全文获取类型
收费全文 | 778篇 |
免费 | 38篇 |
出版年
2022年 | 8篇 |
2021年 | 15篇 |
2019年 | 6篇 |
2018年 | 11篇 |
2017年 | 8篇 |
2016年 | 24篇 |
2015年 | 23篇 |
2014年 | 14篇 |
2013年 | 48篇 |
2012年 | 44篇 |
2011年 | 37篇 |
2010年 | 22篇 |
2009年 | 12篇 |
2008年 | 34篇 |
2007年 | 22篇 |
2006年 | 35篇 |
2005年 | 23篇 |
2004年 | 22篇 |
2003年 | 34篇 |
2002年 | 31篇 |
2001年 | 27篇 |
2000年 | 27篇 |
1999年 | 20篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1992年 | 15篇 |
1991年 | 11篇 |
1990年 | 16篇 |
1989年 | 8篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 10篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 11篇 |
1974年 | 10篇 |
1972年 | 13篇 |
1971年 | 10篇 |
1968年 | 11篇 |
1967年 | 7篇 |
1966年 | 4篇 |
1965年 | 4篇 |
排序方式: 共有816条查询结果,搜索用时 953 毫秒
61.
Halberg F Cornélissen G Stoynev A Ikonomov O Katinas G Sampson M Wang Z Wan C Singh RB Otsuka K Sothern RB Sothern SB Sothern MI Syutkina EV Masalov A Perfetto F Tarquini R Maggioni C Kumagai Y Siegelova J Fiser B Homolka P Dusek J Uezono K Watanabe Y Wu J Prikryl P Blank M Blank O Sonkowsky R Schwartzkopff O Hellbrügge T Spector NH Baciu I Hriscu M Bakken E 《Neuro endocrinology letters》2003,24(6):479-498
62.
Ogura T Morita K Takeuchi M Otsuka F Iwasaki T Oe K Matsuura K Tobe K Mimura Y Kishida M Makino H Hirakawa M 《Hormone research》2003,59(1):30-34
AIM: The roles of adrenomedullin (AM) in body fluid balance under general anesthesia were investigated. METHODS: Time course changes in plasma osmolality, AM, arginine vasopressin (AVP), and urinary aquaporin 2 (AQP2) in 17 patients undergoing abdominal surgery under general anesthesia were examined. RESULTS: Increases in plasma AM levels were observed in parallel with increases in the levels of urinary AQP2/creatinine (Cr) before induction and 90 and 180 min after initiation of anesthesia. Significant correlations between plasma AM and urinary AQP2/Cr (r = 0.62, p < 0.0001) as well as urinary AVP/Cr and AQP2/Cr (r = 0.60, p < 0.0001) were uncovered. Multivariate stepwise analysis identified plasma AM as the critical independent factor affecting urinary AQP2/Cr level. CONCLUSION: A novel correlation of AM and AQP2 which overlays an AVP-AQP2 system may play a key role in fluid homeostasis during general anesthesia. 相似文献
63.
Activity-dependent mRNA splicing controls ER export and synaptic delivery of NMDA receptors 总被引:12,自引:0,他引:12
Activity-dependent targeting of NMDA receptors (NMDARs) is a key feature of synapse formation and plasticity. Although mechanisms for rapid trafficking of glutamate receptors have been identified, the molecular events underlying chronic accumulation or loss of synaptic NMDARs have remained unclear. Here we demonstrate that activity controls NMDAR synaptic accumulation by regulating forward trafficking at the endoplasmic reticulum (ER). ER export is accelerated by the alternatively spliced C2' domain of the NR1 subunit and slowed by the C2 splice cassette. This mRNA splicing event at the C2/C2' site is activity dependent, with C2' variants predominating upon activity blockade and C2 variants abundant with increased activity. The switch to C2' accelerates NMDAR forward trafficking by enhancing recruitment of nascent NMDARs to ER exit sites via binding of a divaline motif within C2' to COPII coats. These results define a novel pathway underlying activity-dependent targeting of glutamate receptors, providing an unexpected mechanistic link between activity, mRNA splicing, and membrane trafficking during excitatory synapse modification. 相似文献
64.
Hamasaki A Naka H Tamanoi F Umezawa K Otsuka M 《Bioorganic & medicinal chemistry letters》2003,13(9):1523-1526
A novel metal chelator comprising a 4-(naphthalen-1-yl)pyridine and 2-aminoethanethiol was synthesized. This showed inhibitory activity against human protein farnesyltransferase with IC(50) 1.9 microM, induced morphological change in K-ras-NRK cells at 0.5 microg/mL and showed growth inhibition of K-ras-NRK cells with IC(50) 0.32 microg/mL. 相似文献
65.
2',5'-isoDNA mimic chiral peptide nucleic acid (isoPNA) monomers derived from D- and L-aspartic acids were synthesized. These novel monomers were incorporated in aminoethylglycine peptide nucleic acid (aegPNA) thymine dodecamers, and the hybridization properties to RNA and DNA were demonstrated by UV thermal denaturation. 相似文献
66.
67.
The Caenorhabditis elegans nuc-1 gene has previously been implicated in programmed cell death due to the presence of persistent undegraded apoptotic DNA in nuc-1 mutant animals. In this report, we describe the cloning and characterization of nuc-1, which encodes an acidic nuclease with significant sequence similarity to mammalian DNase II. Database searches performed with human DNase II protein sequence revealed a significant similarity with the predicted C. elegans C07B5.5 ORF. Subsequent analysis of crude C. elegans protein extracts revealed that wild-type animals contained a potent endonuclease activity with a cleavage preference similar to DNase II, while nuc-1 mutant worms demonstrated a marked reduction in this nuclease activity. Sequence analysis of C07B5.5 DNA and mRNA also revealed that nuc-1(e1392), but not wild-type animals contained a nonsense mutation within the CO7B5.5 coding region. Furthermore, nuc-1 transgenic lines carrying the wild-type C07B5.5 locus demonstrated a complete complementation of the nuc-1 mutant phenotype. Our results therefore provide compelling evidence that the C07B5.5 gene encodes the NUC-1 apoptotic nuclease and that this nuclease is related in sequence and activity to DNase II. 相似文献
68.
Takeda Y Matsumura H Masuda T Honda G Otsuka H Takaishi Y Sezik E Yesilada E 《Phytochemistry》2000,53(8):931-935
From the aerial parts of Phlomis rigida, three iridoid glucosides, phlorigidoside A (2-O-acetyllamiridoside), B (8-O-acetyl-6-beta-hydroxyipolamide) and C (5-deoxysesamoside), were isolated together with the known iridoid glucosides, shanzhiside methyl ester, 8-O-acetylshanzhiside methyl ester, deoxypulcheloside I, lamiridoside, and 6-beta-hydroxyipolamide. The structures of the new compounds were elucidated based on spectral and chemical evidence. 相似文献
69.
At the present stage, many papers on MEKC, which include fundamental characteristics and applications, have been available. Because only brief discussion on some aspects of MEKC was described in this article, it is necessary to refer some of those literature when the detailed information is required. Especially, for optimization strategies of MEKC, which was not discussed in this article, theoretical discussions by Foley(85), Vindevogel and Sandra(86), and Khaledi and coworkers(87,88) should be cited, along with the review article(11). 相似文献
70.