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101.
Many autotrophs vary their allocation to nutrient uptake in response to environmental cues, yet the dynamics of this plasticity
are largely unknown. Plasticity dynamics affect the extent of single versus multiple nutrient limitation and thus have implications
for plant ecology and biogeochemical cycling. Here we use a model of two essential nutrients cycling through autotrophs and
the environment to determine conditions under which different plastic or fixed nutrient uptake strategies are adaptive. Our
model includes environment-independent costs of being plastic, environment-dependent costs proportional to the rate of plastic
change, and costs of being mismatched to the environment, the last of which is experienced by both fixed and plastic types.
In equilibrium environments, environment-independent costs of being plastic select for tortoise strategies—fixed or less plastic
types—provided that they are sufficiently close to co-limitation. At intermediate levels of environmental fluctuation forced
by periodic nutrient inputs, more hare-like plastic strategies prevail because they remain near co-limitation. However, the
fastest is not necessarily the best. The most adaptive strategy is an intermediate level of plasticity that keeps pace with
environmental fluctuations, but is not faster. At high levels of environmental fluctuation, the environment-dependent cost
of changing rapidly to keep pace with the environment becomes prohibitive and tortoise strategies again dominate. The existence
and location of these thresholds depend on plasticity costs and rate, which are largely unknown empirically. These results
suggest that the expectations for single nutrient limitation versus co-limitation and therefore biogeochemical cycling and
autotroph community dynamics depend on environmental heterogeneity and plasticity costs. 相似文献
102.
Walter H. Adey H. Dail Laughinghouse IV John B. Miller Lee‐Ann C. Hayek Jesse G. Thompson Steven Bertman Kristin Hampel Shanmugam Puvanendran 《Journal of phycology》2013,49(3):489-501
Two Algal Turf Scrubber (ATS) units were deployed on the Great Wicomico River (GWR) for 22 months to examine the role of substrate in increasing algal productivity and nutrient removal. The yearly mean productivity of flat ATS screens was 15.4 g · m?2 · d?1. This was elevated to 39.6 g · m?2 · d?1 with a three‐dimensional (3‐D) screen, and to 47.7 g · m?2 · d?1 by avoiding high summer harvest temperatures. These methods enhanced nutrient removal (N, P) in algal biomass by 3.5 times. Eighty‐six algal taxa (Ochrophyta [diatoms], Chlorophyta [green algae], and Cyan‐obacteria [blue–green algae]) self‐seeded from the GWR and demonstrated yearly cycling. Silica (SiO2) content of the algal biomass ranged from 30% to 50% of total biomass; phosphorus, nitrogen, and carbon content of the total algal biomass ranged from 0.15% to 0.21%, 2.13% to 2.89%, and 20.0% to 25.7%, respectively. Carbohydrate content (at 10%–25% of AFDM) was dominated by glucose. Lipids (fatty acid methyl ester; FAMEs) ranged widely from 0.5% to 9% AFDM, with Omega‐3 fatty acids a consistent component. Mathematical modeling of algal produ‐ctivity as a function of temperature, light, and substrate showed a proportionality of 4:3:3, resp‐ectively. Under landscape ATS operation, substrate manipulation provides a considerable opportunity to increase ATS productivity, water quality amelioration, and biomass coproduction for fertilizers, fermentation energy, and omega‐3 products. Based on the 3‐D prod‐uctivity and algal chemical composition demonstrated, ATS systems used for nonpoint source water treat‐ment can produce ethanol (butanol) at 5.8× per unit area of corn, and biodiesel at 12.0× per unit area of soy beans (agricultural production US). 相似文献
103.
104.
Tobacco callus grown under shoot-forming conditions or in the presence of gibberellic acid, which inhibits shoot formation, was incubated in [14 C]-sucrose at three different periods in culture and then replanted. Evolution of 14 CO2 occurred during the 10 day post-incubation period. Most of the radioactivity was incorporated into the ethanol-soluble fraction, which lost most of its label after 24 h. Starch was the major ethanol-insoluble component and post-incubation synthesis occurred in this fraction for 24 h or longer. Greater net synthesis of starch occurred in shoot-forming tissue and the loss of label from starch began later than in tissue cultured in the presence of gibbe-rellic acid. Newly synthesized starch was not immediately utilised in the organogenic process, but its utilization could be correlated with the shoot-forming process. 相似文献
105.
The observed range for temporal mean-variance scaling exponents can be explained by reproductive correlation 总被引:1,自引:0,他引:1
The mean-variance scaling relationship known as Taylor's power law has been well documented empirically over the past four decades but a general theoretical explanation for the phenomenon does not exist. Here we provide an explanation that relates empirical patterns of temporal mean-variance scaling to individual level reproductive behavior. Initially, we review the scaling behavior of population growth models to establish theoretical limits for the scaling exponent b that is in agreement with the empirically observed range (1≤b≤2). We go on to show that the degree of reproductive covariance among individuals determines the scaling exponent b. Independent reproduction results in an exponent of one, while completely correlated reproduction results in the upper limit of two. Intermediate exponents, which are common empirically, can be generated through the decay of reproductive covariance with increasing population size. Finally, we describe how the link between reproductive correlation and the scaling exponent provides a way to infer properties of individual-level reproductive behavior, such as the relative influence of demographic stochasticity, from a macroecological pattern. 相似文献
106.
