全文获取类型
收费全文 | 250篇 |
免费 | 14篇 |
国内免费 | 9篇 |
出版年
2015年 | 14篇 |
2014年 | 9篇 |
2013年 | 8篇 |
2012年 | 6篇 |
2011年 | 13篇 |
2010年 | 8篇 |
2009年 | 7篇 |
2008年 | 3篇 |
2007年 | 6篇 |
2006年 | 8篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2002年 | 3篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1994年 | 6篇 |
1985年 | 3篇 |
1982年 | 3篇 |
1977年 | 2篇 |
1954年 | 3篇 |
1947年 | 3篇 |
1946年 | 2篇 |
1945年 | 2篇 |
1944年 | 3篇 |
1943年 | 3篇 |
1941年 | 2篇 |
1940年 | 7篇 |
1939年 | 3篇 |
1938年 | 9篇 |
1936年 | 3篇 |
1935年 | 7篇 |
1934年 | 6篇 |
1933年 | 5篇 |
1932年 | 2篇 |
1931年 | 3篇 |
1930年 | 4篇 |
1929年 | 5篇 |
1928年 | 5篇 |
1927年 | 2篇 |
1925年 | 4篇 |
1922年 | 2篇 |
1921年 | 3篇 |
1920年 | 2篇 |
1919年 | 6篇 |
1918年 | 6篇 |
排序方式: 共有273条查询结果,搜索用时 31 毫秒
41.
铁皮石斛的离体开花 总被引:9,自引:0,他引:9
铁皮石斛(Dendrobium candidum),为一种野生兰科植物,在栽培条件下,从种子萌发到开花通常需要3~4a.研究了多种植物激素和多胺对该种石斛组织培养中花芽形成的影响,结果表明在培养基中加入合适浓度的亚精胺(spermidine)或BA(6-苄基腺嘌呤),或同时加入NAA(萘乙酸)和BA均可诱导原球茎或由之形成的无根小苗在3~6个月开花,频率在31.6%~45.8%.当将原球茎在加有ABA(脱落酸)的培养基上预培养后再移到加有BA的培养基上,花芽形成的频率可提高到平均达82.8%(个别实验中可达100%),这种诱导提早开花的现象也与实验材料的发育阶段(原球茎、无根小苗、已生根的小苗)有关,通常发生在根的形成受到完全或部分抑制的情况中. 相似文献
42.
43.
Efficiencies of different genes and different tree-building methods in recovering a known vertebrate phylogeny 总被引:24,自引:6,他引:18
The relative efficiencies of different protein-coding genes of the
mitochondrial genome and different tree-building methods in recovering a
known vertebrate phylogeny (two whale species, cow, rat, mouse, opossum,
chicken, frog, and three bony fish species) was evaluated. The
tree-building methods examined were the neighbor joining (NJ), minimum
evolution (ME), maximum parsimony (MP), and maximum likelihood (ML), and
both nucleotide sequences and deduced amino acid sequences were analyzed.
Generally speaking, amino acid sequences were better than nucleotide
sequences in obtaining the true tree (topology) or trees close to the true
tree. However, when only first and second codon positions data were used,
nucleotide sequences produced reasonably good trees. Among the 13 genes
examined, Nd5 produced the true tree in all tree-building methods or
algorithms for both amino acid and nucleotide sequence data. Genes Cytb and
Nd4 also produced the correct tree in most tree-building algorithms when
amino acid sequence data were used. By contrast, Co2, Nd1, and Nd41 showed
a poor performance. In general, large genes produced better results, and
when the entire set of genes was used, all tree-building methods generated
the true tree. In each tree-building method, several distance measures or
algorithms were used, but all these distance measures or algorithms
produced essentially the same results. The ME method, in which many
different topologies are examined, was no better than the NJ method, which
generates a single final tree. Similarly, an ML method, in which many
topologies are examined, was no better than the ML star decomposition
algorithm that generates a single final tree. In ML the best substitution
model chosen by using the Akaike information criterion produced no better
results than simpler substitution models. These results question the
utility of the currently used optimization principles in phylogenetic
construction. Relatively simple methods such as the NJ and ML star
decomposition algorithms seem to produce as good results as those obtained
by more sophisticated methods. The efficiencies of the NJ, ME, MP, and ML
methods in obtaining the correct tree were nearly the same when amino acid
sequence data were used. The most important factor in constructing reliable
phylogenetic trees seems to be the number of amino acids or nucleotides
used.
相似文献
44.
