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81.
Ayse Karatug Engin Kaptan Sehnaz Bolkent Ozgur Mutlu Refiye Yanardag 《Journal of trace elements in medicine and biology》2013,27(1):52-57
Diabetes mellitus is a chronic disease characterized by anomalies forming in carbohydrate, lipid, protein metabolisms and the incidence of this disease varies widely throughout the world. Zinc is an important element which is essential for life and is present in nature. In this study, the animals were divided into four groups. These groups were named as untreated; zinc sulfate; streptozotocin (STZ); STZ and zinc sulfate. STZ (65 mg/kg) was dissolved in a freshly prepared 0.01 M pH 4.5 citrate buffer and given with intraperitoneal injection in a single dose. Zinc sulfate (100 mg/kg) was dissolved in distilled water and given to the animals by gavage at a daily dose for 60 days. The rats were sacrificed under ether anesthesia. This study was aimed to investigate histological and biochemical changes of zinc supplementation on the kidney tissue in STZ-induced diabetic rats. In the current study, histological and histochemical observations showed that the occurred degenerative changes decreased after giving zinc in the kidney tissue of diabetic group. Kidney glutathione (GSH) levels decreased and lipid peroxidation (LPO), nonenzymatic glycosylation (NEG), urea and creatinine levels increased in diabetic rats. GSH levels increased, while LPO, NEG, urea and creatinine levels decreased in the kidney with administration of zinc to diabetic rats. As a result, we observed curative effects of zinc given to diabetic rats. We can say that zinc may be an important antioxidant for the treatment of secondary complications of diabetes in kidney tissue. 相似文献
82.
Ceyhun SB Aksakal E Ekinci D Erdoğan O Beydemir Ş 《Biological trace element research》2011,144(1-3):781-789
The present research aims to evaluate the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on metallothioneins and cytocrome P450. Mature rainbow trouts were exposed to 10?mg/L CoCl(2).6H(2)O and 1?mg/L ZnSO(4).7H(2)O. After 6, 12, 24, and 48?h of treatment, expressions of muscle MT-A, MT-B, and CYP P4501A1 mRNAs were measured by means of quantitative real-time polymerase chain reaction. During the exposure experiments, no mortalities occurred. We observed that expression levels of all genes increased with exposure time. Since the organism has not learned how to completely dispose of heavy metals and tends to bioaccumulate them, our results indicate that cobalt and zinc exposure may result in accumulation of the non-eliminated metals which may lead to fish death. 相似文献
83.
Ekinci D Ceyhun SB Sentürk M Erdem D Küfrevioğlu Oİ Supuran CT 《Bioorganic & medicinal chemistry》2011,19(2):744-748
Carbonic anhydrase (CA; EC 4.2.1.1) was purified from the gill of the teleost fish Dicentrarchus labrax (European seabass). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The enzyme was purified 84.9-fold with a yield of 58%, and a specific activity of 838.9 U/mg proteins. It has an optimum pH at 8.0; an optimum temperature at 10°C. The kinetic parameters of this enzyme were determined for its esterase activity, with 4-nitrophenyl acetate (NPA) as substrate. The following anions, H?NSO??, I?, SCN?, NO??, NO??, N??, Br?, Cl?, SO?2?, and F? showed inhibitory effects on the enzyme. Sulfamic acid, iodide, and thiocyanate exhibited the strongest inhibitory action, in the micromolar range (K(i)s of 87-187 μM). NO??, NO?? and N?? were moderate inhibitors, whereas other anions showed only weak actions. All tested anions inhibited the enzyme in a competitive manner. Our findings indicate that these anions inhibit the fish enzyme in a similar manner to other α-CAs from mammals investigated earlier, but the susceptibility to various anions differs significantly between the fish and mammalian CAs. 相似文献
84.
Ozlem? Aksoy Feruzan?Dane Filiz?Ekinci Sanal Tulin?Aktac 《Acta Physiologiae Plantarum》2007,29(2):115-120
In this study, seed germination percentages, effects on phases of mitosis and α-amylase enzyme activity of lentil seeds treated
with four different concentrations (0.25, 0.5, 1 and 1.5%) of Fusilade (Fluazifop-p-butyl) were determined. Median EC (effective concentration) values were calculated according to seed germination percentages
after treatment for 72 h. Germination percentages of primary lentil roots decreased with increasing Fusilade concentrations.
Cytological observations showed that the mitotic frequency in root meristematic cells were decreased parallel to the increase
in concentrations and all Fusilade concentrations applied decreased the activity of α-amylase enzyme in lentil seeds. The
obtained results indicate that the herbicide Fusilade had the ability to cause reduction in seed germination, mitotic frequency
and also α-amylase activity of lentil seeds. 相似文献
85.
Turan Metin Ekinci Melek Kul Raziye Boynueyri Fatma G. Yildirim Ertan 《Journal of plant research》2022,135(3):517-529
Journal of Plant Research - This research hypothesized that tolerance of cucumber seedlings to salinity stress could be increased by hydrogen sulfide (H2S) treatments. In pot experiments, the... 相似文献
86.
