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101.
ZhiXin Zhu AJ Harris Mir Muhammad Nizamani Andrew H. Thornhill Rosa A. Scherson HuaFeng Wang 《Ecology and evolution》2021,11(5):2100
To better identify biodiversity hotspots for conservation on Hainan Island, a tropical island in southern China, we assessed spatial variation in phylogenetic diversity and species richness using 18,976 georeferenced specimen records and a newly reconstructed molecular phylogeny of 957 native woody plants. Within this framework, we delineated bioregions based on vegetation composition and mapped areas of neoendemism and paleoendemism to identify areas of priority for conservation. Our results reveal that the southwest of Hainan is the most important hot spot for endemism and plant diversity followed by the southeast area. The distribution of endemic species showed a scattered, rather than clustered, pattern on the island. Based on phylogenetic range‐weighted turnover metrics, we delineated three major vegetational zones in Hainan. These largely correspond to natural secondary growth and managed forests (e.g., rubber and timber forests) in central Hainan, old‐growth forests and natural secondary growth forest at the margins of Hainan, and nature reserves on the island (e.g., Jianfeng and Diaoluo National Nature Reserves). Our study helps to elucidate potential botanical conservation priorities for Hainan within an evolutionary, phylogenetic framework. 相似文献
102.
Laurin AJ Mueller Karl G Kugler Michael Netzer Armin Graber Matthias Dehmer 《Biology direct》2011,6(1):1-15
Background
Chromosomal orthologs can reveal the shared ancestral gene set and their evolutionary trends. Additionally, physico-chemical properties of encoded proteins could provide information about functional adaptation and ecological niche requirements.Results
We analyzed 7080 genes (five groups of 1416 orthologs each) from Rhizobiales species (S. meliloti, R. etli, and M. loti, plant symbionts; A. tumefaciens, a plant pathogen; and B. melitensis, an animal pathogen). We evaluated their phylogenetic relationships and observed three main topologies. The first, with closer association of R. etli to A. tumefaciens; the second with R. etli closer to S. meliloti; and the third with A. tumefaciens and S. meliloti as the closest pair. This was not unusual, given the close relatedness of these three species. We calculated the synonymous (dS) and nonsynonymous (dN) substitution rates of these orthologs, and found that informational and metabolic functions showed relatively low dN rates; in contrast, genes from hypothetical functions and cellular processes showed high dN rates. An alternative measure of sequence variability, percentage of changes by species, was used to evaluate the most specific proportion of amino acid residues from alignments. When dN was compared with that measure a high correlation was obtained, revealing that much of evolutive information was extracted with the percentage of changes by species at the amino acid level. By analyzing the sequence variability of orthologs with a set of five properties (polarity, electrostatic charge, formation of secondary structures, molecular volume, and amino acid composition), we found that physico-chemical characteristics of proteins correlated with specific functional roles, and association of species did not follow their typical phylogeny, probably reflecting more adaptation to their life styles and niche preferences. In addition, orthologs with low dN rates had residues with more positive values of polarity, volume and electrostatic charge.Conclusions
These findings revealed that even when orthologs perform the same function in each genomic background, their sequences reveal important evolutionary tendencies and differences related to adaptation. This article was reviewed by: Dr. Purificación López-García, Prof. Jeffrey Townsend (nominated by Dr. J. Peter Gogarten), and Ms. Olga Kamneva. 相似文献103.
Visual acuity and contrast sensitivity progressively diminish with increasing viewing eccentricity. Here we evaluated how visual enumeration is affected by visual eccentricity, and whether subitizing capacity, the accurate enumeration of a small number (~3) of items, decreases with more eccentric viewing. Participants enumerated gratings whose (1) stimulus size was constant across eccentricity, and (2) whose stimulus size scaled by a cortical magnification factor across eccentricity. While we found that enumeration accuracy and precision decreased with increasing eccentricity, cortical magnification scaling of size neutralized the deleterious effects of increasing eccentricity. We found that size scaling did not affect subitizing capacities, which were nearly constant across all eccentricities. We also found that size scaling modulated the variation coefficients, a normalized metric of enumeration precision, defined as the standard deviation divided by the mean response. Our results show that the inaccuracy and imprecision associated with increasing viewing eccentricity is due to limitations in spatial resolution. Moreover, our results also support the notion that the precise number system is restricted to small numerosities (represented by the subitizing limit), while the approximate number system extends across both small and large numerosities (indexed by variation coefficients) at large eccentricities. 相似文献
104.
