Chromatography studies on bio-affinity of nine ligands of a1-adrenoceptor to a1D subtypes overexpressed in cell membrane

Copyright by Science in China Press 2004 The a1-AR is present in many tissues including heart, blood vessels, liver, brain, kidney, prostate and spleen. In these tissues, the a1-AR mediates a variety of physiological effects such as neurotransmission, vasoconstriction, cardiacinotropy, chronotropy and glycogenolysis[1]. In 1986, Morrow and Greese pro-posed that a1-AR should be subclassified into two subtypes. Later this hypothesis was proved by Q.D Han in 1987. He not only verified a1-A…     

二龄草鱼脾脏、肝脏组织高表达甘露糖结合凝集素mRNA

Innate immunity is expected to be very important in fish. Mannose-bingding lectin (MBL) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. In this experiment, total mRNA was isolated from spleen, liver, gills, thymus, head kidney and kidney of adult and immature grass carp Ctenopharygodon idllus. The cDNA of MBL was obtained by RT-PCR using total mRNA from the spleen of carp as template. Such cDNA was labled with ^32p and used as probe for Northern analysis, and autoradiographic signals were quantified by densitometry analysis. The results showed that MBL was high expressed in the spleen and liver and low in gills, thymus, head kidney and kidney of adult grass carp, and MBL was much lower expressed in spleen and liver of immature grass carp than those of adult grass carp. The results might partially explain why immature grass carp are vulnerable to grass carp hemorrhage virus (GCHV) whereas adult grass carp are not.This suggested that MBL mav be an imoortant anti-GCHV factor [Acta Zoologica Sinica 50 (1): 137 - 140. 2004].     

长春花激素完全适应型细胞的生长和阿玛碱合成特性

从长春花激素依赖型细胞系（C20D）筛选出一种激素完全适应型的细胞系（C20hi),考察了两种细胞生长、阿玛碱合成和引吲哚生物碱生物合成相关的酶的活性,结果表明：在生长培养基上二生长无显差异,而C20hi细胞平均阿玛碱含量是C20D的31.9倍,在生产培养基上C20hi细胞生长较C20D快,C20hi平均阿玛碱含量是C20D的18.4倍。通过比较生产和生长培养基中C20hi细胞的色氨酸脱羧酶、异胡豆苷合酶和long牛儿醇-10-脱氢酶活性,发明,通过5年的继代培养,激素完全适应型细胞系C20hi的阿玛碱含量是比较稳定的。