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61.
Rajasekar A Babu TG Pandian ST Maruthamuthu S Palaniswamy N Rajendran A 《Journal of industrial microbiology & biotechnology》2007,34(9):589-598
A facultative anaerobic species Serratia marcescens ACE2 isolated from the corrosion products of diesel transporting pipeline in North West, India was identified by 16S rDNA
sequence analysis. The role of Serratia marcesens ACE2 on biodegradation of diesel and its influence on the corrosion of API 5LX steel has been elucidated. The degrading strain
ACE2 is involved in the process of corrosion of steel API 5LX and also utilizes the diesel as an organic source. The quantitative
biodegradation efficiency (BE) of diesel was 58%, calculated by gas-chromatography–mass spectrum analysis. On the basis of
gas-chromatography–mass spectrum (GC–MS), Fourier Transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD), the
involvement of Serratia marcescens on degradation and corrosion has been investigated. This basic study will be useful for the development of new approaches
for detection, monitoring and control of microbial corrosion. 相似文献
62.
U. K. Sarkar A. K. Pathak R. K. Sinha K. Sivakumar A. K. Pandian A. Pandey V. K. Dubey W. S. Lakra 《Reviews in Fish Biology and Fisheries》2012,22(1):251-272
The river Ganges is the largest river in India and the fifth longest in the world. Although, many studies on fish ecology
and systematic have been conducted largely to improve fisheries but fish diversity and their distribution pattern from conservation
point of view have never been adequately addressed in the Ganges. In this connection, current distribution and abundance of
freshwater fishes of river Ganges was studied and assessed from April 2007 to March 2009. We documented and described 143
freshwater fish species in the all stretches of the river which is higher than what was reported earlier. Some species were
observed with shift in their distribution ranges. First time, a total of 10 exotic fishes, including Pterygoplichthys anisitsi, which has never been reported from India found in the Ganges. Alterations of the hydrological pattern due to various types
of hydro projects was seems to be the largest threat to fishes of Ganges. Indiscriminate and illegal fishing, pollution, water
abstraction, siltation and invasion of exotic species are also threatening the fish diversity in the Ganges and as many as
29 species are listed under threatened category. The study advocates a need to identify critical fish habitats in the Ganga
basin to declare them as conservation reserves to mitigate the loss of fish diversity from this mighty large river. 相似文献
63.
Ali MA Ismail R Choon TS Yoon YK Wei AC Pandian S Kumar RS Osman H Manogaran E 《Bioorganic & medicinal chemistry letters》2010,20(23):7064-7066
Series of pyrolidine analogues were synthesized and examined as acetylcholinesterase (AChE) inhibitors. Among the compounds, compounds 4k and 6k were the most potent inhibitors of the series. Compound 4k, showed potent inhibitory activity against acetyl cholinesterase enzyme with IC(50) 0.10 μmol/L. Pyrolidine analogues might be potential acetyl cholinesterase agents for AD. 相似文献
64.
Selvaraj BarathManiKanth Kalimuthu Kalishwaralal Muthuirulappan Sriram SureshBabu Ram Kumar Pandian Hyung-seop Youn SooHyun Eom Sangiliyandi Gurunathan 《Journal of nanobiotechnology》2010,8(1):16
Background
Oxidative stress is imperative for its morbidity towards diabetic complications, where abnormal metabolic milieu as a result of hyperglycemia, leads to the onset of several complications. A biological antioxidant capable of inhibiting oxidative stress mediated diabetic progressions; during hyperglycemia is still the need of the era. The current study was performed to study the effect of biologically synthesized gold nanoparticles (AuNPs) to control the hyperglycemic conditions in streptozotocin induced diabetic mice. 相似文献65.