Arthur M. Edelman Wei-Hsung Lin Donna J. Osterhout Mark K. Bennett Mary B. Kennedy Edwin G. Krebs 《Molecular and cellular biochemistry》1990,97(1):87-98
Brain type II Ca2+/calmodulin-dependent protein kinase was found to phoshorylate smooth muscle myosin, incorporating maximally 2 mol of phosphoryl per mol of myosin, exclusively on the 20,000 dalton light chain subunit. After maximal phosphorylation of myosin or the isolated 20,000 dalton light chain subunit by myosin light chain kinase, the addition of type II Ca2+/calmodulin-dependent protein kinase led to no further incorporation indicating the two kinases phosphorylated a common site. This conclusion was supported by two dimensional mapping of tryptic digests of myosin phosphorylated by the two kinases. By phosphoamino acid analysis the phosphorylated residue was identified as a serine. The phosphorylation by type II Ca 2+/calmodulin-dependent protein kinase of myosin resulted in enhancement of its actin-activated Mg2+-ATPase activity. Taken together, these data strongly support the conclusion that type II Ca2+/calmodulin-dependent protein kinase phosphorylates the same amino acid residue on the 20,000 dalton light chain subunit of smooth muscle myosin as is phosphorylated by myosin light chain kinase and suggest an alternative mechanism for the regulation of actin-myosin interaction.Abbreviations SDS-PAGE
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
- EGTA
Ethylene Glycol Bis (-amino-ethyl ether)-N,N,N,N-Tetraacetic Acid
- DTT
Dithiothreitol
- LC20
Gizzard Smooth Muscle Phosphorylatable 20 kDa Myosin Light Chain
- LC17
Gizzard Smooth Muscle, 17 kDa Myosin Light Chain
- H Chain
Gizzard Smooth Muscle 200 kDa Myosin Heavy Chain
- TPCK
L-1-Tosylamido-2-Phenylethyl Chloromethyl Ketone
- MOPS
3-(N-morpholino) Propanesulfonic Acid 相似文献
45.
W. J. V. Osterhout 《The Journal of general physiology》1921,4(1):1-9
An electrical current passing through a living plant flows partly through the cell wall and partly through the protoplasm. The relative amounts of these two portions of the current can be calculated. The outcome of such calculations shows that the conclusions drawn from the study of the resistance of the tissue as a whole apply also to the resistance of the protoplasm, and consequently to the permeability of the protoplasm to ions. 相似文献
46.
Using multinucleate cells of Nitella 2 or 3 inches in length it is possible to kill one end with chloroform without producing at the other any immediate alteration which can be detected by our present methods. When a spot in external contact with sap is killed its potential difference falls approximately to zero and it is therefore possible to measure the potential difference across the protoplasm at any desired point merely by leading off from that point to the one where the protoplasm has been killed. The results indicate that the inner and outer protoplasmic surfaces differ, for when both surfaces are in contact with the same solution (cell sap) there is an electromotive force of about 15.9 millivolts, the inner surface being positive to the outer (i.e. the positive current tends to flow from the inner surface through the electrometer to the outer surface). The situation resembles that in Valonia where the corresponding value (with Valonia sap applied to the outside) has been reported as about 14.5 millivolt (the inner surface being positive to the outer). It would seem appropriate to designate this as radial polarity. 相似文献
47.
Experiments on cutting confirm the prediction that the current of injury will be positive when the cell is in contact with concentrated solutions and negative with dilute solutions. They support the idea that the protoplasm is made up of layers differing considerably in their properties, each having a death curve of simple and regular form, the more rapid alteration of the outer layer making the protoplasm more positive and the more rapid alteration of the inner making it more negative. From the point where the cell is cut a wave of some sort, which we may for convenience call a death wave, passes along the cell, setting up at each point it touches a death process which has the greater speed and intensity the nearer it is to the cut. 相似文献
48.
W. J. V. Osterhout 《The Journal of general physiology》1944,28(1):17-22
The vacuolar surface of Nitella is covered with a non-aqueous film too thin to be visible as a separate membrane. The motion of the protoplasm may subject this film to a good deal of mechanical disturbance. Apparently this does not rupture the film for no dye escapes into the protoplasm as the result of such disturbance when the vacuolar sap is deeply stained with neutral red or brilliant cresyl blue. When the deeply stained central vacuole breaks up into several smaller vacuoles, leaving the outer protoplasmic surface in its normal position, there is no evidence of the escape of dye into the protoplasm through the film surrounding the vacuole. 相似文献
49.
Thrombospondin (TSP) is a prominent constituent of the extracellular matrix of the developing nervous system. We have examined the effects of TSP on the morphological differentiation of neurons. In short-term cultures (less than or equal to 24 hr) of embryonic rat sympathetic neurons, TSP stimulated neurite outgrowth, causing significant increase in the number of processes and their length. Similar effects were observed in cultures of rat dorsal root ganglion, hippocampal, and cerebral cortical neurons. Moreover, in cultures of central neurons, TSP was more effective than laminin in enhancing process extension. Analysis of long-term (5-7 days) cultures of sympathetic neurons indicated that processes formed in the presence of TSP had the cytochemical characteristics of axons. Thus, TSP can influence neuronal development by selectively enhancing axonal growth. The neurite-promoting region of the molecule was identified using a panel of monoclonal antibodies targeted to different regions of the protein. Process outgrowth could be totally inhibited with antibody A4.1, which recognizes the stalk region of TSP. These data suggest that the neurite-promoting activity is localized to a single region of the TSP molecule. 相似文献
50.
When 0.005 M NH4Cl is added to sea water containing cells of Valonia macrophysa ammonia soon appears in the sap and may reach a concentration inside over 40 times as great as outside. It appears to enter as undissociated NH3 (or NH4OH) and tends to reach a pseudoequilibrium in which the activity of undissociated NH3 (or NH4OH) is the same inside and outside. When ammonia first enters, the pH value of the sap rapidly rises but it soon reaches a maximum and subsequently falls off. At the same time there is an increase of halide in the sap which, however, does not run a parallel course to the ammonia accumulation, but it comes to a new equilibrium value and remains constant. The increase in NH3 in the sap is accompanied by a decrease in the concentration of K. As NH3 enters the specific gravity of the sap decreases and the cells rise to the surface and continue to grow as floating organisms. The growth of the cells is increased. 相似文献