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71.
目的:探究类风湿性关节炎伴间质性肺病中肿瘤标志物对肺功能的影响。方法:选取2011年3月至2013年3月我院收治的类风湿性关节炎患者88例,根据其是否伴有ILD,分为RA组53例和RA-ILD组35例。检测两组肺功能指标用力肺活量(FVC)、1s用力呼气容积(FEV1)、最大通气量百分比(MVV)、一氧化碳弥散量(DLCO),肿瘤标志物癌胚抗原CEA、癌抗原125(CA125)、癌抗原199(CA199)及抗环胍氨酸肽抗体(抗CCP抗体)的值,并利用统计学方法分析肿瘤标记物与肺功能指标、抗CCP抗体的相关性。结果:RA.ILD组FVC、FEV1、MVV、DLCO均比RA组低,CEA、CA125、CA199均比RA组高,结果比较差异显著具有统计学意义(P〈0.05)。但两组抗CCP抗体含量相比却无明显差异,不具有统计学意义(P〉0.05)。相关性分析显示,CEA与FVC、FEV1、MVV、DLCO呈负相关(P〈0.05),而CA125、CA199却与肺功能指标无相关性,CEA、CA125、CA199与抗CCP抗体均无相关性(P〉0.05)。结论:RA-ILD的肺功能指标均有明显下降,而肿瘤标志物表达水平却明显增高,CEA对肺功能损害影响较大,却与关节损害的关系不大,这为临床对类风湿性关节炎伴间质性肺病早诊断、早治疗提供了依据。 相似文献
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Shu Xu Jianying Luo Xiayan Pan Xiaoyu Liang Jian Wu Wenjun Zheng Changjun Chen Yiping Hou Hongyu Ma Mingguo Zhou 《Biochimica et Biophysica Acta - Proteins and Proteomics》2013,1834(8):1660-1670
The plant-pathogenic bacterium Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight, which is one of the most serious diseases of rice. Xoo has been studied for over one century, and much has been learned about it, but proteomic investigation has been neglected. In this study, proteome reference maps of Xoo were constructed by two-dimensional gel electrophoresis, and 628 spots in the gels representing 469 different protein species were identified with MALDI-TOF/TOF MS. The identified spots were assigned to 15 functional categories according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the annotations from the National Center for Biotechnology Information (NCBI) database. The data set has been deposited in the World-2DPAGE database (Database ID: 0044). In addition, comparative proteomic analysis revealed that proteins related to the TonB-dependent transportation system and energy metabolism are involved in the phenazine-1-carboxylic acid resistance in Xoo. In conclusion, we have established a proteome database for Xoo and have used this database in a comparative proteomic analysis that identified proteins potentially contributing to phenazine-1-carboxylic acid resistance in Xoo. 相似文献
74.
Lingling Wang Yufu Tang Shuo Liu Shitao Mao Yuan Ling Dan Liu Xiaoyu He Xiaoge Wang 《PloS one》2013,8(6)
Chronic obstructive pulmonary disease (COPD) has seriously impacted the health of individuals and populations. In this study, proton nuclear magnetic resonance (1H NMR)-based metabonomics combined with multivariate pattern recognition analysis was applied to investigate the metabolic signatures of patients with COPD. Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Samples were analyzed by high resolution 1H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis. Consistent metabolic differences have been found in serum as well as in urine samples from COPD patients and healthy controls. Compared to healthy controls, COPD patients displayed decreased lipoprotein and amino acids, including branched-chain amino acids (BCAAs), and increased glycerolphosphocholine in serum. Moreover, metabolic differences in urine were more significant than in serum. Decreased urinary 1-methylnicotinamide, creatinine and lactate have been discovered in COPD patients in comparison with healthy controls. Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects. Our results illustrate the potential application of NMR-based metabonomics in early diagnosis and understanding the mechanisms of COPD. 相似文献
75.
Genome-wide analysis of gene-gene interactions has been recognized as a powerful avenue to identify the missing genetic components that can not be detected by using current single-point association analysis. Recently, several model-free methods (e.g. the commonly used information based metrics and several logistic regression-based metrics) were developed for detecting non-linear dependence between genetic loci, but they are potentially at the risk of inflated false positive error, in particular when the main effects at one or both loci are salient. In this study, we proposed two conditional entropy-based metrics to challenge this limitation. Extensive simulations demonstrated that the two proposed metrics, provided the disease is rare, could maintain consistently correct false positive rate. In the scenarios for a common disease, our proposed metrics achieved better or comparable control of false positive error, compared to four previously proposed model-free metrics. In terms of power, our methods outperformed several competing metrics in a range of common disease models. Furthermore, in real data analyses, both metrics succeeded in detecting interactions and were competitive with the originally reported results or the logistic regression approaches. In conclusion, the proposed conditional entropy-based metrics are promising as alternatives to current model-based approaches for detecting genuine epistatic effects. 相似文献
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Xiaofei Lv Yuan Zhang Shaoqi Rao Dongfang Su Dan Feng Min Wang Xinrui Li Dan Li Honghui Guo Xiaoyu Zuo Min Xia Haimei Ouyang Wenhua Ling Jian Qiu 《Gene》2013
Studies focusing on the association of gene methylthioadenosine phosphorylase (MTAP) with the risk of coronary artery disease (CAD) and myocardial infarction (MI) are limited. 相似文献
79.
Bei Wen Longfei Li Yu Liu Hongna Zhang Xiaoyu Hu Xiao-quan Shan Shuzhen Zhang 《Plant and Soil》2013,362(1-2):345-354
Aims
There is a need to predict trace metal concentration in plant organs at given development stages. The aim of this work was to describe the Cd hyperaccumulation kinetics in the different plant organs, throughout the complete cultivation cycle, independently of a possible soil effect.Methods
Plants of Noccaea caerulescens were exposed in aeroponics to three constantly low Cd concentrations and harvested at 6 to 11 dates, until siliquae formation.Results
Dry matter allocation between roots and shoots was constant over time and exposure concentrations, as well as Cd allocation. However 86 % of the Cd taken up was allocated to the shoots. Senescent rosette leaves showed similar Cd concentrations to the living ones, suggesting no redistribution from old to young organs. The Cd root influx was proportional to the exposure concentration and constant over time, indicating that plant development had no effect on this. The bio-concentration factor (BCF), i.e. [Cd]plant/[Cd2+]solution for the whole plant, roots or shoots was independent of the exposure concentration and of the plant stage.Conclusions
Cadmium uptake in a given plant part could therefore be predicted at any plant stage by multiplying the plant part dry matter by the corresponding BCF and the Cd2+ concentration in the exposure solution. 相似文献80.
A metagenomic library containing 8,000 clones was constructed by using genomic DNA obtained from Chaerhan Salt Lake in northwest China. Three Na+/H+ antiporters, C4-NhaG, C47-NhaG and C49-NhaG that grouped to the NhaG family, were screened and cloned from this metagenome by complementing Escherichia coli strain KNabc (ΔnhaA ΔnhaB ΔchaA) in medium containing 0.2 M NaCl. The three putative Na+/H+ antiporters were membrane proteins with 10, 11 and 11 transmembrane segments, respectively. They enabled E. coli KNabc to grow in medium containing 0.2–0.6 M Na+ or 7–14 mM Li+. Everted membrane vesicles prepared from E. coli KNabc cells carrying C49-NhaG exhibited Na+/H+ and Li+/H+ antiport activities. 相似文献