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51.
We isolated six polymorphic microsatellite loci in the migratory freshwater fish Prochilodus costatus, which is an endemic species and important fisheries resource from São Francisco river basin, Brazil. We have evaluated the applicability of these loci to study genetic variation in wild population of this fish. Thus, based on the genotypes of 32–48 individuals, we detected two to 21 alleles per locus, observed and expected heterozygosities ranging from 0.19 to 0.89 and from 0.17 to 0.92, respectively. These polymorphic markers should provide efficient tools to study population genetic structure of this fish.  相似文献   
52.
This paper analyses relationships between relative growth rate ( rgr ), seed mass, biomass allocation, photosynthetic rate and other plant traits as well as habitat factors (rainfall and altitude) in 20 wild species of Aegilops L. and one closely related species of Amblyopyrum (Jaub. & Spach) Eig., which differ in ploidy level (diploid, tetraploid and hexaploid). The plants were grown hydroponically for 20 d in a growth chamber. The relationships between parameters were calculated either using the phylogenetic information (phylogenetically independent contrasts, PIC) or without using the phylogenetic information (trait values of taxa, TIP). The results using the two approaches were very similar, but there were a few exceptions in which the results were different (e.g. rgr vs. seed mass). Specific leaf area ( sla ) was positively correlated with leaf area ratio ( lar ) and negatively correlated with net assimilation rate ( nar ), which together resulted in the absence of a correlation between sla and rgr . Leaf photosynthetic rates (expressed on a mass or area basis) showed no correlation with rgr . rgr was positively correlated with the stem mass ratio and negatively with root mass ratio. Species with a lower d. wt percentage have a higher rgr . Aegilops species from locations with higher annual rainfall invested less biomass in roots and more in shoots (leaves and stems) and had a higher rgr . Diploid species had a lower seed mass and initial mass than the hybrids (tetraploid and hexaploid species), but there was no correlation of rgr with ploidy level. Polyploid species, which have higher seed mass, occur at a higher altitude than diploid species. Our results show that variation in rgr in Aegilops and Amblyopyrum spp. is associated mainly with variation in biomass allocation (proportion of biomass in stems and roots) and d. wt percentage, and not with variation in sla , leaf photosynthetic rates or seed mass.  相似文献   
53.
1. The degree to which communities are variable may be affected by the ecological conditions to which they are exposed and can affect their propensity to form alternative states. We examined the influence of two common ecological factors, predation and seasonal successional stage, on the variability in community composition of herbivorous pond plankton. In a highly replicated, two factor, mesocosm experiment we determined whether beta diversity was affected by seasonal successional stage of the community (two levels), by fish predation (presence/absence) or by their interaction. 2. Several significant changes were found in the composition of the rotifer, cladoceran and copepod assemblages. Most cladoceran abundances showed sharp declines in the presence of fish, while some rotifers, as well as their assemblage species richness, responded favourably to fish. The copepod assemblage was composed of omnivorous and carnivorous species, which added invertebrate predation to the experiment and which intensified as the season progressed. Copepods showed responses to fish predation that depended on seasonal successional stage of the initial community, because of changes in their stage structure and edibility as they grew from nauplii to adults. 3. Community variability was consistently high at the end of each month‐long experimental period for both cladoceran and rotifer assemblages, except under two conditions. In the early season treatments, the rotifer assemblages were more consistent (lower beta diversity) in the presence of fish. This was attributed to high population growth rates for rotifers under these ecological conditions because of reduced copepod predation on them through a trophic cascade from fish. Low community variability was also observed in the late season for rotifers when fish were excluded and, as a result, they were exposed to high invertebrate predation from cyclopoid copepods. 4. Results from the early season support theoretical predictions that when community size increases, variability in composition should decline because of an increase in competitive processes over stochastic ones. Late season results suggest that a second mechanism, specialist predation, can also reduce prey community variability. Our study demonstrates that plankton communities may be more predictable under certain trophic web configurations and challenges ecologists to find ways to incorporate such inherent variability into experiments and community theory.  相似文献   
54.
Abstract: We used remotely triggered cameras to collect data on Puma (Puma concolor) abundance and occupancy in an area of tropical forest in Brazil where the species' status is poorly known. To evaluate factors influencing puma occupancy we used data from 5 sampling campaigns in 3 consecutive years (2005 to 2007) and 2 seasons (wet and dry), at a state park and a private forest reserve. We estimated puma numbers and density for the 2007 sampling data by developing a standardized individual identification method. We based individual identification on 1) time-stable parameters (SP; physical features that do not change over time), and 2) time-variable parameters (VP; marks that could change over time such as scars and botfly marks). Following individual identification we established a capture-recapture history and analyzed it using closed population capture-mark-recapture models. Puma capture probability was influenced by camera placement (roads vs. trails), sampling year, and prey richness. Puma occupancy was positively associated with species richness and there was a correlation between relative puma and jaguar (Panthera onca) abundance. Identifications enabled us to generate 8 VP histories for each photographed flank, corresponding to 8 individuals. We estimated the sampled population at 9 pumas (SE = 1.03, 95% CI = 8–10 individuals) translating to a density of 3.40 pumas/100 km2. Information collected using camera-traps can effectively be used to assess puma population size in tropical forests. As habitat progressively disappears and South American felines become more vulnerable, our results support the critical importance of private forest reserves for conservation.  相似文献   
55.
56.
