Die Histiogenese der Planarienregenerate

Ohne Zusammenfassung     

Die systematische Stellung vonTaiwania cryptomerioides Hayata

Ohne ZusammenfassungVor allem bin ich meinem verehrten Lehrer, Herrn Hofrat Prof. Dr. R. Wettstein, der mich zu dieser Arbeit angeregt hat, zu großem Dank verpflichtet. Außerdem danke ich Herrn Kustos Dr. H. Handel-Mazzetti für die Überlassung des von ihm in China gesammelten Materiales, ferner den Herren Prof. Dr. F. Knoll, Dozent Dr. B. Schussnig, Assistent Dr. H. Neumayer und Assistent Dr. H. Brunswik, die meine Arbeit durch Beschaffung von Vergleichsmaterial und durch technische Eatschläge gefördert haben.     

TEMPERATURE AND FORWARD MOVEMENT OF PARAMECIUM

1. The rate of forward movement in Paramecium as affected by changes in temperature can be described accurately in terms of the Arrhenius equation. See PDF for Equation 2. For the range from 6–15°, µ = 16,000; from 16–40°, µ = 8,000. These values fall within the limits characteristic for chemical processes. 3. On the principle of velocity control by the slowest rate, it is assumed that in Paramecium at temperatures above normal, control passes from one underlying reaction to another. 4. The views expressed by Rice, the recent results of Crozier, and certain µ values given by Arrhenius all suggest that µ = 16,000 may represent an oxidation, and µ = 8,000 either a modified oxidation or an hydrolysis. 5. For the system of controls, the catenary series O → A → E with the lower µ value attached to the precursor reaction is adequate. We may also assume a cyclical system analogous to Meyerhof''s conception of carbohydrate metabolism in muscle. In this case it is necessary to assign µ = 16,000 to the oxidation of A and E and µ = 8,000 to the synthesis E → O. This model also accounts for the fact that the data might be interpreted as involving, apparently, a depletion of A at the higher temperature.     

Purification and characterization of an 85 kDa talin-binding fragment of vinculin

Vinculin and talin are adhesion plaque proteins which have been shown to interact with each other in vitro. In order to begin to investigate where the talin-binding domain is in vinculin, vinculin was digested with Staphylococcus aureus V8 protease to generate two major fragments of 85 and 30 kDa, and these fragments were purified. Nitrocellulose overlays with 125I-talin and the 125I-85 kDa vinculin fragment and sucrose density gradient centrifugation demonstrated that the talin-binding domain was localized to the 85 kDa vinculin fragment. Quantification of 125I-talin binding in the overlays showed that four times more talin bound to the 85 kDa fragment as compared to intact vinculin. Competitive immunoprecipitation experiments demonstrated that unlabeled 85 kDa fragment was about three-fold more effective at competing for 125I-85 kDa binding to talin than was unlabeled vinculin. These results suggest that the 30 kDa fragment inhibits the vinculin-talin interaction even though the talin-binding domain is localized in the 85 kDa fragment.     

Prinzipien und Beispiele der Anwendung phytoserologischer Methodik

Ohne ZusammenfassungMit 13 Textabbildungen.