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341.
The activity of both mitochondrial and cytosolic aconitases was significantly increased in the livers of male rats following treatment with the hypolipidemic drug clofibrate. Cycloheximide or puromycin administration to rats inhibited the inducing effect of clofibrate on the enzyme activity. Aconitase activity in small intestine homogenate was also increased by clofibrate. The drug did not affect the enzyme activity in rat kidney, heart and brain. 相似文献
342.
343.
L F Panchenko O S Brusov N A Beliaev 《Biulleten' eksperimental'no? biologii i meditsiny》1984,97(6):691-692
The influence of ethanol, its metabolites and some opiates on enkephalinase A activity was studied in rat experiments in vitro after acute and chronic administration of ethanol. It was demonstrated that addition of ethanol to the reaction mixture activated enkephalinase A of the midbrain and hypothalamus of intact rats, the maximal effect being attained at an ethanol concentration of 10(-3) M. Multiple washings with buffer of the ethanol-preincubated membranous fraction of these brain structures in the control rats did not lead to a significant reduction in the activating effect of ethanol on enkephalinase A. No activation was recorded upon the use of an enzymatic preparation of the brain from chronically alcoholized animals. Morphine, naltrexon, beta-carbolines, salsolinol (10(-4) M) and acetaldehyde (10(-8)-10(-2) M) did not activate the enzyme. It is suggested that enkephalinase A activation in rats given ethanol is determined by a direct action of ethanol on the enzyme. 相似文献
344.
The physical mechanisms of pressure influence on the protein dynamics and parameters of Mossbauer spectra were investigated. The pressure effects measured for human serum albumin using Rayleigh Scattering of Mossbauer Radiation techniques were described using the model of local diffusion. Parameters of this model were determined by analysing the experimental data. As a result an estimation of approximate values for activation volumes was performed. It was shown that the obtained values are well consistent with the previous experimental results. 相似文献
345.