It’s the first bites that count: Survival of first‐instar monarchs on milkweeds

Mortality of first instars is generally very high, but variable, and is caused by many factors, including physical and chemical plant characters, weather and natural enemies. Here, a summary of detailed field‐based studies of the early‐stage survival of a specialist lepidopteran herbivore is presented. First‐instar larvae of the monarch butterfly, Danaus plexippus, a milkweed specialist, generally grew faster and survived better on leaves when latex flow was reduced by partial severance of the leaf petiole. The outcome depended on milkweed species, and was related to the amount of latex produced, as well as other plant characters, such as leaf hairs, microclimate and concentration of secondary metabolites. Even for a so‐called ‘milkweed specialist’, larval performance and survival appears to be related to the concentration of cardenolides produced by the plants (a potential chemical defence against herbivory). This case study of monarchs and milkweeds highlights the need for field‐based experiments to assess the effect of plant characters on the usually poor survival of early instar phytophagous insects. Few similar studies concerning the performance and survival of first‐instar, eucalypt‐specific herbivores have been conducted, but this type of study is considered essential based on the findings obtained using D. plexippus.     

Influence of host log age and refuge from natural enemies on colonization and survival of Phoracantha semipunctata

The pine cone weevil, Pissodes validirostris Gyll. (Coleoptera: Curculionidae), attacks seed cones of most Eurasian pine species, except these of Swiss stone pine (Pinus cembra L.). Behavioural responses of adult weevils to cone volatile emissions of Swiss stone pine and to those of a common host, mountain pine (Pinus uncinata Ram.), were compared in an olfactometer. Weevils were significantly attracted by the volatile blend emitted by mountain pine, but Swiss stone pine volatiles elicited an inverse response, with most weevils moving in the opposite direction to the odour source. However, the majority of second instar weevil larvae that were extracted from mountain pine cones and transferred into Swiss stone pine cones were capable of developing to the adult stage. This suggests that Swiss stone pine cones do not contain strong feeding deterrents that could prevent larval development. The possible factors involved in the absence of colonization of Swiss stone pine cones by cone weevils are discussed.     

Regulated gene expression dictates enamel structure and tooth function.

Enamel is a complex bioceramic tissue. In its final form, enamel is a reflection of the unique molecular and cellular activities occurring during organogenesis. From the ectodermal origins of ameloblasts, their gene activity and protein expression profiles exist for the sole purpose of producing a mineralized shell, almost entirely devoid of protein, deposited over the 'bone-like' dentine. The interface between enamel and dentine is referred to as the dentine enamel junction and it is also unique in its biology. This review article is narrow in its scope. We restrict our review to selected advances in our understanding of the genetic, molecular and structural aspects of enamel biology. We present a model of enamel formation that relates gene expression to the assembly of an extracellular protein matrix that in turn controls the structural hierarchy and mechanical aspects of enamel and the tooth organ.     

Behavioral cues associated with oviposition by Trioza eugeniae

Trioza eugeniae oviposited significantly more eggs on Syzygium paniculatum leaves free of eggs compared to leaves with pre-existing eggs in a choice experiment, suggesting that females modify oviposition based on cues associated with the presence of eggs. In separate experiments, females avoided ovipositing on parts of leaves where eggs were present, but readily oviposited on areas of the same leaf without eggs. Epideictic pheromones are apparently not used by this species because females readily oviposited on areas of a leaf from which eggs had previously been removed. Females laid 57% of all their eggs along the margins of normal leaves, but also oviposited on artificial margins produced by cutting the leaves. The preference for natural margins over artificial margins was eliminated when the entire margin was coated with an antitranspirant compound to mask the damaged edge. These studies show that ovipositing psyllids respond both to the presence of eggs and to the suitability of the substrate.     

Evaluation of environmental and plant-associated cues for nymphal settling preference by Trioza eugeniae

Experiments were conducted to determine potential cues used by nymphs when they select the preferred abaxial side of a leaf. Nymphs most frequently settle and develop on the abaxial side of leaves. The degree of preference for the abaxial side was found to be reduced when a high density of nymphs were present, however. The orientation of leaves in a completely darkened chamber did not influence settling preference for the abaxial side, even when leaves were oriented abaxial side up. Coating both sides of a leaf with an antitranspirant compound did not prevent nymphs from preferring the abaxial side for settling. Similar results were obtained when leaves were dipped in pentane. These studies show that nymphs do not use gravity, color, light, or contact chemoreception when selecting suitable settling sites on a leaf.     

Influence of the larval environment on performance and adult body size of the wood-boring beetle Phoracantha semipunctata

Large body size confers a reproductive advantage to adults of the wood‐boring beetle Phoracantha semipunctata (F.) (Cerambycidae: Cerambycinae: Phoracanthini). Larvae of this species feed subcortically in stressed and dying eucalypt trees and logs. We evaluated the influence of the larval environment on larval performance and adult body size by manipulating the post‐felling age of host logs (from freshly cut to 2‐weeks‐old) and the density of colonizing neonates (low density with minimal competition for resources vs. high density with intense competition). Adult beetles emerged in greater numbers from logs that had been subjected to the aging treatment which reduced bark moisture content and favored colonization by neonates. Survival was greatest in larger logs having lower densities of neonates, but was greatly diminished in all treatments by mortality during pupation. Development time varied from 2 months to more than a year and was shortest in smaller logs having high densities of larvae. The size of adult beetles emerging from a log was not influenced by larval density, but was positively correlated with the age of logs when the neonates colonized, and log size. These findings suggest that the optimal developmental conditions for P. semipunctata larvae, in terms of larval performance and adult body size, are available in large, aged host logs having low densities of larvae. Manipulation of the larval environment in this study resulted in a considerable variation in adult body size, but large individuals were relatively more common in the wild population that was the source of neonates for the experiment. Potential body size may have been constrained by our use of only one host species and a narrow range of log dimensions.