliszewicz Ninel Panteleeva Izabella Olejniczak Pawe? Boniecki Marek Sawicki 《Polar Biology》2012,35(12):1911-1919
Primarily for intertidal species, internal brooding has been reported as a frequently used reproductive strategy of sea anemones. While brooding reduces dispersal, this strategy seems to be beneficial in a harsh environment, such as the intertidal zone. We focused on the species composition, habitat preference and spatial structure of the internally brooding sea anemones, Aulactinia stella and Urticina crassicornis, which both form aggregations in the intertidal zone of the Barents Sea. We analyzed the color patterns of polyps within aggregations and the distance between polyps at different ages. Juveniles settled near the individual parent that released them. The distance between the U. crassicornis parent individual and its juveniles increased with increasing size of the juveniles. This tendency leads to a spatial structure based on the aggregations comprising one or two reproducing individuals that are surrounded by small, identically colored anemones. Our findings revealed that monochromatic aggregations dominated, which suggests that U. crassicornis similar to A. stella may reproduce asexually. A. stella appeared to be more resistant to wave action and more able to colonize the sandy ground compared to U. crassicornis, which prefers large boulders providing shelter, avoids sites exposed to strong wave action and often occurs in dense aggregations in the habitat patches favorable for survival and reproduction. It seems that internal brooding allows for the successful colonization of harsh and heterogeneous habitats, such as the intertidal zone, by counteracting the washout by waves and tidal action. 相似文献
34.
35.
I. A. Maksimova A. M. Glushakova A. V. Kachalkin I. Yu. Chernov S. N. Panteleeva Zh. I. Reznikova 《Microbiology》2016,85(1):124-129
Yeast abundance and species diversity in the colonies of Formica aquilonia ants in birch–pine grass forest near Novosibirsk, Russia, were studied. The average yeast number in the anthill material was 103–104 CFU/g, reaching 105 CFU/g in the hatching chambers. Typical litter species (Trichosporon moniliiforme and Cystofilobasidium capitatum) were predominant in soil and litter around the anthills. Apart from these species, ascomycete species of the family Debaryomycetaceae, Debaryomyces hansenii, and Schwanniomyces vanrijiae were predominant in the anthill material. Yeast population of the ant’ bodies consisted exclusively of the members of the last two species. Thus, highly specific yeast communities formed in the colonies of Formica aquilonia ants differ from the communities of surrounding soil. These differences are caused by environment-forming activity of the ants. 相似文献
36.
37.
E. I. Kompantseva J. F. Imhoff B. Thiemann E. E. Panteleeva V. N. Akimov 《Microbiology》2007,76(5):541-551
The fatty acid composition (FAC) of 43 strains of purple nonsulfur bacteria belonging to six genera—Rubrivivax, Rhodopseudomonas, Rhodoplanes, Blastochloris, Rhodobium, and Rhodomicrobium—was studied by capillary gas chromatography. The cultures were grown on standard medium under standard conditions. Automatic identification of the fatty acid methyl esters and statistical processing of the results were performed by the computerized Microbial Identification System (MIS). Significant differences between the FACs of different genera, species, and, sometimes, strains were revealed. 16S rRNA genes of some of the new isolates, primarily those having a specific FAC, were sequenced. The taxonomic status of a number of the strains in question was determined using the FAC characteristics as one of the criteria. It was shown that the FAC characteristics may be used both for affiliating isolates to known species and for revealing new taxa. 相似文献
38.