Ecological features and conservation requirements of populations at the latitudinal limits of a species’ geographical range frequently differ from those in other parts of the range. Identifying such differences is key to implementing effective conservation strategies for threatened range‐edge populations especially, in the context of rapid global warming, at the lower‐latitude range edge. We studied habitat selection and diet of the endangered Cantabrian Capercaillie Tetrao urogallus cantabricus in a recently discovered population at the southernmost edge of the sub‐species’ range. This is the only Western Capercaillie population in the Mediterranean biogeographical region. We combined non‐systematic surveys based on questionnaires, reports and field sampling with data from radiotracking to assess habitat selection. Diet was surveyed by micro‐histological methods from droppings collected in the new population, which inhabits Pyrenean Oak Quercus pyrenaica forests and Scots Pine Pinus sylvestris plantations, and in two Cantabrian populations inhabiting Eurosiberian forests. Capercaillie preferred large (> 500 ha) and medium‐sized (100–500 ha) Pyrenean Oak forest fragments and large Scots Pine plantations. Forest fragments smaller than 100 ha and non‐forested habitats were always avoided. Diet differed markedly between Mediterranean and Eurosiberian populations. Bilberry Vaccinium myrtillus is common in the diet of most Capercaillie populations but was scarce in the study area and so was rare in the diet of the new population. Instead, Rockrose Halimium lasianthum was described for the first time as a major food resource for the Capercaillie and was consumed in autumn and winter. Pine needles were also heavily consumed in winter. We document for the first time the strong preference of Capercaillie for Pyrenean Oak forests and a moderately high consumption of the leaves, buds and acorns of this tree species throughout the year. Habitat selection and diet of this Mediterranean population differ from those of the core Cantabrian and other populations. Our results suggest a wider environmental tolerance (phenotypic plasticity) in the species than previously recognized. We advocate specific protection for this unique range‐edge Capercaillie population and its Pyrenean Oak forest habitat.  相似文献   
57.
The genetic consequences of population fragmentation and isolation are major issues in conservation biology. In this study we analyse the genetic variability and structure of the Iberian populations of Mioscirtus wagneri, a specialized grasshopper exclusively inhabiting highly fragmented hypersaline low grounds. For this purpose we have used seven species‐specific microsatellite markers to type 478 individuals from 24 localities and obtain accurate estimates of their genetic variability. Genetic diversity was relatively low and we detected genetic signatures suggesting that certain populations of M. wagneri have probably passed through severe demographic bottlenecks. We have found that the populations of this grasshopper show a strong genetic structure even at small geographical scales, indicating that they mostly behave as isolated populations with low levels of gene flow among them. Thus, several populations can be regarded as independent and genetically differentiated units which require adequate conservation strategies to avoid eventual extinctions that in highly isolated localities are not likely to be compensated for with the arrival of immigrants from neighbouring populations. Overall, our results show that these populations probably represent the ‘fragments’ of a formerly more widespread population and highlight the importance of protecting Iberian hypersaline environments due to the high number of rare and endangered species they sustain.  相似文献   
58.
1. This study analysed changes occurring in Mediterranean stream fish assemblages over a sequence of dry years followed by a generally wet period (1991–98). Variations in assemblage attributes were quantified at the basin and stream reach scales, and related to variables reflecting the occurrence of unusually dry or wet conditions. 2. Assemblage variability increased along with the resolution of analysis, with little changes in species richness, composition and rank abundances, but significant variation in individual species abundances. Fluctuations in relative abundances were significantly affected by variables reflecting the severity of summer droughts and the occurrence of rainy springs. These patterns were evident at the basin scale, while variability at individual stream reaches tended to be higher and less related to rainfall patterns. 3. At least three response guilds to rainfall variation could be identified: two of the four abundant and widespread species (chub and loach) declined following dry years, whereas the two other core species (nase and eel) declined after rainy spring; one scarce native species (stickleback) increased in dry years. 4. Except at the two most upstream reaches, the assemblages tended to recover quickly to previous configuration after the changes occurring during the sequence of dry years. 5. Temporal variability of local assemblages was concordant among reaches but did not follow any consistent spatial pattern. Instead, spatial patterns in assemblage attributes changed over time in response to environmental variability, with a tendency for a disruption of upstream–downstream gradients following dry years. 6. Results supported the view that present‐day droughts cause relatively small and transient changes to Mediterranean stream fish assemblages. However, longer and more severe droughts expected under altered future climates, may result in declines or local extinctions of the most sensitive species and their potential replacement by more resistant species. Changing drought regimes thus need to be duly considered in the development of conservation strategies for Mediterranean stream fish.  相似文献   
59.
Molecular markers are frequently used to study genetic variation among individuals within or between populations. Differences in marker banding patterns can be used to verify if individuals do, or do not, represent distinct groups or populations. Only in 2005, more than 500 studies used molecular markers to group individuals in clusters. Such studies make use of an arbitrary number of molecular markers from each of an arbitrary number of individuals presumed to represent distinct genotypes. However, the greater the genetic variation, the more likely a larger number of individuals and markers will be needed to capture a population's genetic signature. The numbers of both, markers and individuals included thus affect the way in which individuals are organized through cluster analyses, thereby affecting the conclusions drawn. Here we present a method that provides statistical criteria to verify that individual and marker sample sizes are sufficient to accurately depict genetic differentiation among different populations. Our method uses a resampling technique to assess the reproducibility of obtaining a particular grouping pattern for specific data sets. It thus, allows to estimate the robustness of the results obtained without including additional individuals, or markers.  相似文献   
60.
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