Adolfo Vásquez Trujillo Angélica E González Reina Agustín Góngora Orjuela Edgar Prieto Suárez Jairo Enrique Palomares Luz Stella Buitrago Alvarez 《Memórias do Instituto Oswaldo Cruz》2013,108(4):463-469
Lutzomyia antunesi has been commonly reported in outbreaks of
cutaneous leishmaniasis (CL) in the Orinoquia region of Colombia. The bionomics
of this species were studied in the municipality of Villavicencio (Meta,
Colombia). Sandflies were captured over the course of one week per month for one
year in intradomiciliary, peridomiciliary and extradomiciliary housing areas.
The captures were performed from 06:00 pm-06:00 am using CDC light traps and the
females were processed for polymerase chain reaction (PCR) to detect
Leishmania spp. A total of 22,097 specimens and 19 species
were captured of which Lu. antunesi (89%) and Lutzomyia
walkeri (5%) were the most abundant. Other species recognised as
anthropophilic (Lutzomyia panamensis, Lutzomyia
gomezi, Lutzomyia flaviscutellata and
Lutzomyia fairtigi) were present in very low abundance
(< 2%). Natural infection with Leishmania spp was detected using PCR in
Lu. antunesi, Lu. panamensis and
Lu. flavicutellata, showing infection rates of 1%, 4.8% and
7.5%, respectively. The present paper provides information on various ecological
aspects of Lu. antunesi. An analysis of seasonality shows that
this species increases in abundance in the hottest months (December, January and
February), directly correlating with the maximum temperature and inversely
correlating with precipitation. The natural infection rate is associated with
the peaks of highest abundance. 相似文献
105.
Egg strings and larvae of Hochstetter's frog (Leiopelma hochstetteri) were located at three widely separated North Island sites: in seeps at Brynderwyns in December 2004, in an open pool at Wharerino in March 2009, and in an underground pool near the Kaipawa Track, Coromandel, in late May 2009. Ten egg strings were also laid by captive frogs in water courses at Hamilton Zoo in April 2009. All egg strings held from 11 to 13 eggs. The egg strings laid in the Brynderwyns were regularly observed until metamorphosis was completed in March 2005. Twenty-four swimming larvae emerged from 25 capsules at c. 40 days after discovery, and at least 14 froglets were produced at c. 90 days. All of them developed in darkness, in a 120 ml pool <30 mm deep. The emerged froglets ranged from 9.8 to 10.8 mm snout-vent length. The detection of eggs, larvae and <11 mm froglets indicates that the egg laying period is at least from late September to May. 相似文献
106.
Laia Rodríguez-Revenga Elena Vallespín Irene Madrigal María Palomares Antonio Mur Sixto García-Miñaur Fernando Santos M. Ángeles Mori Pablo Lapunzina Montserrat Mila Julián Nevado 《Gene》2013
Developmental delay and intellectual disability, which occur in 1–3% of the population, account for a large number of the cases regularly seen in genetic units. Chromosomal microarray analysis has been shown to be a valuable clinical diagnostic assay and it should be the first-tier clinical diagnostic test for individuals with these conditions. However and due to several difficulties such as the platform resolution, the cost, and the inexperience with genomic data bases, the implementation of this test in many cytogenetic laboratories has been delayed. In an attempt to provide more insights of the benefits derived by using the chromosomal microarray analysis, this study presents the experience of two clinical centers using three different microarray platforms. The results obtained using a custom microarray (KaryoArray®) and two different commercial medium- and high-resolution whole-genome oligonucleotide microarrays have been compared. An overall diagnostic yield of around 15% has been obtained. However, the custom microarray platform has been shown to be more convenient for a clinical setting, since it allows the detection of more pathogenic copy number variants and less common variants. 相似文献
107.
108.
109.
Odón Vite-Vallejo Laura A. Palomares Edgar Dantán-González Hector G. Ayala-Castro Claudia Martínez-Anaya Brenda Valderrama Jorge Folch-Mallol 《Enzyme and microbial technology》2009,45(3):233-239
Protein glycosylation, a major post-translational modification, plays essential roles in eukaryotic cells. The glycosylation of fungal laccases has been proposed to be the bottleneck for the heterologous production of the enzyme, so it is important to determine its structure and function. We describe here the detailed N-glycosylation profile of Pycnoporus sanguineus laccase and its influence on some of its enzymatic properties. In this enzyme only high mannose structures were found, being those with 5- and 8-mannose units the most abundant. No other type of sugars was found in contrast to other fungal laccases. Enzymatic cleavage of the N-glycans present in the laccase provoked slight changes in the kinetic parameters, in the thermal stability and in the pH optimum of the enzyme. 相似文献
110.