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111.
Summary Protein X, molecular weight 40,000, has been separated from the other proteins of E. coliby a two-dimensional gel electrophoretic technique which separates proteins according to isoelectric point (pI) in the firstdimension and according to molecular weight in the second. When protein X is induced in wild-type cells by mitomycin C treatmentit has a pI6.0. However, when protein X is induced in a tif-1 mutant, either by temperatureshift-up to 42° or by mitomycin C treatment at 30°, it has a pI6.2. The low level of protein X which is present inuninduced tif mutants at 30° also has a pI6.2. These results suggest thattif-1 is a mis-sense mutation in the gene coding for protein X. Since transduction andcomplementation studies indicate that tif-1 is a mutation of therecA
+
gene (Castellazzi, Morand, George and Buttin, 1977) it follows that protein X is the recA
+ gene product.A model has been formulated to account for the relationship between protein X synthesis and the recA
+
and lexA
+
genes. In this model, a repressor coded by lexA
+
binds to the operator of the recA
+
gene from whence it can normally only be removed by the combined action of an inducer and protein X, the recA
+ product. Thus, protein X controls its own synthesis. The tif-1 mutation leads to a temperature sensitive form of protein X which, at 42°, can spontaneously remove the repressor without the intervention of the inducer. 相似文献
112.
113.
114.
Water use and sodium chloride uptake by apple trees 总被引:2,自引:0,他引:2
D. W. West 《Plant and Soil》1978,50(1-3):37-49
Summary Apple trees grown with their root systems split into halves were used to study the effects of non-uniform salinity stress
within a root system upon salt and water uptake.
Water uptake declined rapidly when sodium chloride solution (90 meq l−1) was added to any root zone but uptake increased correspondingly in the non-saline root zone of each tree. This changed pattern
of water uptake with partial salinization did not change the total water use by the trees compared with their water use when
neither root zone was salt stressed. After a‘steady-state’ condition of water uptake had been reached 80 to 85% of the water
was taken up in the non-saline root zone.
Irrigation at three soil matric potential intervals of −6.6, −33 and −66 kPa allowed to develop in the non-saline root zone
of each tree did not affect water use responses.
Leaf concentrations of Ca, Mg and K were unaffected by treatments. Chloride and Na concentrations increased in leaves with
exposure to salinity stress in half root zones and with increasing soil matric potential stress.
Some evidence was obtained using tritium enriched water that water was transferred from a non-saline root zone into a saline
root zone but the volume involved was unmeasurable. 相似文献
115.
116.
Purified liver microsomal NADPH-cytochrome P-450 reductase is able to catalyze the activation of [14C]ronidazole to metabolite(s) which bind covalently to protein. Like the reaction catalyzed by microsomes, protein alkylation catalyzed by the reductase is (1) sensitive to oxygen, (2) requires reducing equivalents, (3) is inhibited by sulfhydryl-containing compounds and (4) is stimulated several fold by either flavin mononucleotide (FMN) or methytlviologen. A cytochrome P-450 dependent pathway of ronidazole activation can be demonstrated as judged by the inhibition of the reaction by carbon monoxide, metyrapone and 2,4-dichloro-6-phenylphenoxyethylamine but the involvement of specific microsomal cytochrome P-450 isozymes has not been definitively established. Milk xanthine oxidase is also capable of catalyzing ronidazole activation. Polyacrylamide sodium dodecyl sulfate (SDS)-gel electrophoresis reveals that the reactive intermediate(s) of ronidazole does not alkylate proteins selectively. 相似文献
117.