Laser backscattering from biological tissues depends on their composition and blood flow. The onset of the tissue abnormalities is associated with the change in composition at a specific location which may affect laser backscattering. The objective of the present work is to detect the compositional changes in tissue-equivalent phantom of fat, prepared by mixing paraffin wax with wax colors, and to characterize these in terms of their optical parameters. For this purpose these phantoms are scanned by a multi-probe non-contact automatic laser scanning system and images of the normalized backscattered intensity (NBI) are constructed. By scanning the background subtracted image of the phantom the location of the abnormality and its size from the full width at half maximum (FWHM) are determined. The data obtained by ultrasonic technique for localization of the abnormalities are in agreement with that as obtained by the present method. The optical parameters of the abnormality are obtained by matching the measured surface profiles of the abnormality with that of the profile obtained by Monte Carlo simulation. This analysis shows the possibility of detection of changes at the onset stage in tissues as required for planning of the photodynamic therapy. 相似文献
66.
Reini W Bretveld Chris MG Thomas Paul TJ Scheepers Gerhard A Zielhuis Nel Roeleveld 《Reproductive biology and endocrinology : RB&E》2006,4(1):30
Some pesticides may interfere with the female hormonal function, which may lead to negative effects on the reproductive system
through disruption of the hormonal balance necessary for proper functioning. Previous studies primarily focused on interference
with the estrogen and/or androgen receptor, but the hormonal function may be disrupted in many more ways through pesticide
exposure. The aim of this review is to give an overview of the various ways in which pesticides may disrupt the hormonal function
of the female reproductive system and in particular the ovarian cycle. Disruption can occur in all stages of hormonal regulation:
1. hormone synthesis; 2. hormone release and storage; 3. hormone transport and clearance; 4. hormone receptor recognition
and binding; 5. hormone postreceptor activation; 6. the thyroid function; and 7. the central nervous system. These mechanisms
are described for effects of pesticide exposure in vitro and on experimental animals in vivo. For the latter, potential effects of endocrine disrupting pesticides on the female reproductive system, i.e. modulation
of hormone concentrations, ovarian cycle irregularities, and impaired fertility, are also reviewed. In epidemiological studies,
exposure to pesticides has been associated with menstrual cycle disturbances, reduced fertility, prolonged time-to-pregnancy,
spontaneous abortion, stillbirths, and developmental defects, which may or may not be due to disruption of the female hormonal
function. Because pesticides comprise a large number of distinct substances with dissimilar structures and diverse toxicity,
it is most likely that several of the above-mentioned mechanisms are involved in the pathophysiological pathways explaining
the role of pesticide exposure in ovarian cycle disturbances, ultimately leading to fertility problems and other reproductive
effects. In future research, information on the ways in which pesticides may disrupt the hormonal function as described in
this review, can be used to generate specific hypotheses for studies on the effects of pesticides on the ovarian cycle, both
in toxicological and epidemiological settings. 相似文献
67.
Pandian RP Parinandi NL Ilangovan G Zweier JL Kuppusamy P 《Free radical biology & medicine》2003,35(9):1138-1148
The synthesis and characterization of a new lithium octa-n-butoxy-substituted naphthalocyanine radical probe (LiNc-BuO) and its use in the determination of concentration of oxygen (oximetry) by electron paramagnetic resonance (EPR) spectroscopy are reported. The probe is synthesized as a needle-shaped microcrystalline particulate. The particulate shows a single-line EPR spectrum that is highly exchange-narrowed with a line-width of 210 mG. The EPR line-width is sensitive to molecular oxygen showing a linear relationship between the line-width and concentration of oxygen (pO(2)) with a sensitivity of 8.5 mG/mmHg. We studied a variety of physicochemical and biological properties of LiNc-BuO particulates to evaluate the suitability of the probe for in vivo oximetry. The probe is unaffected by biological oxidoreductants, stable in tissues for several months, and can be successfully internalized in cells. We used this probe to monitor changes in concentration of oxygen in the normal muscle and RIF-1 tumor tissue of mice as a function of tumor growth. The data showed a rapid decrease in the tumor pO(2) with increase of tumor volume. Human arterial smooth muscle cells, upon internalization of the LiNc-BuO probe, showed a marked oxygen gradient across the cell membrane. In summary, the newly synthesized octa-n-butoxy derivative of lithium naphthalocyanine has unique properties that are useful for determining oxygen concentration in chemical and biological systems by EPR spectroscopy and also for magnetic tagging of cells. 相似文献
68.