Esma Kocaoğlu Hüseyin Çavdar Murat Şentürk Deniz Ekinci 《Journal of enzyme inhibition and medicinal chemistry》2019,34(1):51-54
Glutathione reductase (GR) is a crucial antioxidant enzyme which is responsible for the maintenance of antioxidant GSH molecule. Antimalarial effects of some chemical molecules are attributed to their inhibition of GR, thus inhibitors of this enzyme are expected to be promising candidates for the treatment of malaria. In this work, GR inhibitory properties of N-Methylpyrrole derivatives are reported. It was found that all compounds have better inhibitory activity than the strong GR inhibitor N,N-bis(2-chloroethyl)-N-nitrosourea, especially three molecules, 8?m, 8 n, and 8 q, were determined to be the most powerful among them. Findings of our study indicates that these Schiff base derivatives are strong GR inhibitors which can be used as leads for designation of novel antimalarial candidates. 相似文献
87.
Naiara Akizu Jennifer L. Silhavy Rasim Ozgur Rosti Eric Scott Ali G. Fenstermaker Jana Schroth Maha S. Zaki Henry Sanchez Neerja Gupta Madhulika Kabra Majdi Kara Tawfeg Ben-Omran Basak Rosti Alicia Guemez-Gamboa Emily Spencer Roger Pan Na Cai Mostafa Abdellateef Stacey Gabriel Jan Halbritter Friedhelm Hildebrandt Hans van Bokhoven Murat Gunel Joseph G. Gleeson 《American journal of human genetics》2014
88.
Julia Schreml Burak Durmaz Ozgur Cogulu Katharina Keupp Filippo Beleggia Esther Pohl Esther Milz Mahmut Coker Sema Kalkan Ucar Gudrun Nürnberg Peter Nürnberg Joachim Kuhn Ferda Ozkinay 《Human genetics》2014,133(1):29-39
Proteoglycan (PG) synthesis begins with the sequential addition of a “linker chain”, made up of four sugar residues, to a specific region of a core protein. Defects in the enzymes catalyzing steps two to four of the linker chain synthesis have been shown to cause autosomal recessive human phenotypes while no mutation has yet been reported in humans for the xylosyltransferases 1 and 2 (XT1 and XT2), the initiating enzymes in the linker chain formation. Here, we present a consanguineous Turkish family with two affected individuals presenting with short stature, distinct facial features, alterations of fat distribution, and moderate intellectual disability. X-rays showed only mild skeletal changes in the form of a short femoral neck, stocky and plump long bones and thickened ribs. Using a combination of whole-exome sequencing (WES), determination of homozygous stretches by WES variants, and classical linkage analysis, we identified the homozygous missense mutation c.C1441T in XYLT1, encoding XT1, within a large homozygous stretch on chromosome 16p13.12-p12.1. The mutation co-segregated with the phenotype in the family, is not found in over 13,000 alleles in the exome variant server and is predicted to change a highly conserved arginine at position 481 (p.R481W) located in the putative catalytical domain. Immunostaining of primary patient fibroblasts showed a loss of predominance of Golgi localization in mutant cells. Moreover, western blot analysis of decorin in cell culture supernatant demonstrated glycosylation differences between patient and control cells. Our data provide evidence that functional alterations of XT1 cause an autosomal recessive short stature syndrome associated with intellectual disability. 相似文献
89.
Hakan Erdem Zeliha Kocak-Tufan Omer Yilmaz Zuhal Karakurt Aykut Cilli Hulya Turkan Ozlem Yazicioglu-Mocin Nalan Adıguzel Gokay Gungor Canturk Taşcı Gulden Yilmaz Oral Oncul Aygul Dogan-Celik Ozcan Erdemli Nefise Oztoprak Yakup Tomak Asuman Inan Demet Tok Sibel Temur Hafize Oksuz Ozgur Senturk Unase Buyukkocak Fatma Yilmaz-Karadag Derya Ozturk-Engin Dilek Ozcengiz Ahmet Karakas Hayati Bilgic Hakan Leblebicioglu 《Annals of clinical microbiology and antimicrobials》2014,13(1):1-5
Background
Burkholderia cepacia complex (BCC) bacteria are highly virulent, typically multidrug-resistant, opportunistic pathogens in cystic fibrosis (CF) patients and other immunocompromised individuals. B. vietnamiensis is more often susceptible to aminoglycosides than other BCC species, and strains acquire aminoglycoside resistance during chronic CF infection and under tobramycin and azithromycin exposure in vitro, apparently from gain of antimicrobial efflux as determined through pump inhibition. The aims of the present study were to determine if oxidative stress could also induce aminoglycoside resistance and provide further observations in support of a role for antimicrobial efflux in aminoglycoside resistance in B. vietnamiensis.Findings
Here we identified hydrogen peroxide as an additional aminoglycoside resistance inducing agent in B. vietnamiensis. After antibiotic and hydrogen peroxide exposure, isolates accumulated significantly less [3H] gentamicin than the susceptible isolate from which they were derived. Strains that acquired aminoglycoside resistance during infection and after exposure to tobramycin or azithromycin overexpressed a putative resistance-nodulation-division (RND) transporter gene, amrB. Missense mutations in the repressor of amrB, amrR, were identified in isolates that acquired resistance during infection, and not in those generated in vitro.Conclusions
These data identify oxidative stress as an inducer of aminoglycoside resistance in B. vietnamiensis and further suggest that active efflux via a RND efflux system impairs aminoglycoside accumulation in clinical B. vietnamiensis strains that have acquired aminoglycoside resistance, and in those exposed to tobramycin and azithromycin, but not hydrogen peroxide, in vitro. Furthermore, the repressor AmrR is likely just one regulator of the putative AmrAB-OprM efflux system in B. vietnamiensis. 相似文献90.