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991.
992.
We investigated variations in genetic diversity and plant fitness in a rare endemic metallophyte of calamine soils, Viola calaminaria, in relation to population size, population connectivity and population history in order to evaluate and discuss potential conservation strategies for the species. Mean population genetic diversity (H(s) = 0.25) of V. calaminaria was similar to endemic non-metallophyte taxa. Twenty-one per cent of the genetic variation was partitioned among populations and a low (9%) but significant differentiation was found among geographical regions. Our results did not support the hypothesis that the acquisition of metal tolerance may result in reduced genetic diversity, and suggested that strict metallophytes do not exhibit higher inter-population differentiation resulting from scattered habitats. There were no relationships between population genetic diversity and population size. Significant correlations were found between plant fitness and (i) population size and (ii) connectivity index. Recently-founded populations exhibited the same level of genetic diversity as ancient populations and also possessed higher plant fitness. There was no indication of strong founder effects in recently-established populations. The results suggest that the creation of habitats through human activities could provide new opportunities for conservation of this species. 相似文献
993.
994.
Complex regulation of the TRPC3, 6 and 7 channel subfamily by diacylglycerol and phosphatidylinositol-4,5-bisphosphate 总被引:4,自引:0,他引:4
TRPC3, 6 and 7 channels constitute a subgroup of non-selective, calcium-permeable cation channels within the TRP superfamily that are activated by products of phospholipase C-mediated breakdown of phosphatidylinositol-4,5-bisphosphate (PIP(2)). A number of ion channels, including other members of the TRP superfamily, are regulated directly by PIP(2). However, there is little information on the regulation of the TRPC channel subfamily by PIP(2). Pretreatment of TRPC7-expressing cells with a drug that blocks the synthesis of polyphosphoinositides inhibited the ability of the synthetic diacylglycerol, oleyl-acetyl glycerol, to activate TRPC7. In excised patches, TRPC7 channels were robustly activated by application of PIP(2) or ATP, but not by inositol 1,4,5-trisphosphate. Similar results were obtained with TRPC6 and TRPC3, although the effects of PIP(2) were somewhat less and with TRPC3 there was no significant effect of ATP. In the cell-attached configuration, TRPC7 channels could be activated by the synthetic diacylglycerol analog, oleyl-acetyl glycerol. However, this lipid mediator did not activate TRPC7 channels in excised patches. In addition, channel activation by PIP(2) in excised patches was significantly greater than that observed with oleyl-acetyl glycerol in the cell-attached configuration. These findings reveal complex regulation of TRPC channels by lipid mediators. The results also reveal for the first time direct activation by PIP(2) of members of the TRPC ion channel subfamily. 相似文献
995.
Derikx JP Matthijsen RA de Bruïne AP van Bijnen AA Heineman E van Dam RM Dejong CH Buurman WA 《PloS one》2008,3(10):e3428
Background
Intestinal ischemia-reperfusion (IR) is a phenomenon related to physiological conditions (e.g. exercise, stress) and to pathophysiological events (e.g. acute mesenteric ischemia, aortic surgery). Although intestinal IR has been studied extensively in animals, results remain inconclusive and data on human intestinal IR are scarce. Therefore, an experimental harmless model for human intestinal IR was developed, enabling us to clarify the sequelae of human intestinal IR for the first time.Methods and Findings
In 30 patients undergoing pancreatico-duodenectomy we took advantage of the fact that in this procedure a variable length of jejunum is removed. Isolated jejunum (5 cm) was subjected to 30 minutes ischemia followed by reperfusion. Intestinal Fatty Acid Binding Protein (I-FABP) arteriovenous concentration differences across the bowel segment were measured before and after ischemia to assess epithelial cell damage. Tissue sections were collected after ischemia and at 25, 60 and 120 minutes reperfusion and stained with H&E, and for I-FABP and the apoptosis marker M30. Bonferroni''s test was used to compare I-FABP differences. Mean (SEM) arteriovenous concentration gradients of I-FABP across the jejunum revealed rapidly developing epithelial cell damage. I-FABP release significantly increased from 290 (46) pg/ml before ischemia towards 3,997 (554) pg/ml immediately after ischemia (p<0.001) and declined gradually to 1,143 (237) pg/ml within 1 hour reperfusion (p<0.001). Directly after ischemia the intestinal epithelial lining was microscopically normal, while subepithelial spaces appeared at the villus tip. However, after 25 minutes reperfusion, enterocyte M30 immunostaining was observed at the villus tip accompanied by shedding of mature enterocytes into the lumen and loss of I-FABP staining. Interestingly, within 60 minutes reperfusion the epithelial barrier resealed, while debris of apoptotic, shedded epithelial cells was observed in the lumen. At the same time, M30 immunoreactivity was absent in intact epithelial lining.Conclusions
This is the first human study to clarify intestinal IR induced cell damage and repair and its direct consequences. It reveals a unique, endogenous clearing mechanism for injured enterocytes: rapid detachment of damaged apoptotic enterocytes into the lumen. This process is followed by repair of the epithelial continuity within an hour, resulting in a normal epithelial lining. 相似文献996.
Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells 总被引:1,自引:0,他引:1
Berdasco M Alcázar R García-Ortiz MV Ballestar E Fernández AF Roldán-Arjona T Tiburcio AF Altabella T Buisine N Quesneville H Baudry A Lepiniec L Alaminos M Rodríguez R Lloyd A Colot V Bender J Canal MJ Esteller M Fraga MF 《PloS one》2008,3(10):e3306
Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells. 相似文献
997.
Willis ND Cox TR Rahman-Casañs SF Smits K Przyborski SA van den Brandt P van Engeland M Weijenberg M Wilson RG de Bruïne A Hutchison CJ 《PloS one》2008,3(8):e2988
Background
A-type lamins are type V intermediate filament proteins encoded by the gene LMNA. Mutations in LMNA give rise to diverse degenerative diseases related to premature ageing. A-type lamins also influence the activity of the Retinoblastoma protein (pRb) and oncogenes such a β-catenin. Consequently, it has been speculated that expression of A-type lamins may also influence tumour progression.Methodology/Principal Findings
An archive of colorectal cancer (CRC) and normal colon tissue was screened for expression of A-type lamins. We used the Cox proportional hazard ratio (HR) method to investigate patient survival. Using CRC cell lines we investigated the effects of lamin A expression on other genes by RT-PCR; on cell growth by FACS analysis; and on invasiveness by cell migration assays and siRNA knockdown of targeted genes. We found that lamin A is expressed in colonic stem cells and that patients with A-type lamin-expressing tumours have significantly worse prognosis than patients with A-type lamin negative tumours (HR = 1.85, p = 0.005). To understand this finding, we established a model system based upon expression of GFP-lamin A in CRC cells. We found that expression of GFP-lamin A in these cells did not affect cell proliferation but did promote greatly increased cell motility and invasiveness. The reason for this increased invasiveness was that expression of lamin A promoted up-regulation of the actin bundling protein T-plastin, leading to down regulation of the cell adhesion molecule E-cadherin.Conclusions
Expression of A-type lamins increases the risk of death from CRC because its presence gives rise to increased invasiveness and potentially a more stem cell-like phenotype. This report directly links A-type lamin expression to tumour progression and raises the profile of LMNA from one implicated in multiple but rare genetic conditions to a gene involved in one of the commonest diseases in the Western World. 相似文献998.
Mohammed Ramdani Najat Elkhiati Roger J. Flower Hilary H. Birks Mejdidine M. Kraïem Adel A. Fathi Simon T. Patrick 《Aquatic Ecology》2001,35(3-4):319-333
Zooplankton (Copepoda, Cladocera, Ostracoda, Rotifera and Diptera larvae) in nine North African lakes was collected from open water areas over twenty months during 1997/99. The results were used to monitor changes in the pelagic micro-invertebrate fauna of these sites with the purpose of exploring diversity structure and regional species occurrences.The studied sites formed three distinct groups based on hydrology and water quality criteria: (i) acid water with no marine connection (Megene Chitane); (ii) alkaline freshwater/brackish with no marine connection (Merja Sidi Bou Rhaba and Merja Bokka); (iii) freshwater/brackish with marine connection (Merja Zerga, Lac de Korba, Garaet El Ichkeul and three Nile Delta lakes). However, cluster analysis of the zooplankton data alone indicated four groups with Korba being separated because of its prevalence of species tolerant of summer hypersalinity.The total regional zooplanktonic species richness found was 88 taxa and these were characterized by species tolerant of widely fluctuating environmental conditions. However, some recorded species were very rare for North African freshwaters (e.g. Alonella excisa, Leydigia quadrangularis and, Ilyocryptus sordidus) and generally indicate favourable environmental conditions of low salinity and temperature. The sites influenced by marine waters generally exhibited slightly lower numbers of species but which generally demonstrate cosmopolitan distributions. Distinct seasonal patterns in species distributions were more similar to those observed in European lakes rather than to those of lower latitudes sites.Zooplankton play a key role in maintaining aquatic ecosystem quality in the North African study lakes and the community distributions described for the late 20th century help set biodiversity base-line data for future studies. If the remaining wetland lakes in this region are to persist as important resources during the 21st century, they will need to be managed in a way that ensures that aquatic diversity is maintained. 相似文献
999.