V. N. Anisimov L. E. Bakeeva P. A. Egormin O. F. Filenko E. F. Isakova V. N. Manskikh V. M. Mikhelson A. A. Panteleeva E. G. Pasyukova D. I. Pilipenko T. S. Piskunova I. G. Popovich N. V. Roshchina O. Yu. Rybina V. B. Saprunova T. A. Samoylova A. V. Semenchenko M. V. Skulachev I. M. Spivak E. A. Tsybul’ko M. L. Tyndyk M. Yu. Vyssokikh M. N. Yurova M. A. Zabezhinsky V. P. Skulachev 《Biochemistry. Biokhimii?a》2008,73(12):1329-1342
Very low (nano- and subnanomolar) concentrations of 10-(6′-plastoquinonyl) decyltriphenylphosphonium (SkQ1) were found to
prolong lifespan of a fungus (Podospora anserina), a crustacean (Ceriodaphnia affinis), an insect (Drosophila melanogaster), and a mammal (mouse). In the latter case, median lifespan is doubled if animals live in a non-sterile vivarium. The lifespan
increase is accompanied by rectangularization of the survival curves (an increase in survival is much larger at early than
at late ages) and disappearance of typical traits of senescence or retardation of their development. Data summarized here
and in the preceding papers of this series suggest that mitochondria-targeted antioxidant SkQ1 is competent in slowing down
execution of an aging program responsible for development of age-related senescence.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users.
Published in Russian in Biokhimiya, 2008, Vol. 73, No. 12, pp. 1655–1670. 相似文献
39.
Słonina D Spekl K Panteleeva A Brankovic K Hoinkis C Dörr W 《Radiation and environmental biophysics》2003,42(1):55-61
A relative biological effectiveness (RBE) not much larger than unity is usually assumed for soft x-rays (up to approximately 50 keV) that are applied in diagnostic radiology such as mammography, in conventional radiotherapy and in novel radiotherapy approaches such as x-ray phototherapy. On the other hand, there have been recent claims of an RBE of more than 3 for mammography and respective conventional x-rays. Detailed data on the RBE of soft x-rays, however, are scarce. The aim of the present study was to determine the effect of low-energy x-rays on chromosomal damage in vitro, in terms of micronucleus induction. Experiments were performed with 25 kV x-rays and a 200 kV x-ray reference source. The studies were carried out on primary human epidermal keratinocytes (HEKn), human fibroblasts (HFIB) and NIH/3T3 mouse fibroblasts. Micronucleus (MN) induction was assayed after in vitro irradiation with doses ranging from 1 to 5.2 Gy. Compared to the effect of 200 kV x-rays, 25 kV x-rays resulted in moderately increased chromosomal damage in all cell lines studied. This increase was observed for the percentage of binucleated (BN) cells with micronuclei as well as for the number of micronuclei per BN cell. Moreover, the increased number of micronuclei per micronucleated BN cell in human keratinocytes and 3T3 mouse fibroblasts suggests that soft x-rays induce a different quality of damage. For all cell lines studied the analysis of micronucleus induction by 25 kV soft x-rays compared to 200 kV x-rays resulted in an RBE value of about 1.3. This indicates a somewhat enhanced potential of soft x-rays for induction of genetic effects. 相似文献
40.
D. Y. Sorokin T. P. Tourova A. N. Panteleeva E. N. Kaparullina G. Muyzer 《Extremophiles : life under extreme conditions》2012,16(2):307-315
Anaerobic enrichments at pH 10, with pectin and polygalacturonates as substrates and inoculated with samples of sediments
of hypersaline soda lakes from the Kulunda Steppe (Altai, Russia) demonstrated the potential for microbial pectin degradation
up to soda-saturating conditions. The enrichments resulted in the isolation of six strains of obligately anaerobic fermentative
bacteria, which represented a novel deep lineage within the order Clostridiales loosely associated with the family Lachnospiraceae. The isolates were rod-shaped and formed terminal round endospores. One of the striking features of the novel group is a
very narrow substrate spectrum for growth, restricted to galacturonic acid and its polymers (e.g. pectin). Acetate and formate
were the final fermentation products. Growth was possible in a pH range from 8 to 10.5, with an optimum at pH 9.5–10, and
in a salinity range from 0.2 to 3.5 M Na+. On the basis of unique phenotypic properties and distinct phylogeny, the pectinolytic isolates are proposed to be assigned
to a new genus Natranaerovirga with two species N. hydrolytica (APP2T=DSM24176T=UNIQEM U806T) and N. pectinivora (AP3T=DSM24629T=UNIQEM U805T). 相似文献