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281.
Inter-annual variation in the soil seed bank of flood-meadows over two years with different flooding patterns 总被引:3,自引:0,他引:3
Persistent soil seed banks are presumed to play a significant role in bridging and exploiting the effects of major flood disturbances
in riparian systems. However, presently there is little empirical data on the correlation between flooding and seed bank dynamics.
The objective of this study was to assess the impact of inter-annual flooding variation on soil seed bank dynamics in flood-meadows.
We analysed seed bank composition at 46 plots along the northern Upper Rhine River, Germany, after two periods with contrasting
flooding conditions (1996–1997: low and rare flooding; 1998–1999 high and very frequent flooding). Between both sampling occasions
the total number of seedlings emerging from the seed bank decreased by 50% and average species-richness per sample by 30%,
irrespective of the sampling depth. Similar decline occurred in mesophytes and wetland species as well as over different functional
groups, with the exception of legumes. Considerable compositional changes in the seed bank were also indicated by shifts in
DCA ordination and by the comparison of similarity matrices from both years with the Mantel test. The Mantel test also confirmed
that compositional changes were more pronounced in the seed bank than in aboveground vegetation. There was poor correlation
between the decline in total seed densities and species in the soil seed bank and environmental variables such as flooding
duration and ecological groupings such as floodplain compartment and seed bank community types. Further distinct patterns
of decline and persistence were evident only at the species level. While 21 species displayed a significant decline, only
two species increased. Annual arable weeds and perennial ruderals with high temperature or nutrient requirements to break
dark dormancy were amongst the most significantly declining species. In contrast, there was no decline in typical winter annuals
and certain perennials that preferably germinate in autumn and fall into dormancy at the beginning of the warm period. Generally,
differences in germination requirements and dormancy cycles of species gave the best explanation for the patterns of decline
and persistence after intense flooding. Given these findings, the heavy depletion of the persistent soil seed bank we observed
is most likely attributed to a post-flood germination flush due to very favourable recruitment conditions prevailing during
the draw-down period after early summer flooding in 1999. The results of our study suggest that persistent soil seed banks
are of outstanding importance to bridge and exploit the highly variable hydrological conditions that are typical of flood-meadows.
Thus, they play a significant role in maintaining the floristic diversity of this habitat type over time and space.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Christiaan H. Vinkers Herm J. Lamberink Joeri K. Tijdink Pauline Heus Lex Bouter Paul Glasziou David Moher Johanna A. Damen Lotty Hooft Willem M. Otte 《PLoS biology》2021,19(4)
Many randomized controlled trials (RCTs) are biased and difficult to reproduce due to methodological flaws and poor reporting. There is increasing attention for responsible research practices and implementation of reporting guidelines, but whether these efforts have improved the methodological quality of RCTs (e.g., lower risk of bias) is unknown. We, therefore, mapped risk-of-bias trends over time in RCT publications in relation to journal and author characteristics. Meta-information of 176,620 RCTs published between 1966 and 2018 was extracted. The risk-of-bias probability (random sequence generation, allocation concealment, blinding of patients/personnel, and blinding of outcome assessment) was assessed using a risk-of-bias machine learning tool. This tool was simultaneously validated using 63,327 human risk-of-bias assessments obtained from 17,394 RCTs evaluated in the Cochrane Database of Systematic Reviews (CDSR). Moreover, RCT registration and CONSORT Statement reporting were assessed using automated searches. Publication characteristics included the number of authors, journal impact factor (JIF), and medical discipline. The annual number of published RCTs substantially increased over 4 decades, accompanied by increases in authors (5.2 to 7.8) and institutions (2.9 to 4.8). The risk of bias remained present in most RCTs but decreased over time for allocation concealment (63% to 51%), random sequence generation (57% to 36%), and blinding of outcome assessment (58% to 52%). Trial registration (37% to 47%) and the use of the CONSORT Statement (1% to 20%) also rapidly increased. In journals with a higher impact factor (>10), the risk of bias was consistently lower with higher levels of RCT registration and the use of the CONSORT Statement. Automated risk-of-bias predictions had accuracies above 70% for allocation concealment (70.7%), random sequence generation (72.1%), and blinding of patients/personnel (79.8%), but not for blinding of outcome assessment (62.7%). In conclusion, the likelihood of bias in RCTs has generally decreased over the last decades. This optimistic trend may be driven by increased knowledge augmented by mandatory trial registration and more stringent reporting guidelines and journal requirements. Nevertheless, relatively high probabilities of bias remain, particularly in journals with lower impact factors. This emphasizes that further improvement of RCT registration, conduct, and reporting is still urgently needed.Many randomized controlled trials (RCTs) are biased and difficult to reproduce due to methodological flaws and poor reporting. Analysis of 176,620 RCTs published between 1966 and 2018 reveals that the risk of bias in RCTs generally decreased. Nevertheless, relatively high probabilities of bias remain, showing that further improvement of RCT registration, conduct, and reporting is still urgently needed. 相似文献
