Antisense EGFR sequence reverses the growth properties of human liver carcinoma cell line BEL-7404 in vitro

A recombinant plasmid containing a full length human epidermal growth factor receptor (EGFR) cDNA sequence in antisense orientation was transferred into cells of a human liver carcinoma cell line BEL-7404. Compared with the control cell clone JX-0 transferred with the vector plasmid and the parent BEL-7404 cells, the antisense EGFR transferred cell clone JX-1 showed a decreased EGFR gene expression and reduced significantly the growth potential either in anchorage-dependent or anchorage-independent growth. Furthermore, JX-1 cells appeared to be distinctly dependent on serum concentration for monolayer growth. The results suggested that antisense EGFR could partly block the EGFR gene expression and reverse the malignant growth properties of human liver carcinoma cells in vitro.     

人红细胞膜上阴离子交换蛋白和葡萄糖运输蛋白的相互影响

用细胞松弛素Ｂ抑制红细胞膜上葡萄糖运输蛋白（ＧｌｕＴ－１）对葡萄糖的运输,观察到对阴离子运输有促进作用。当ＧｌｕＴ－１结合其底物分子－葡萄糖后同样加快了阴离子运输速率．另一方面,实验办给出了葡萄糖跨膜运输特性和Ｃｌ－离子浓度的关系,表明随着Ｃｌ－离子浓度的加大能使葡萄糖运输过程中Ｋｍ减小、Ｖ（ｍａｘ）增大。这些结果表明了在完整红细胞膜上阴离子交换蛋白Ｂａｎｄ３和ＧｌｕＴ－１之间存在着双向联系,即一种膜蛋白的构象改变能影响另一种膜蛋白的功能。     

中国异皮属线虫一新种（线虫纲：垫刃目：异皮科）

本文描述采自河北省滦县寄生于白茅Ｉｍｐｅｒａｔｅ ｃｙｌｉｎｄｒｉｃａ ｖａｒ．Ｍａｊｏｒ的异皮属线虫一新种,中华异皮线虫Ｈｅｔｅｒｏｄｅｒａ ｓｉｎｅｎｓｉｓ ｓｐ．ｎｏｖ．;新种孢囊为柠檬形,阴门膜孔为两侧单膜孔型,下桥发达有泡状突;二龄幼虫口针基部球前部略凹陷,口孔侧唇外缘融合王头晕第１环纹内,即无明显侧唇,其体侧区有３条侧线与体环纹呈不完全网格状交叉;雄虫侧区有３条侧线与体环纹呈完全网格状     

环境中富啡酸对小麦吸收利用亚硒酸钠及其毒性的影响

利用小麦水培安验,研究了从土壤中提取的富啡酸（Ｆｕｌｖｉｃａｃｉｄ,ＦＡ）对亚硒酸钠生物有效性及毒性的影响。结果表明,富啡酸对小麦吸收利用亚硒酸钠有明显的影晌,并且对高浓度亚硒酸钠的毒性有一定的抑制作用,富啡酸可提高小麦对硒胁迫的生态适应性。     

Sequence variation and genetic diversity in the giant panda

About 336–444 bp mitochondrial D-loop region and tRNA gene were sequenced for 40 individuals of the giant panda which were collected from Mabian, Meigu, Yuexi, Baoxing, Pingwu, Qingchuan, Nanping and Baishuijiang, respectively. 9 haplotypes were found in 21 founders. The results showed that the giant panda has low genetic variations, and that there is no notable genetic isolation among geographical populations. The ancestor of the living giant panda population perhaps appeared in the late Pleistocene, and unfortunately, might have suffered bottleneck attacks. Afterwards, its genetic diversity seemed to recover to some extent. Project supported by the “8.5” Key Project of Chinese Academy of Sciences, the Chairman Foundation of Chinese Academy of Sciences, K. C. Wang Education Foundation, the Applied Basic Research Foundation of Yunnan, the National Natural Science Foundation of China, the Special Foundation for Returned Chinese Scientists, and Zoological Society of San Diego.     

