不同类型杂交水稻组合及其亲本苗期同工酶位点分析

对6个杂交水稻组合及其亲本苗期14种酶的同工酶进行检测,并对其中较为清晰稳定的酶带进行了位点分析,以探讨杂种优势遗传基础。共获得了28个位点的相关资料,包括每个位点的性质、表型多态性和每个位点所编码的酶活性及酶带平均迁移率。所有供试材料在其中25个位点上均未表现出多态性,只有部分组合与其亲本在Est-4、Amy-1、Aat-2等3个位点上存在多态性,其中有81．8％的差异组合其酶带表现出共显性特征。对共显性与杂种优势的关系进行了探讨。     

DNA足纹步移作图法

一种以PCR介导的、可对任意长度靶DNA片段上的核蛋白结合位点进行DNA足纹作图分析的新方法.原理是：采用被随机降解靶DNA分子作为模板,用标记的跨越整个模板的足够多条特异性引物进行单链扩增.首先,利用某种化学试剂或酶如DNaseⅠ对已与蛋白质结合或未结合的双链靶DNA进行随机降解,在一定条件下使每一个DNA分子恰好只有一个位点被切割.然后,这些被随机降解DNA分子即可作为模板,用同位素标记的跨越整个模板的足够多条特异性引物（正向或反向）进行单链扩增.最后,扩增的单链产物通过变性聚丙烯酰胺凝胶电泳和放射自显影形成DNA片段梯队,而被蛋白质保护的位点则在DNA片段梯队中形成位置缺口,从而确定DNA与蛋白质相结合的精确位点.该方法被应用对人干细胞因子基因5′旁侧－1190～－273区域的DNA足纹部分作图.     

基因快速检测仪的自动进样控制系统的研制

基因检测是遗传工程的重要技术之一,基因检测技术的自动化对工程的研究具有重要意义,而自动进样控制系统是基因快速检测仪的重要组成部分。该系统的硬件部分主要由液位检测电路及其接口电路组成;软件部分主要由VisualC 6．0编程对硬件实现其自动控制功能。该系统主要包括：控制清洗通道流程、排除废流流程和进样流程等功能,工作模式可根据人机对话方式设定,并能将扩增反应后的试样自动送到基因检测池中进行检测。系统具有快速、操作方便、智能化程度高、准确性高等特点。     

Phenotypic Profiling of Scedosporium aurantiacum,an Opportunistic Pathogen Colonizing Human Lungs

Genotyping studies of Australian Scedosporium isolates have revealed the strong prevalence of a recently described species: Scedosporium aurantiacum. In addition to occurring in the environment, this fungus is also known to colonise the respiratory tracts of cystic fibrosis (CF) patients. A high throughput Phenotype Microarray (PM) analysis using 94 assorted substrates (sugars, amino acids, hexose-acids and carboxylic acids) was carried out for four isolates exhibiting different levels of virulence, determined using a Galleria mellonella infection model. A significant difference was observed in the substrate utilisation patterns of strains displaying differential virulence. For example, certain sugars such as sucrose (saccharose) were utilised only by low virulence strains whereas some sugar derivatives such as D-turanose promoted respiration only in the more virulent strains. Strains with a higher level of virulence also displayed flexibility and metabolic adaptability at two different temperature conditions tested (28 and 37°C). Phenotype microarray data were integrated with the whole-genome sequence data of S. aurantiacum to reconstruct a pathway map for the metabolism of selected substrates to further elucidate differences between the strains.     

U-Shaped Photonic Crystal Fiber Based Surface Plasmon Resonance Sensors

A surface plasmon resonance sensor based on a U-shaped photonic crystal fiber with a rectangular lattice has been designed through finite element method. The U-shaped fiber exhibits not only stronger mechanical strength but also better sensor performance than our previous scheme. The upper detection limit extends to higher analyze refractive index, 1.384, for phase interrogation. We introduce a ratio to evaluate the impact of higher order plasmonic mode. For wavelength modulation scheme, the parameter to describe the performance of a sensor is chosen to be the figure of merit, which can be up to 533.8[RIU^?1] around complete coupling condition.     

miR‐124a inhibits the proliferation and inflammation in rheumatoid arthritis fibroblast‐like synoviocytes via targeting PIK3/NF‐κB pathway

Abnormal hyperplasia of fibroblast‐like synoviocytes (FLS) leads to the progression of rheumatoid arthritis (RA). This study aimed to investigate the role of miR‐124a in the pathogenesis of RA. The viability and cell cycle of FLS in rheumatoid arthritis (RAFLS) were evaluated by Cell Counting Kit 8 and flow cytometry assay. The expression of PIK3CA, Akt, and NF‐κB in RAFLS was examined by real‐time PCR and Western blot analysis. The production of tumour necrosis factor (TNF)‐α and interleukin (IL)‐6 was detected by ELISA. The joint swelling and inflammation in collagen‐induced arthritis (CIA) mice were examined by histological and immunohistochemical analysis. We found that miR‐124a suppressed the viability and proliferation of RAFLS and increased the percentage of cells in the G1 phase. miR‐124a suppressed PIK3CA 3'UTR luciferase reporter activity and decreased the expression of PIK3CA at mRNA and protein levels. Furthermore, miR‐124a inhibited the expression of the key components of the PIK3/Akt/NF‐κB signal pathway and inhibited the expression of pro‐inflammatory factors TNF‐α and IL‐6. Local overexpression of miR‐124a in the joints of CIA mice inhibited inflammation and promoted apoptosis in FLS by decreasing PIK3CA expression. In conclusion, miR‐124a inhibits the proliferation and inflammation in RAFLS via targeting PIK3/NF‐κB pathway. miR‐124a is a promising therapeutic target for RA.     

Reproductive allocation of Solidago canadensis L. plays a key role in its invasiveness across a gradient of invasion degrees

Reproductive allocation (RA) plays a vital role in the development of ecological strategies during the life cycle of plant species. Invasive alien plants (IAP) may exist at various invasion degrees across a gradient of the colonization process with several grades of relative abundances in the occupied environments. The progressive variation in the invasion degree of IAP has the potential to modify their RA strategy. This study purposes of estimating the RA strategy of the IAP Solidago canadensis L. and the correlations among RA of S. canadensis, the invasion intensity of S. canadensis, the invasiveness of S. canadensis, and the community invasibility across a gradient of invasion degrees by using the field sampling experiment. The height and relative abundance of S. canadensis did not remarkably affect its RA. The RA of S. canadensis was positively related to its reproductive biomass and total biomass. The key reason may be that plant individuals with higher total biomass can allocate more resources into sexual reproduction. The RA of S. canadensis was positively related to its invasiveness. Thus, the RA of S. canadensis may be crucial to its invasiveness.