Ronidazole (1-methyl-5-nitroimidazole-2-methanol carbamate) is reductively metabolized by liver microsomal and purified NADPH-cytochrome P-450 reductase preparations to reactive metabolites that covalently bind to tissue proteins. Kinetic experiments and studies employing immobilized cysteine or blocked cysteine thiols have shown that the principal targets of protein alkylation ara cysteine thiols. Furthermore, ronidazole specifically radiolabelled with 14C in the 4,5-ring, N-methyl or 2-methylene positions give rise to equivalent apparent covalent binding suggesting that the imidazole nucleus is retained in the bound residue. In contrast, the carbonyl-14C-labeled ronidazole gives approx. 6--15-fold less apparent covalent binding indicating that the carbamoyl group is lost during the reaction leading to the covalently bound metabolite. The conversion of ronidazole to reactive metabolite(s) is quantitative and reflects the amazing efficiency by which this compound is activated by microsomal enzymes. However, only about 5% of this metabolite can be accounted for as protein-bound products under the conditions employed in these studies. Consequently, approx. 95% of the reactive ronidazole metabolite(s) can react with other constituents in the reaction media such as other thiols or water. Based on these results, a mechanism is proposed for the metabolic activation of ronidazole. 相似文献
118.
A numerical taxonomic study of species of Vibrio isolated from the aquatic environment and birds in Kent, England 总被引:4,自引:3,他引:1
A numerical taxonomic study has been carried out to confirm the identity of strains of the family Vibrionaceae isolated during an ecological study. A total of 237 strains were studied including 148 from the aquatic environment, 6 from estuarine birds, 1 from sheep faeces, and 61 control cultures. Duplicates of 21 of the strains were randomly selected and included to estimate test and operator error. Taxonomic resemblance was estimated on the basis of 148 characters using Euclidean distance. The taxonomic position of some strains was reevaluated using the pattern difference coefficient. Strains were clustered by three methods, all of which gave similar results. The estimated average probability of test error was 1.5%. Strains previously identified as Vibrio anguillarum fell into four distinct phenons corresponding to V. anguillarum biovar I, ' V. anguillarum biovar II', V. diazotrophicus , and strains pathogenic to oyster larvae. The latter group characteristically degraded xanthine and probably represents a new species. The phenon corresponding to V. cholerae included the type strain, strains of human origin, and strains isolated in the United Kingdom from birds and the aquatic environment. Some strains of V. cholerae were luminous. Other phenons were identified as V. metschnikovii, V. fluvialis , and Aeromonas spp. 相似文献
119.
Barometric pressures at extreme altitudes on Mt. Everest: physiological significance 总被引:2,自引:0,他引:2
West J. B.; Lahiri S.; Maret K. H.; Peters R. M. Jr; Pizzo C. J. 《Journal of applied physiology》1983,54(5):1188-1194
120.
Characterization of Mutant Alleles of Myospheroid, the Gene Encoding the β Subunit of the Drosophila PS Integrins
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T. A. Bunch S. Salatino M. C. Engelsgjerd L. Mukai R. F. West D. L. Brower 《Genetics》1992,132(2):519-528
This paper presents the characterization of nine alleles of myospheroid, which encodes the beta PS subunit of the Drosophila PS integrins. On Southern blots, the mysXB87, mysXN101 and mysXR04 genes yield restriction digest patterns similar to that seen for wild-type chromosomes, however the mys1 and mysXG43 genes contain detectable deletions. mys1, mysXB87 and mysXG43 make little or no stable protein product, and genetically behave as strong lethal alleles. For the mysXN101 mutation, protein product is seen on immunoblots and a reduced amount of beta PS protein is seen at muscle attachment sites of embryos; this mutant protein retains some wild-type function, as revealed by complementation tests with weak alleles. Protein is also seen on immunoblots from mysXR04 embryos, and this allele behaves as an antimorph, being more deleterious in some crosses than the complete deficiency for the locus. mysts2 and mysnj42 are typically lethal in various combinations with other alleles at high temperatures only, but even at high physiological temperatures, neither appears to eliminate gene function completely. The complementation behaviors of mysts1 and mysts3 are quite unusual and suggest that these mutations involve regulatory phenomena. For mysts3, the data are most easily explained by postulating transvection effects at the locus. The results for mysts1 are less straightforward, but point to the possibility of a chromosome pairing-dependent negative interaction. 相似文献