中国管蛛属一新种：蜘蛛目：圆颚蛛科

本文记述采自江西省修水县的管蛛属蜘蛛一新种：中华管蛛Ｔｒａｃｈｅｌａｓｓｉｎｅｎｓｉｓｓｐ．ｎｏｖ．。该种与日本管蛛Ｔ．ｊａｐｏｎｉｃａＢｏｅｓ．ｅｔＳｔｒ．,１９０６在体型,体色及腹部背面的斑纹上非常相似,但该种的身体明显大于后者,两者外生殖器存在明显的差异。     

从大鼠肝染色质富集具有转录活性的DNA

采用DNase消化法从大鼠肝染色质分离得到富有转录活性的DNA(sDNA)。sDNA琼脂糖凝胶电泳,在0.5—6Kb范围内背景有一片荧光,小于1Kb范围内,出现明显区带。sDNA为探针与大鼠正常肝核RNA杂交百分数(29.5%),为以总核DNA为探针杂交百分数(8.2%)的3.6倍,并高于sDNA与大鼠肝癌核RNA杂交百分数(16.4%).     

人工补充寄主卵对松林内卵蜂种群消长的影响

在不同生境的松林中,人工补充寄主卵都能提高寄生效果。但林地生境不同,寄生率有明显差异。在松阔混交林中补充寄主,其寄生率比对照提高5.5—16.2倍。植被稀疏的纯松林效果较差,补充的寄生率比对照提高3.0倍。 卵蜂种群消长随季节温度而变化,全年以5月中旬至6月下旬和9月中旬至10月中旬为两个寄生高峰。卵蜂种群与松毛虫种群的消长存在较明显的相依关系,卵蜂种群随着松毛虫种群的消长而消长。施药对卵蜂种群有较大影响,施药区比对照区的寄生率约降低一倍。在混交林中填充寄主卵,能促进卵蜂种群世代延续。在逐步改善林地生境的基础上,利用人工补充寄主,可以代替人工繁蜂放蜂。     

散白蚁属两新种(等翅目:鼻白蚁科)

中国散白蚁属种类十分丰富,已发表的种类近20种,分属两个亚属,近年来湖南省林业部门结合普查工作又采得不少白蚁标本,经整理鉴定,其中鼻白蚁科的散白蚁属有两个新种。均属于额白蚁亚属。现描述如下。模式标本保存于中国科学院动物研究所。 平江散白蚁Reticulitermes(Frontotermes)pingjiangensis Tsai et Peng新种 兵蚁(图1—5)体中型,头部橙黄色,上颚棕褐色,颚基赤黄色,触角堤脊褐黄色,触角及胸部背板乳黄色,腹部色泽浅淡,体被小毛。     

龙牙百合3个地方品种的形态特征及核型分析

采用经典测量和染色体常规压片法,对龙牙百合（Lilium brownii var.viridulum Baker）3个地方品种的形态特征及核型进行研究。植株形态分析结果显示：‘江西’龙牙的株高、开花口径、种球重量和周长、中外层鳞片重量和长度以及鳞片扦插产生小鳞茎数等指标均显著大于‘大叶’龙牙和‘平头’龙牙;‘大叶’龙牙的叶片最长,均值为14.54 cm。花粉、叶表皮气孔及鳞片淀粉粒的微形态特征分析结果显示：‘江西’龙牙的花粉粒径最大,均值达111.76 μm;‘平头’龙牙的叶表皮气孔最长,气孔密度也最大（约47.6个/mm²）;‘大叶’龙牙的淀粉粒径最大,均值为47.61 μm;‘江西’龙牙的淀粉粒大小分布更集中,差异性小。染色体核型分析结果显示：龙牙百合3个品种的染色体数目均为2n=2x=24,为二倍体,其中‘江西’龙牙核型公式为2n=2x=24=2m（2SAT）+6sm（2SAT）+12st（4SAT）+4t;‘平头’龙牙核型公式为2n=2x=24=4m+8sm+10st（4SAT）+2t;‘大叶’龙牙核型公式为2n=2x=24=2m（2SAT）+6sm+14st（4SAT）+2t,三者核型均为3B型。