Systematics of Mukdenia and Oresitrophe (Saxifragaceae): Insights from genome skimming data

Oresitrophe and Mukdenia (Saxifragaceae) are epilithic sister genera used in traditional Chinese medicine. The taxonomy of Mukdenia, especially of M. acanthifolia, has been controversial. To address this, we produced plastid and mitochondrial data using genome skimming for Mukdenia acanthifolia and Mukdenia rossii, including three individuals of each species. We assembled complete plastomes, mitochondrial CDS and nuclear ribosomal ETS/ITS sequences using these data. Comparative analysis shows that the plastomes of Mukdenia and Oresitrophe are relatively conservative in terms of genome size, structure, gene content, RNA editing sites and codon usage. Five plastid regions that represent hotspots of change (trnH-psbA, psbC-trnS, trnM-atpE, petA-psbJ and ccsA-ndhD) are identified within Mukdenia, and six regions (trnH-psbA, petN-psbM, trnM-atpE, rps16-trnQ, ycf1 and ndhF) contain a higher number of species-specific parsimony-informative sites that may serve as potential DNA barcodes for species identification. To infer phylogenetic relationships between Mukdenia and Oresitrophe, we combined our data with published data based on three different datasets. The monophyly of each species (Oresitrophe rupifraga, M. acanthifolia and M. rossii) and the inferred topology ((M. rossii, M. acanthifolia), O. rupifraga) are well supported in trees reconstructed using the complete plastome sequences, but M. acanthifolia and M. rossii did not form a separate clade in the trees based on ETS + ITS data, while the mitochondrial CDS trees are not well-resolved. We found low recovery of genes in the Angiosperms353 target enrichment panel from our unenriched genome skimming data. Hybridization or incomplete lineage sorting may be the cause of discordance between trees reconstructed from organellar and nuclear data. Considering its morphological distinctiveness and our molecular phylogenetic results, we strongly recommend that M. acanthifolia be treated as a distinct species.     

黄土高原部分地区180－73万年间孢粉植物群和环境演变初探

根据黄土高原３个剖面的孢粉资料及测年数据,恢复了本区１８０－７３万年期间的植被面貌。在此基础上,结合中国和全球其它地区的孢粉、构造、地层、水系演化和火山活动等特征。将此期间植被、气候和环境的演化划分为３个主旋回,６个主要演化阶段,１０个亚阶段和５个事件。初步认识到,该时间段的气候环境演化有较大波动,且这些变化不限于局部地区,而是区域性的,是多种因素（内外动力）作用的结果,且有规律可循。     

云南元谋小河地区古猿地点的小型猿类化石

本文记述的云南元谋小河地区古猿地点发现的一种小型猿类。它的牙齿形态比晚中新世的禄丰粗壮池猿进步。而牙齿的某些形态介于粗壮池猿和现生长臂猿之间,它的发现为探讨现生长臂猿的起源与进货提供了新的化石依据。依哺乳动物群的初步研究,其时代稍晚于禄丰古猿地点的时代。鉴于它的形态特征和地史分布,作者将它订为一新属新种：进步滇猿Ｄｉａｎｏ ｐｉｔｈｅｃｕｓ ｐｒｏｇｒｅｓｓｕｓ ｇｅｎ．ｅｔ ｓｐ．ｎｏｖ。     

湘鄂与闽粤猕猴颅骨的多变量分析

本文是中国猕猴湖南－湖北和广东－福建种群颅骨的多变量分析,结果表明,二者的面宽和颅肌的形态结构除有明显的性二型外,还存在较大的差异。湖南－湖北种群的颅骨结构相对大于福建广东种群。它们与云南南部和海南岛猕猴颅骨的判别分别结果表明,由于地理和生态隔离,四者雌雄两性颅骨的形态结构均有较大的差异,达到种群间的显著差异水平,分别应属不同的亚种。     

用正交实验法筛选营养缺陷型酿酒酵母原生质体的最佳实验条件

为了提高原生质体的再生率,用正交实验筛选了制备营养缺陷型酿酒酵母PW208菌株原生质体的条件。最佳条件为用柠檬酸缓冲液配制1％蜗牛酶,在30℃酶解60min后再经后处理可得99％的形成率和43％的再生率。     

中国山芹属一新种的研究

运用多学科综合分类手段,研究了山芹不同产地居群的变异,发现分布于我国东北部的各居群与华东居群不但存在地理分布上的间断,而且在外部形态、果实及叶柄解剖、核型组成和生物学特性等方面也存在显著差异,因而华东的山芹各居群应独立出来完成一新种──华东山芹OstericumhuadongensisZ.H.PanetX.H.L。     

粘质赛氏菌胞外蛋白酶的提取、纯化及部分性质研究

经过硫酸铵３０％～５０％分级沉淀、二步柱层析可获聚丙烯酰胺凝胶电泳均一的粘质赛氏菌胞外蛋白酶制品,收率可达５３％,并制备了酶的结晶,该酶以ＳｅｐｈａｄｅｘＧ１００柱层析及ＳＤＳ－ＰＡＧＥ测得分子量约为８１０００,该酶的最适ｐＨ为７．０,最适温度为４５℃,Ｚｎ２＋、Ｍｎ２＋、Ｆｅ２＋、Ｃｕ２＋、Ｃｏ２＋等重金属离子不同程度地抑制酶活性。     

Methodology in the study of seabass (Lates calcarifer Bloch): nutritional requirements in Singapore

This paper describes the effort of the Marine Aquaculture Section of the Primary Production Department in Singapore and the Institut Francais de Recherche pour l'Exploitation de la Mer in developing adequate facilities for fish nutritional requirement studies in Singapore, and in introducing appropriate nutritional methodology so that results are reliable and accurate. One of the first achievements was the demonstration of homogeneous fish (tropical seabass, Lates calcarifer Bloch) growth response in a newly established tank system before any nutritional experiments were initiated. The application of statistical procedures that take into consideration the power of a test and number of replications required has allowed for more accurate interpretation of results. This can be seen in the results of the first nutritional requirement experiment on the optimal dietary protein level of the seabass at constant energy.