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Expression and potential functions of G-protein alpha subunits in embryos of Xenopus laevis. 总被引:1,自引:0,他引:1
A P Otte L L McGrew J Olate N M Nathanson R T Moon 《Development (Cambridge, England)》1992,116(1):141-146
During early embryonic development, many inductive interactions between tissues depend on signal transduction processes. We began to test the possibility that G-proteins participate in the signal transduction pathways that mediate neural induction. The expression during Xenopus development of three G alpha subunits, G alpha 0, G alpha i-1 and G alpha s-1, was characterized. The three maternally expressed genes showed different expression patterns during early development. Whole-mount in situ hybridization revealed that all three genes were expressed almost exclusively in the gastrula ectoderm and predominantly in the neuroectoderm in the neurula embryo. In order to investigate the involvement of these proteins in neural induction, we overexpressed the G-protein alpha subunits by injecting the G alpha mRNAs into fertilized eggs. Overexpression of G alpha s-1 increased the ability of gastrula ectoderm to become induced to neural tissue approximately four-fold. Overexpression of G alpha 0 and G alpha i-1 had less pronounced effects on neural competence, and inhibition of the G alpha 0 and G alpha i-1 proteins by pertussis toxin did not change the neural competence of the exposed gastrula ectoderm. Overexpression of the G alpha 0 and G alpha i-1 genes did, however, inhibit the normal disappearance of the blastocoel during gastrulation, suggesting a role for these G-proteins in regulating this process. The data also suggest a specific role for the G alpha s subunit in mediating the initial phases of neural induction. 相似文献
287.
In rare plants that often occur in small or isolated populations the probability of selfing between close relatives is increased as a consequence of demographic stochasticity. The mode of pollination (selfing, outcrossing) may have considerable effects on seed traits and offspring performance and hence potential viability. Since current efforts aiming at the restoration of floodplain grasslands through the transfer of plant material from species-rich source stands may lead to the establishment of initially small populations consisting of founders from different populations, the present paper experimentally investigated the effects of pollen source and floral types (i.e. chasmogamous (CH) and cleistogamous (CL) flowers) on seed traits and offspring performance in three highly endangered violet species (Viola elatior, V. pumila, V. stagnina) of these grasslands. We estimated inbreeding depression and tested the performance of selfed and outcrossed offspring in two microbial environments, i.e. in soil inoculated with (i) non-sterile substrate from the same species (‘home’-conditions) and (ii) sterilised substrate.Plants produced more CL capsules than CH flowers. Pollinator exclusion had only small effects on CH seed production. CL seeds had a significantly lower mass per seed than CH seeds. This may be related to constraints in allocation or environmental conditions. Seedling growth was reduced in plants grown under ‘home’-conditions as compared to control soils. Under ‘home’-conditions, relative fitness of selfed seedlings of V. stagnina was significantly higher than that of crossed progeny. Our results suggest that high genetic differentiation among populations as a consequence of isolation may result in outbreeding depression, e.g., through biochemical or physiological incompatibilities between genes or the breaking of coadapted gene complexes. In V. stagnina, offspring fitness differed considerably between environments, but in general we found no indications for inbreeding depression in these rare species. 相似文献
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The restricted ability of embryonic tissue to respond to inductive signals is controlled by a poorly understood phenomenon, termed competence. In Xenopus, dorsal ectoderm is more competent than ventral ectoderm to become induced to neural tissue. We tested whether the Xenopus protein kinase C (PKC) isozymes alpha and beta have a role in neural induction and competence. We found that PKC alpha is predominantly localized in dorsal ectoderm, whereas PKC beta is uniformly distributed. Overexpression of PKC beta conveys a higher propensity for neural differentiation to both dorsal and ventral ectoderm, but their difference in competence remains. However, ectopic expression of PKC alpha elevates the level of neural competence of ventral ectoderm to that of dorsal ectoderm. These data indicate that different PKC isozymes have distinct roles in mediating both neural induction and competence. 相似文献