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排序方式: 共有88条查询结果,搜索用时 31 毫秒
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Richard A Stabler Lisa F Dawson Petra CF Oyston Richard W Titball Jim Wade Jason Hinds Adam A Witney Brendan W Wren 《BMC microbiology》2008,8(1):177
Background
Human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. 相似文献54.
Egge-Jacobsen W Salomonsson EN Aas FE Forslund AL Winther-Larsen HC Maier J Macellaro A Kuoppa K Oyston PC Titball RW Thomas RM Forsberg Å Prior JL Koomey M 《Journal of bacteriology》2011,193(19):5487-5497
Findings from a number of studies suggest that the PilA pilin proteins may play an important role in the pathogenesis of disease caused by species within the genus Francisella. As such, a thorough understanding of PilA structure and chemistry is warranted. Here, we definitively identified the PglA protein-targeting oligosaccharyltransferase by virtue of its necessity for PilA glycosylation in Francisella tularensis and its sufficiency for PilA glycosylation in Escherichia coli. In addition, we used mass spectrometry to examine PilA affinity purified from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica and demonstrated that the protein undergoes multisite, O-linked glycosylation with a pentasaccharide of the structure HexNac-Hex-Hex-HexNac-HexNac. Further analyses revealed microheterogeneity related to forms of the pentasaccharide carrying unusual moieties linked to the distal sugar via a phosphate bridge. Type A and type B strains of Francisella subspecies thus express an O-linked protein glycosylation system utilizing core biosynthetic and assembly pathways conserved in other members of the proteobacteria. As PglA appears to be highly conserved in Francisella species, O-linked protein glycosylation may be a feature common to members of this genus. 相似文献
55.
Bernardo A Petriz Alinne P Castro Jeeser A Almeida Clarissa PC Gomes Gabriel R Fernandes Ricardo H Kruger Rinaldo W Pereira Octavio L Franco 《BMC genomics》2014,15(1)
Background
Obesity is a multifactor disease associated with cardiovascular disorders such as hypertension. Recently, gut microbiota was linked to obesity pathogenesisand shown to influence the host metabolism. Moreover, several factors such as host-genotype and life-style have been shown to modulate gut microbiota composition. Exercise is a well-known agent used for the treatment of numerous pathologies, such as obesity and hypertension; it has recently been demonstrated to shape gut microbiota consortia. Since exercise-altered microbiota could possibly improve the treatment of diseases related to dysfunctional microbiota, this study aimed to examine the effect of controlled exercise training on gut microbial composition in Obese rats (n = 3), non-obese Wistar rats (n = 3) and Spontaneously Hypertensive rats (n = 3). Pyrosequencing of 16S rRNA genes from fecal samples collected before and after exercise training was used for this purpose.Results
Exercise altered the composition and diversity of gut bacteria at genus level in all rat lineages. Allobaculum (Hypertensive rats), Pseudomonas and Lactobacillus (Obese rats) were shown to be enriched after exercise, while Streptococcus (Wistar rats), Aggregatibacter and Sutturella (Hypertensive rats) were more enhanced before exercise. A significant correlation was seen in the Clostridiaceae and Bacteroidaceae families and Oscillospira and Ruminococcus genera with blood lactate accumulation. Moreover, Wistar and Hypertensive rats were shown to share a similar microbiota composition, as opposed to Obese rats. Finally, Streptococcus alactolyticus, Bifidobacterium animalis, Ruminococcus gnavus, Aggregatibacter pneumotropica and Bifidobacterium pseudolongum were enriched in Obese rats.Conclusions
These data indicate that non-obese and hypertensive rats harbor a different gut microbiota from obese rats and that exercise training alters gut microbiota from an obese and hypertensive genotype background.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-511) contains supplementary material, which is available to authorized users. 相似文献56.
57.
