全文获取类型
收费全文 | 370篇 |
免费 | 35篇 |
出版年
2023年 | 3篇 |
2022年 | 6篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 10篇 |
2018年 | 8篇 |
2017年 | 12篇 |
2016年 | 18篇 |
2015年 | 17篇 |
2014年 | 27篇 |
2013年 | 31篇 |
2012年 | 27篇 |
2011年 | 28篇 |
2010年 | 23篇 |
2009年 | 23篇 |
2008年 | 16篇 |
2007年 | 12篇 |
2006年 | 20篇 |
2005年 | 17篇 |
2004年 | 9篇 |
2003年 | 17篇 |
2002年 | 15篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1966年 | 2篇 |
1964年 | 1篇 |
1963年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有405条查询结果,搜索用时 0 毫秒
401.
Ruth PercinoDaniel Jos M. Contreras Lpez Oswaldo TllezValds Fausto R. Mndez de la Cruz Alejandro GonzalezVoyer Daniel Piero 《Ecology and evolution》2021,11(11):6688
- Tropical ectotherm species tend to have narrower physiological limits than species from temperate areas. As a consequence, tropical species are considered highly vulnerable to climate change since minor temperature increases can push them beyond their physiological thermal tolerance. Differences in physiological tolerances can also be seen at finer evolutionary scales, such as among populations of ectotherm species along elevation gradients, highlighting the physiological sensitivity of such organisms.
- Here, we analyze the influence of elevation and bioclimatic domains, defined by temperature and precipitation, on thermal sensitivities of a terrestrial direct‐developing frog (Craugastor loki) in a tropical gradient. We address the following questions: (a) Does preferred temperature vary with elevation and among bioclimatic domains? (b) Do thermal tolerance limits, that is, critical thermal maximum and critical thermal minimum vary with elevation and bioclimatic domains? and (c) Are populations from high elevations more vulnerable to climate warming?
- We found that along an elevation gradient body temperature decreases as environmental temperature increases. The preferred temperature tends to moderately increase with elevation within the sampled bioclimatic domains. Our results indicate that the ideal thermal landscape for this species is located at midelevations, where the thermal accuracy (db) and thermal quality of the environment (de) are suitable. The critical thermal maximum is variable across elevations and among the bioclimatic domains, decreasing as elevation increases. Conversely, the critical thermal minimum is not as variable as the critical thermal maximum.
- Populations from the lowlands may be more vulnerable to future increases in temperature. We highlight that the critical thermal maximum is related to high temperatures exhibited across the elevation gradient and within each bioclimatic domain; therefore, it is a response to high environmental temperatures.
402.
The ultrastructure of spermatid development during spermiogenesis within the rosebelly lizard,Sceloporus variabilis (Reptilia,Squamata, Phrynosomatidae) 下载免费PDF全文
Kevin M. Gribbins Caroline L. Matchett Kathryn A. DelBello Justin Rheubert Maricela Villagrán‐SantaCruz Gisela Granados‐González Oswaldo Hernández‐Gallegos 《Journal of morphology》2014,275(3):258-268
Several recent studies have mapped out the characters of spermiogenesis within several species of squamates. Many of these data have shown both conserved and possibly apomorphic morphological traits that could be important in future phylogenetic analysis within Reptilia. There, however, has not been a recent study that compares spermiogenesis and its similarities or differences between two species of reptile that reside in the same genus. Thus, the present analysis details the changes to spermiogenesis in Sceloporus variabilis and then compares spermatid morphologies to that of Sceloporus bicanthalis. Many of the morphological changes that the spermatids undergo in these two species are similar or conserved, which is similar to what has been reported in other squamates. There are six main character differences that can be observed during the development of the spermatids between these two sceloporid lizards. They include the presence (S. variabilis) or absence (S. bicanthalis) of a mitochondrial/endoplasmic reticulum complex near the Golgi apparatus during acrosome development, a shallow (S. variabilis) or deep (S. bicanthalis) nuclear indentation that accommodates the acrosomal vesicle, filamentous (S. variabilis) or granular (S. bicanthalis) chromatin condensation, no spiraling (S. variabilis) or spiraling (S. bicanthalis) of chromatin during condensation, absence (S. variabilis) or presence (S. bicanthalis) of the longitudinal manchette microtubules, and the lack of (S. variabilis) or presence (S. bicanthalis) of nuclear lacunae. This is the first study that compares spermiogenic ultrastructural characters between species within the same genus. The significance of the six character differences between two distantly related species within Sceloporus is still unknown, but these data do suggest that spermiogenesis might be a good model to study the hypothesis that spermatid ontogeny is species specific. J. Morphol. 275:258–268, 2014. © 2013 Wiley Periodicals, Inc. 相似文献
403.
Enhanced Nitrogen Fixation in a Rhizobium etli ntrC Mutant That Overproduces the Bradyrhizobium japonicum Symbiotic Terminal Oxidase cbb3 总被引:1,自引:0,他引:1 下载免费PDF全文
404.
405.
Nicolas Oswaldo Gomez Alexandre Tetard Laurent Ouerdane Clmentine Laffont Catherine Brutesco Genevive Ball Ryszard Lobinski Yann Denis Patrick Plsiat Catherine Llanes Pascal Arnoux Rom Voulhoux 《Molecular microbiology》2021,115(1):84-98
To overcome the metal restriction imposed by the host’s nutritional immunity, pathogenic bacteria use high metal affinity molecules called metallophores. Metallophore‐mediated metal uptake pathways necessitate complex cycles of synthesis, secretion, and recovery of the metallophore across the bacterial envelope. We recently discovered staphylopine and pseudopaline, two members of a new family of broad‐spectrum metallophores important for bacterial survival during infections. Here, we are expending the molecular understanding of the pseudopaline transport cycle across the diderm envelope of the Gram‐negative bacterium Pseudomonas aeruginosa. We first explored pseudopaline secretion by performing in vivo quantifications in various genetic backgrounds and revealed the specific involvement of the MexAB–OprM efflux pump in pseudopaline transport across the outer membrane. We then addressed the recovery part of the cycle by investigating the fate of the recaptured metal‐loaded pseudopaline. To do so, we combined in vitro reconstitution experiments and in vivo phenotyping in absence of pseudopaline transporters to reveal the existence of a pseudopaline modification mechanism, possibly involved in the metal release following pseudopaline recovery. Overall, our data allowed us to provide an improved molecular model of secretion, recovery, and fate of this important metallophore by P. aeruginosa. 相似文献