Climatic fluctuations and orogenesis as motors for speciation in East Africa: case study on Parepistaurus Karsch, 1896 (Orthoptera)

Mechanisms of speciation of flightless grasshoppers in mountainous and coastal East Africa are inferred considering (i) phylogenies estimated with a combination of molecular markers (16S rRNA locus, COI and H3), (ii) ecological data and (iii) the geographic distribution of Parepistaurus species. The study suggests that coastal taxa of Parepistaurus belong to ancestral lineages from which evolved the high diversity of species found in the Eastern Arc Mountains of Tanzania and Kenya, which are geologically ancient mountain formations. Network analyses and a molecular clock approach, calibrated with the geological age of the volcanoes, suggested that speciation was boosted by climatic fluctuations affecting large areas of East Africa. With the aridification beginning 2.8 Ma, forest taxa were isolated due to forest fragmentation and populations were separated by extended grasslands, which are avoided by Parepistaurus species. However, a humid period between 2.7 and 2.5 Ma triggered a spread of coastal taxa along the Eastern Arc Mountains. Forests expanded again and riparian vegetation along rivers draining into the Indian Ocean probably served as corridors for the dispersal of coastal taxa to the hinterland. The inland volcanoes such as Mount Kilimanjaro are therefore good time markers because their geological age is known, limiting the available time for speciation processes of mountainous Parepistaurus in the area to a maximum of about 1–2 Ma. A third humid but cold period between 1.1 and 0.9 Ma probably further boosted the spread of several flightless and montane‐adapted Orthoptera taxa.     

Mutational analysis of the <Emphasis Type="Italic">GLA</Emphasis> gene in Mexican families with Fabry disease

Fabry disease (FD) is a lysosomal storage disorder, which develops due to a deficiency in the hydrolytic enzyme, α-galactosidase A (α-Gal A). Alpha-Gal A hydrolyzes glycosphingolipid globotriaosylceramide (Gb3), and an α-Gal A deficiency leads to Gb3 accumulation in tissues and cells in the body. This pathology is likely to involve multiple systems, but it is generally considered to affect primarily vascular endothelium. In this study, we investigated mutations in the GLA gene, which encodes α-Gal A, in Mexican families with FD. We included seven probands with FD that carried known mutations. We analysed pedigrees of the probands, and performed molecular screening in 65 relatives with the potential of carrying a GLA mutation. Five mutations (P40S, IVS4 ⁺⁴, G328V, R363H, R404del) were detected in seven unrelated Mexican families with the classic FD phenotype. Of the 65 relatives examined, 42 (64.6%) had a GLA gene mutation. In summary, among seven Mexican probands with FD, 65 relatives were at risk of carrying a known GLA mutation, and molecular screening identified 42 individuals with the mutation. Thus, our findings showed that it is important to perform molecular analysis in families with FD to detect mutations and to provide accurate diagnoses for individuals that could be affected.     

TRACING BACK THE ORIGIN OF THE INDO-PACIFIC MOLLUSC FAUNA: BASAL TRIDACNINAE FROM THE OLIGOCENE AND MIOCENE OF THE SULTANATE OF OMAN

Abstract:  Two new tridacnine species are described from the Chattian and Aquitanian of the Arabian Peninsula. For these, the new names Omanidacna eos gen. et sp. nov. and Tridacna evae sp. nov. are erected. Omanidacna is interpreted as an Oligocene ancestor of Hippopus , being the oldest record of this tridacnine lineage. The Aquitanian Tridacna evae is the first occurrence of the genus Tridacna . These Arabian taxa imply that the modern tridacnine lineages are rooted in the Palaeogene and early Neogene of the East African-Arabian Province, although their Eocene ancestors, such as Byssocardium , are Western Tethyan taxa. During the Neogene they successfully settled the Indo-Polynesian Province and became typical elements of the entire Indo-West Pacific Region. The tridacnines are thus an example of a successive transformation and gradual eastward dispersal of an originally Tethyan element contributing to late Neogene diversity in the Indo-West Pacific.     

Cytogenetics of Bolinus brandaris and phylogenetic inferences within the Muricidae (Mollusca: Gastropoda)

The purple dye murex, Bolinus brandaris (Linnaeus, 1758), is a muricid gastropod common throughout the Mediterranean and along the Moroccan and Portuguese Atlantic coasts. In the present study, we confirmed the diploid chromosome number of 2 n  = 70 for this species, and established for the first time the karyotype, which comprised 12 metacentric, 15 submetacentric and eight subtelocentric chromosome pairs. To facilitate cytotaxonomic comparisons, we carried out a comparative karyological analysis through multidimensional scaling between B. brandaris and three other 2 n  = 70 muricid species ( Hexaplex trunculus , Ocenebra erinaceus , and Stramonita haemastoma ) for which chromosomal measurements have been previously published. The interpretation of the ideograms and the statistical analysis highlighted the closest similarity of B. brandaris and H. trunculus compared to S. haemastoma and O. erinaceus . Indeed, B. brandaris and H. trunculus showed the smallest dissimilarities both for relative length and arm ratio, with O. erinaceus presenting intermediate values, whereas the highest dissimilarities were found between H. trunculus and S. haemastoma for both data. The karyotypes of B. brandaris and H. trunculus (subfamily Muricinae) presented the highest proportions of metacentric chromosomes compared to the other two muricids analysed, suggesting that those karyotypes could be considered primitive within the 2 n  = 70 Muricidae studied so far.  © 2009 The Linnean Society of London, Biological Journal of the Linnean Society , 2009, 96 , 185–193.     