Recombinant transformation vectors (ZPβypGH and ZpβrtGH) consisting of fish growth hormone cDNA, and a reporter geneβ-galactosidase driven by fish promoter (Zp) were constructed. Freshly fertilized eggs of zebrafish (Brachydanio rerio) were electroporated at optimum conditions (0.07 kV voltage; 25 μF capacitance; 8 ohm resistance and 2 pulses) in the presence
of one of these transformation vectors (100 μg circular DNNml). In either cases 72% of the electroporated eggs successfully
hatched, in comparison to the 85% hatchability of the control eggs. Genomic DNA extracted from fins of randomly chosenF
0 individuals was screened (by Southern blot hybridization); the transgenes were retained in the host genome of all the randomly
chosen adult transformants. Fin-positive presumptive founder parents were crossed with control counterparts and the DNA of
randomly chosenF
1 progenies was screened for germline transformation. Southern analysis of chosenF
1 progenies revealed the persistence of ZPβypGH or ZpβrtGH in 53% of theF
1 progenies. Southern analyses of chosenF
1 progenies and the frequency (53% ofF
1 ZpβrtGH and 53% ofF
1 ZP{β}ypGH) of transmission revealed the degree of mosaicism inF
0 transformants. Expression was confirmed from the 3–4 times elevated levels of activity of the reporter gene and 30–40% accelerated
growth of transgenicF
0 andF
1 progenies.
Construction of the transgenes was made at the Taiwan National Ocean University, Taiwan and all other work at the Madurai
Kamaraj University, Madurai. 相似文献
69.
R S English J S Lampel TJ Vanden Boom 《Journal of industrial microbiology & biotechnology》1998,21(4-5):219-224
The introduction of plasmid DNA into germinating spores of an industrially improved strain of Saccharopolyspora erythraea was accomplished by electroporation. Various parameters affecting the efficiency of electroporation were examined. The most
critical factor was the extent of spore germination. Electrocompetence was limited to a 4-h period following the initial emergence
of the germ tube. Electroporation efficiencies as high as 2 × 105 CFU μg−1 of plasmid DNA were obtained using electrocompetent germlings. The optimal field strength was 12–14 kV cm−1 with a pulse duration of 15–20 ms. Electrocompetent germlings were stored at −80°C without a significant decrease in transformation
efficiency. The utility of this protocol was demonstrated by isolating a propionyl-CoA carboxylase mutant through targeted
gene disruption and replacement.
Received 3 April 1998/ Accepted in revised form 28 September 1998 相似文献
70.
Production of Monoclonal Antibodies against the Major Capsid Protein of the Lactococcus Bacteriophage ul36 and Development of an Enzyme-Linked Immunosorbent Assay for Direct Phage Detection in Whey and Milk 总被引:4,自引:3,他引:1 下载免费PDF全文
Sylvain Moineau Denis Bernier Marie Jobin Jacques Hbert Todd R. Klaenhammer Sithian Pandian 《Applied microbiology》1993,59(7):2034-2040
The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs were selected and characterized. Western blots (immunoblots) showed that all MAbs recognized the 35 kDa but also a 45 kDa that is in lower concentration in the phage structure. Binding inhibition assays identified five families of MAbs that recognized nonoverlapping epitopes of the 35- and 45-kDa proteins. Immunoelectron microscopy showed that these two proteins are localized within the phage head, therefore indicating that the 35 kDa is a major capsid protein of ul36 and that the 45 kDa is a minor capsid protein. With two MAbs, a sandwich enzyme-linked immunosorbent assay (ELISA) was developed for direct detection of lactococcal phages in whey and milk samples. Whey and milk components, however, interfered with the conduct of the assay. Partial denaturation of milk samples by heat treatment in the presence of SDS and β-mercaptoethanol removed the masking effect and increased the sensitivity of the assay by 100-fold. With the method used here, 107 PFU/ml were detected by the ELISA within 2 h without any steps to enrich or isolate bacteriophages. 相似文献