Thomas K. Barthel Douglas R. Mathern G. Kerr Whitfield Carol A. Haussler H. Andrew Hopper IV Jui-Cheng Hsieh Stephanie A. Slater Grace Hsieh Magdalena Kaczmarska Peter W. Jurutka Olga I. Kolek Fayez K. Ghishan Mark R. Haussler 《The Journal of steroid biochemistry and molecular biology》2007,103(3-5):381
107.
Ward G. Walkup IV Lorraine Washburn Michael J. Sweredoski Holly J. Carlisle Robert L. Graham Sonja Hess Mary B. Kennedy 《The Journal of biological chemistry》2015,290(8):4908-4927
synGAP is a neuron-specific Ras and Rap GTPase-activating protein (GAP) found in high concentrations in the postsynaptic density (PSD) fraction from the mammalian forebrain. We have previously shown that, in situ in the PSD fraction or in recombinant form in Sf9 cell membranes, synGAP is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII), another prominent component of the PSD. Here, we show that recombinant synGAP (r-synGAP), lacking 102 residues at the N terminus, can be purified in soluble form and is phosphorylated by cyclin-dependent kinase 5 (CDK5) as well as by CaMKII. Phosphorylation of r-synGAP by CaMKII increases its HRas GAP activity by 25% and its Rap1 GAP activity by 76%. Conversely, phosphorylation by CDK5 increases r-synGAP''s HRas GAP activity by 98% and its Rap1 GAP activity by 20%. Thus, phosphorylation by both kinases increases synGAP activity; CaMKII shifts the relative GAP activity toward inactivation of Rap1, and CDK5 shifts the relative activity toward inactivation of HRas. GAP activity toward Rap2 is not altered by phosphorylation by either kinase. CDK5 phosphorylates synGAP primarily at two sites, Ser-773 and Ser-802. Phosphorylation at Ser-773 inhibits r-synGAP activity, and phosphorylation at Ser-802 increases it. However, the net effect of concurrent phosphorylation of both sites, Ser-773 and Ser-802, is an increase in GAP activity. synGAP is phosphorylated at Ser-773 and Ser-802 in the PSD fraction, and its phosphorylation by CDK5 and CaMKII is differentially regulated by activation of NMDA-type glutamate receptors in cultured neurons. 相似文献
108.
The Malagasy endemic ant Malagidris sofina (Bolton and Fisher 2014) nests on cliff faces in natural rock alcoves or clay banks. Colonies have single ergatoid queens and reproduce by fission. Each nest has a funnel-shaped entrance that projects horizontally from the cliff face. We examine three hypotheses for the function of the funnels—water exclusion, gas exchange and defense. Entrance funnels are relatively impermeable and divert water from nests, but simple tubes would achieve the same result. Consistent with the gas exchange hypothesis, projected funnel entrances likely increase gas exchange rates over sixfold compared to simple tubes and may increase air flow within the nest. Gas exchange may explain the recurrent evolution of funnel entrances in several ant lineages, especially among cliff dwelling species. We outline M. sofina defense responses to conspecifics and co-occurring ant species, and find no support for a defense role of entrance funnels. Workers display little aggression but respond to several species with an original form of nest defense––cliff jumping—in which workers drop off the cliff face while clinging to invaders and then return to their nest. M. sofina is a restricted range species under threat of extinction by habitat destruction. Its novel lifestyle underscores the urgency of exploration and conservation in a tropical biodiversity hotspot. 相似文献
109.
Shipeng Wei Bryan C. Roessler Sylvain Chauvet Jingyu Guo John L. Hartman IV Kevin L. Kirk 《The Journal of biological chemistry》2014,289(29):19942-19957
ATP-binding cassette (ABC) transporters are an ancient family of transmembrane proteins that utilize ATPase activity to move substrates across cell membranes. The ABCC subfamily of the ABC transporters includes active drug exporters (the multidrug resistance proteins (MRPs)) and a unique ATP-gated ion channel (cystic fibrosis transmembrane conductance regulator (CFTR)). The CFTR channel shares gating principles with conventional ligand-gated ion channels, but the allosteric network that couples ATP binding at its nucleotide binding domains (NBDs) with conformational changes in its transmembrane helices (TMs) is poorly defined. It is also unclear whether the mechanisms that govern CFTR gating are conserved with the thermodynamically distinct MRPs. Here we report a new class of gain of function (GOF) mutation of a conserved proline at the base of the pore-lining TM6. Multiple substitutions of this proline promoted ATP-free CFTR activity and activation by the weak agonist, 5′-adenylyl-β,γ-imidodiphosphate (AMP-PNP). TM6 proline mutations exhibited additive GOF effects when combined with a previously reported GOF mutation located in an outer collar of TMs that surrounds the pore-lining TMs. Each TM substitution allosterically rescued the ATP sensitivity of CFTR gating when introduced into an NBD mutant with defective ATP binding. Both classes of GOF mutations also rescued defective drug export by a yeast MRP (Yor1p) with ATP binding defects in its NBDs. We conclude that the conserved TM6 proline helps set the energy barrier to both CFTR channel opening and MRP-mediated drug efflux and that CFTR channels and MRP pumps utilize similar allosteric mechanisms for coupling conformational changes in their translocation pathways to ATP binding at their NBDs. 相似文献
110.
Susann Weissmueller Eusebio Manchado Michael Saborowski John P. Morris IV Elvin Wagenblast Carrie A. Davis Sung-Hwan Moon Neil T. Pfister Darjus F. Tschaharganeh Thomas Kitzing Daniela Aust Elke K. Markert Jianmin Wu Sean M. Grimmond Christian Pilarsky Carol Prives Andrew V. Biankin Scott W. Lowe 《Cell》2014