The genus Troglophilus Krauss, 1879 (Orthoptera: Rhaphidophoridae) in the west Balkans

We present a revision of the genus Troglophilus in the western part of the Balkan Peninsula based on morphological and genetic analysis, and provide distribution data for the species. The results of these analyses reduced the number of the known taxa within the genus Troglophilus in this area to five valid species: Troglophilus cavicola ( Kollar, 1833 ), Troglophilus neglectus Krauss, 1879 , Troglophilus ovuliformis Karny, 1907 , Troglophilus brevicauda Chopard, 1934, and Troglophilus lazaropolensis Karaman, 1958. A new species Troglophilus zorae sp. nov. is described and Troglophilus neglectus serbicus Maran, 1958, Troglophilus neglectus vlasinensis Maran, 1958, Troglophilus bukoviki Karaman, 1968 and Troglophilus pretneri Us, 1970 are synonymized. The distribution pattern of the western Balkan species supports a general east?west migration route. We also determined the centres of origin and spreading directions of particular species according to the distribution of the taxa and the structure of the haplotypes. © 2011 The Linnean Society of London, Zoological Journal of the Linnean Society, 2011, 163 , 1035–1063.     

Nitrate Uptake and Reduction of Aseptically Cultivated Spruce Seedlings, Picea abies (L.) Karst

Spruce (Picea abies (L.) Karst.) seedlings were asepticallycultivated and the effects of different N-nutrition on net uptakeand reduction of nitrate were investigated. The characteristicsof nitrate uptake were calculated, K_s as 0?2 mol m^–3 andV_max as 18 µmol g^–1 d^–1. Low pH, and Al³⁺ in the medium caused adecrease in nitrate uptake rate. An in vivo assay was set upwhich allowed the measurement of NRA in both roots and needlesof spruce seedlings. The in vivo nitrate reductase activitywas repressed by ammonium and stimulated by nitrate. Nitratereduction was similar to nitrate uptake, negatively affectedby low pH and ammonium. Therefore, a limited N-supply to spruceseemed to occur when pH was low in the rhizosphere combinedwith the presence of Al³⁺ and . Key words: Spruce, nitrate uptake, nitrate reduction     

Analysis of the heterochromatin of <Emphasis Type="Italic">Cebus</Emphasis> (Primates,Platyrrhini) by micro-FISH and banding pattern comparisons

The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences.     

AN INQUIRY INTO THE PRINCIPLES OF NEEDS‐BASED ALLOCATION OF HEALTH CARE

The concept of need is often proposed as providing an additional or alternative criterion to cost‐effectiveness in making allocation decisions in health care. If it is to be of practical value it must be sufficiently precisely characterized to be useful to decision makers. This will require both an account of how degree of need for an intervention is to be determined and a prioritization rule that clarifies how degree of need and the cost of the intervention interact in determining the relative priority of the intervention. Three common features of health care interventions must be accommodated in a comprehensive theory of need: the probabilistic nature of prognosis (with and without the intervention); the time course of effects; and the fact that the most effective treatments often combine more than one intervention. These common features are problematic for the concept of need. We outline various approaches to prioritization on the basis of need and argue that some approaches are more promising than others.     

Herpetomonas roitmani (Fiorini et al., 1989) N. Comb.: A Trypanosomatid with a Bacterium-like Endosymbiont in the Cytoplasm

The trypanosomatid previously described as Crithidia roitmani is characterized here at the ultrastructural and biochemical levels. The data indicates that the parasite belongs to the Herpetomonas genus, and we therefore suggest the flagellate to be denominated as Herpetomonas roitmani n. comb. Cladistic analysis of isoenzyme data generated by eight different enzymes showed that the parasite presented a distinct banding pattern and could be grouped with some Herpetomonas spp., but not with Crithidia spp., used as reference strains. Accordingly, when the parasites were grown for longer periods in Roitman's defined medium, expontaneous differentiation from promastigotes to opisthomastigotes (typical of the Herpetomonas genus) occurred. Transmission electron microscopy revealed the presence of bacterium-like endosymbionts in the cytoplasm of all evolutive forms of the parasite. All morphological alterations characteristic of endosymbiont-bearing trypanosomatids could be observed.