Radiometrically determined dates and sedimentation rates for recent sediments in nine North African wetland lakes (the CASSARINA Project) 总被引:3,自引:0,他引:3
P.G. Appleby H.H. Birks R.J. Flower N. Rose S.M. Peglar M. Ramdani M.M. Kraïem A.A. Fathi 《Aquatic Ecology》2001,35(3-4):347-367
Sediment cores were collected from nine wetland lakes in Morocco, Tunisia and Egypt for the CASSARINA project investigating environmental change in Northern African wetlands. The cores were dated radiometrically by using natural (210Pb) and artificial (137Cs and 241Am) radionuclides. At sites in Morocco and Tunisia with mean annual rainfall totals ranging from 500–1000 mm yr–1, fallout records were generally satisfactory and it was possible to develop independent sediment chronologies based on the radiometric data alone. At the Egyptian sites, rainfall was less than 200 mm yr–1 and fallout records were much less distinct. At these sites the radiometric data could only be used to give an indication of mean sedimentation rates during the past 30–40 years. By using a combination of fallout radionuclide, pollen, and macrofossil stratigraphic records it was however possible to determine a credible sediment chronology spanning the major part of the 20th century. Applying this chronology to records of spheroidal carbonaceous particles (SCP) from the same sediment cores, the onset of significant levels of atmospheric pollution in the Nile Delta is dated in all three cores to the mid 1950s. Results from a number of lakes (Sidi Bou Rhaba, Ichkeul and Korba) revealed high and accelerating siltation rates, threatening their continued existence beyond the next few decades. In contrast, sedimentation rates at all three Nile Delta sites appear to have declined in recent decades, most probably due to the impact of the Nile barrages. 相似文献
1000.
Terrestrial pollen record of recent land-use changes around nine North African lakes in the CASSARINA Project 总被引:1,自引:0,他引:1
Sylvia M. Peglar Hilary H. Birks H. J. B. Birks A.A. Fathi R.J. Flower M.M. Kraïem S.T. Patrick M. Ramdani 《Aquatic Ecology》2001,35(3-4):431-448
Pollen analyses and related plant macrofossil records are presented from short cores from nine North African lakes in the EU-funded CASSARINA project. Terrestrial pollen reflects human impact on the vegetation and landscape over the last 150–100 years. Pollen changes, aided by radiometric dating, could be correlated with historical developments. The chronology of the landscape changes date other biostratigraphical records reflecting changes in the aquatic ecosystems.Three lakes in Morocco show gradually intensifying land-use over the last century. Accelerated technological development and landscape modification over the last 20 years culminated in one of the lakes being drained and cultivated during the project period. In Tunisia, a nationally unique acid-water lake is threatened by water withdrawal for increased catchment cultivation. The landscape around two other lakes is being increasingly cultivated and urbanised, and water withdrawal to support this has resulted in deleterious effects on the aquatic ecosystems, particularly at the internationally famous Garaet El Ichkeul where reed-marshes and macrophyte beds have been lost. The three lakes in the Egyptian Nile Delta are in the same hydrological system and show parallel changes in the balance between saltmarsh and reed-marsh. Control of Nile floods and year-round irrigation led to marked increases in cultivation in the delta region since ca. 1920. The Aswan High Dam (1964) had little detectable further effect. Documented planting of dates, palms, and olives and of introduced Casuarina and Eucalyptus trees provided a chronology to supplement the unsatisfactory radiometric dating of the sediments in this low-rainfall area. 相似文献