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101.
Matías García Azucena Gonzalez-Coloma Osvaldo J. Donadel Carlos E. Ardanaz Carlos E. Tonn Marta E. Sosa 《Biochemical Systematics and Ecology》2007
Flourensia oolepis Blake (Asteraceae) essential oil had a complex chemical composition with τ-muurolene (6.14%), santolinetriene (6.22%), 2-methylene-4,8,8-trimethyl-4-vinyl-bicyclo[5.2.0]nonane (10.15%), δ-cadinene (10.27%) and γ-gurjunene (20.69%) comprising more than 50% of the oil. This oil had repellent and toxic effects on Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) adults, acting as a contact toxin. Myzus persicae (Sulzer) (Homoptera: Aphididae) and Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) adults showed behavioral sensibility to this oil. 相似文献
102.
Zavan B Giorgi C Bagnara GP Vindigni V Abatangelo G Cortivo R 《European journal of histochemistry : EJH》2007,51(Z1):1-8
Hyaluronan-based scaffold were used for in vitro commitment of human and rat bone marrow mesenchymal stem cells (MSC). Cells were cultured either in monolayer and in 3D conditions up to 35 days. In order to monitor the differentiating processes molecular biology and morphological studies were performed at different time points. All the reported data supported the evidence that both human and rat MSC grown onto hyaluronan-derived three-dimensional scaffold were able to acquire a unique phenotype of chondrocytes and osteocytes depending on the presence of specific differentiation inducing factors added into the culture medium without significative differences in term of time expression of extracellular matrix proteins. 相似文献
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104.
Christine A. Bricault James M. Kovacs Joseph P. Nkolola Karina Yusim Elena E. Giorgi Jennifer L. Shields James Perry Christy L. Lavine Ann Cheung Katharine Ellingson-Strouss Cecelia Rademeyer Glenda E. Gray Carolyn Williamson Leonidas Stamatatos Michael S. Seaman Bette T. Korber Bing Chen Dan H. Barouch 《Journal of virology》2015,89(5):2507-2519
105.
Ecological index of maturity to evaluate the vegetation disturbance of areas affected by restoration work: a practical example of its application in an area of the Southern Alps
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This paper presents the ecological index of maturity (EIM), a new index for evaluating the vegetation disturbance of an area affected by environmental restoration measures based on the study of plant communities. The EIM is the result of the unification and development of some floristic‐vegetational indices devised by Taffetani and Rismondo (2009) and Rismondo et al. (2011) to assess the ecological functionality of agro‐ecosystems. The EIM provides a value between 0 (high vegetation disturbance) and 9 (undisturbed vegetation), obtained by considering three distinct variables of the species of a plant community: phytosociological class, chorotype, and coverage. Starting from the measure of the degree of maturity of the vegetation (dynamic stage of succession), EIM values tend to decrease the greater the coverage of exotic species and the lower the coverage of endemic species present in the plant community. A practical example, in which the EIM is applied to a mountainous area in the Southern Alps (Northern Italy) that, following a landslide, was subject to soil bioengineering work aimed at slope stabilization and environmental restoration, is provided in order to understand the importance and features of the EIM. 相似文献
106.
Cherkasov A Hsing M Zoraghi R Foster LJ See RH Stoynov N Jiang J Kaur S Lian T Jackson L Gong H Swayze R Amandoron E Hormozdiari F Dao P Sahinalp C Santos-Filho O Axerio-Cilies P Byler K McMaster WR Brunham RC Finlay BB Reiner NE 《Journal of proteome research》2011,10(3):1139-1150
Mortality attributable to infection with methicillin-resistant Staphylococcus aureus (MRSA) has now overtaken the death rate for AIDS in the United States, and advances in research are urgently needed to address this challenge. We report the results of the systematic identification of protein-protein interactions for the hospital-acquired strain MRSA-252. Using a high-throughput pull-down strategy combined with quantitative proteomics to distinguish specific from nonspecific interactors, we identified 13,219 interactions involving 608 MRSA proteins. Consecutive analyses revealed that this protein interaction network (PIN) exhibits scale-free organization with the characteristic presence of highly connected hub proteins. When clinical and experimental antimicrobial targets were queried in the network, they were generally found to occupy peripheral positions in the PIN with relatively few interacting partners. In contrast, the hub proteins identified in this MRSA PIN that are essential for network integrity and stability have largely been overlooked as drug targets. Thus, this empirical MRSA-252 PIN provides a rich source for identifying critical proteins essential for network stability, many of which can be considered as prospective antimicrobial drug targets. 相似文献
107.
Background
Therapy for neural lesions or degenerative diseases relies mainly on finding transplantable active precursor cells. Identifying them in peripheral tissues accessible for biopsy, outside the central nervous system, would circumvent the serious immunological and ethical concerns impeding cell therapy.Methodology/Principal Findings
In this study, we isolated neural progenitor cells in cultured adult skeletal muscle from transgenic mice in which nestin regulatory elements control GFP expression. These cells also expressed the early neural marker Tuj1 and light and heavy neurofilament but not S100β, indicating that they express typical neural but not Schwann cell markers. GFP+/Tuj1+ cells were also negative for the endothelial and pericyte markers CD31 and α-smooth muscle actin, respectively. We established their a) functional response to glutamate in patch-clamp recordings; b) interstitial mesenchymal origin; c) replicative capacity; and d) the environment necessary for their survival after fluorescence-activated cell sorting.Conclusions/Significance
We propose that the decline in nestin-GFP expression in muscle progenitor cells and its persistence in neural precursor cells in muscle cultures provide an invaluable tool for isolating a population of predifferentiated neural cells with therapeutic potential. 相似文献108.
Ré VE Culasso AC Mengarelli S Farías AA Fay F Pisano MB Elbarcha O Contigiani MS Campos RH 《PloS one》2011,6(5):e19471
The Hepatitis C Virus Genotype 2 subtype 2c (HCV-2c) is detected as a low prevalence subtype in many countries, except in Southern Europe and Western Africa. The current epidemiology of HCV in Argentina, a low-prevalence country, shows the expected low prevalence for this subtype. However, this subtype is the most prevalent in the central province of Córdoba. Cruz del Eje (CdE), a small rural city of this province, shows a prevalence for HCV infections of 5%, being 90% of the samples classified as HCV-2c. In other locations of Córdoba Province (OLC) with lower prevalence for HCV, HCV-2c was recorded in about 50% of the samples. The phylogenetic analysis of samples from Córdoba Province consistently conformed a monophyletic group with HCV-2c sequences from all the countries where HCV-2c has been sequenced. The phylogeographic analysis showed an overall association between geographical traits and phylogeny, being these associations significant (α = 0.05) for Italy, France, Argentina (places other than Córdoba), Martinique, CdE and OLC. The coalescence analysis for samples from CdE, OLC and France yielded a Time for the Most Common Recent Ancestor of about 140 years, whereas its demographic reconstruction showed a “lag” phase in the viral population until 1880 and then an exponential growth until 1940. These results were also obtained when each geographical area was analyzed separately, suggesting that HCV-2c came into Córdoba province during the migration process, mainly from Europe, which is compatible with the history of Argentina of the early 20th century. This also suggests that the spread of HCV-2c occurred in Europe and South America almost simultaneously, possibly as a result of the advances in medicine technology of the first half of the 20th century. 相似文献
109.
110.
Beer C Horner B Flicker L Scherer S Lautenschlager NT Bretland N Flett P Schaper F Almeida OP 《PloS one》2011,6(11):e28155