Hobley G McKelvie JC Harmer JE Howe J Oyston PC Roach PL 《Bioorganic & medicinal chemistry letters》2012,22(9):3079-3082
A series of bisubstrate inhibitors for DNA N6 adenine methyltransferase (Dam) have been synthesized by linking an amine analogue of S-adenosylmethionine to an aryl moiety designed to probe the binding pocket of the DNA adenine base. An initial structure-activity relationship study has identified substituents that increase inhibitor potency to the ~10 μM range and improve selectivity against the human cytosine methyltransferase Dnmt1. 相似文献
58.
Eliana Alves Liliana Costa Carla MB Carvalho Jo?o PC Tomé Maria A Faustino Maria GPMS Neves Augusto C Tomé José AS Cavaleiro ?ngela Cunha Adelaide Almeida 《BMC microbiology》2009,9(1):70
Background
In recent times photodynamic antimicrobial therapy has been used to efficiently destroy Gram (+) and Gram (-) bacteria using cationic porphyrins as photosensitizers. There is an increasing interest in this approach, namely in the search of photosensitizers with adequate structural features for an efficient photoinactivation process. In this study we propose to compare the efficiency of seven cationic porphyrins differing in meso-substituent groups, charge number and charge distribution, on the photodynamic inactivation of a Gram (+) bacterium (Enterococcus faecalis) and of a Gram (-) bacterium (Escherichia coli). The present study complements our previous work on the search for photosensitizers that might be considered good candidates for the photoinactivation of a large spectrum of environmental microorganisms. 相似文献59.
Yersinia pestis pFra shows biovar-specific differences and recent common ancestry with a Salmonella enterica serovar Typhi plasmid 总被引:1,自引:0,他引:1
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Prentice MB James KD Parkhill J Baker SG Stevens K Simmonds MN Mungall KL Churcher C Oyston PC Titball RW Wren BW Wain J Pickard D Hien TT Farrar JJ Dougan G 《Journal of bacteriology》2001,183(8):2586-2594
Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have been a key element in this evolutionary leap from an enteric to a flea-transmitted systemic pathogen. However, the origin of Y. pestis-specific plasmids remains obscure. We demonstrate specific plasmid rearrangements in different Y. pestis strains which distinguish Y. pestis bv. Orientalis strains from other biovars. We also present evidence for plasmid-associated DNA exchange between Y. pestis and the exclusively human pathogen Salmonella enterica serovar Typhi. 相似文献
60.
Sialylation is a biosynthetic process occurring in the trans compartments
of the Golgi apparatus. Corresponding evidence is based on localization and
biochemical studies of alpha2, 6(N)-sialyltransferase (ST6Gal I) as
previously reported. Here we describe generation and characterization of
polyclonal antibodies to recombinant rat alpha2,3(N)-sialyltransferase
(ST3Gal III) expressed as a soluble enzyme in Sf9 cells or as a
beta-galactosidase-human-ST3Gal III fusion- protein from E.coli ,
respectively. These antibodies were used to localize ST3Gal III by
immunofluorescence in various cell lines and rat kidney tissue sections. In
transiently transfected COS cells the antibodies directed to soluble
sialyltransferase or the sialyltransferase portion of the fusion-protein
only recognized the recombinant antigen retained in the endoplasmic
reticulum. However, an antibody fraction crossreactive with
beta-galactosidase recognized natively expressed ST3Gal III which was found
to be colocalized with beta1, 4-galactosyltransferase in the Golgi
apparatus of several cultured cell lines. Antibodies affinity purified on
the beta- galactosidase-ST3Gal III fusion-protein column derived from both
antisera have then been used to localize the enzyme in perfusion-fixed rat
kidney sections. We found strong staining of the Golgi apparatus of tubular
epithelia and a brush-border-associated staining which colocalized with
cytochemical staining of the H+ATPase. This subcellular localization was
not observed for ST6Gal I which localized to the Golgi apparatus. These
data show colocalization in the Golgi apparatus and different post-Golgi
distributions of the two sialyltransferases.
相似文献