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61.
Structures of the iron-sulfur flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus
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Andrade SL Cruz F Drennan CL Ramakrishnan V Rees DC Ferry JG Einsle O 《Journal of bacteriology》2005,187(11):3848-3854
Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster. In order to gain further insight into the function and properties of ISF, the three-dimensional structures of two ISF homologs, one from the thermophilic methanogen Methanosarcina thermophila and one from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus, were determined. The structures indicate that ISF assembles to form a tetramer and that electron transfer between the two types of redox cofactors requires oligomerization to juxtapose the flavin mononucleotide and [4Fe:4S] cluster bound to different subunits. This is only possible between different monomers upon oligomerization. Fundamental differences in the surface properties of the two ISF homologs underscore the diversity encountered within this protein family. 相似文献
62.
63.
CD4 T cell-dependent autoimmunity against a melanocyte neoantigen induces spontaneous vitiligo and depends upon Fas-Fas ligand interactions 总被引:5,自引:0,他引:5
Lambe T Leung JC Bouriez-Jones T Silver K Makinen K Crockford TL Ferry H Forrester JV Cornall RJ 《Journal of immunology (Baltimore, Md. : 1950)》2006,177(5):3055-3062
Better understanding of tolerance and autoimmunity toward melanocyte-specific Ags is needed to develop effective treatment for vitiligo and malignant melanoma; yet, a systematic assessment of these mechanisms has been hampered by the difficulty in tracking autoreactive T cells. To address this issue, we have generated transgenic mice that express hen egg lysozyme as a melanocyte-specific neoantigen. By crossing these animals to a hen egg lysozyme-specific CD4 TCR transgenic line we have been able to track autoreactive CD4+ T cells from their development in the thymus to their involvement in spontaneous autoimmune disease with striking similarity to human vitiligo vulgaris and Vogt-Koyanagi-Harada syndrome. Our findings show that CD4-dependent destruction of melanocytes is partially inhibited by blocking Fas-Fas ligand interactions and also highlights the importance of local control of autoimmunity, as vitiligo remains patchy and never proceeds to confluence even when Ag and autoreactive CD4+ T cells are abundant. Immune therapy to enhance or suppress melanocyte-specific T cells can be directed at a series of semiredundant pathways involving tolerance and cell death. 相似文献
64.
van Hall T Wolpert EZ van Veelen P Laban S van der Veer M Roseboom M Bres S Grufman P de Ru A Meiring H de Jong A Franken K Teixeira A Valentijn R Drijfhout JW Koning F Camps M Ossendorp F Kärre K Ljunggren HG Melief CJ Offringa R 《Nature medicine》2006,12(4):417-424
Defects in major histocompatibility complex (MHC) class I-restricted antigen presentation are frequently observed in human cancers and result in escape of tumors from cytotoxic T lymphocyte (CTL) immune surveillance in mice. Here, we show the existence of a unique category of CTLs that can prevent this escape. The CTLs target an alternative repertoire of peptide epitopes that emerge in MHC class I at the surface of cells with impaired function of transporter associated with antigen processing (TAP), tapasin or the proteasome. These peptides, although derived from self antigens such as the commonly expressed Lass5 protein (also known as Trh4), are not presented by normal cells. This explains why they act as immunogenic neoantigens. The newly discovered epitopes can be exploited for immune intervention against processing-deficient tumors through adoptive T-cell transfer or peptide vaccination. 相似文献
65.
First DNA malaria vaccine on trial in Africa 总被引:3,自引:0,他引:3
Ferry G 《Current biology : CB》2000,10(22):R810-R811
66.
Wiggenhauser PS Müller DF Melchels FP Egaña JT Storck K Mayer H Leuthner P Skodacek D Hopfner U Machens HG Staudenmaier R Schantz JT 《Cell and tissue research》2012,347(3):747-757
Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration. Sponge-like polyurethane scaffolds were prepared by mold casting with methylal as foaming agent, whereas polycaprolactone scaffolds with highly regular stacked-fiber architecture were fabricated with fused deposition modeling. Both scaffold types were seeded with human adipose tissue-derived precursor cells, cultured and implanted in nude mice using a femoral arteriovenous flow-through vessel loop for angiogenesis. In vitro, cells attached to both scaffolds and differentiated into adipocytes. In vivo, angiogenesis and adipose tissue formation were observed throughout both constructs after 2 and 4?weeks, with angiogenesis being comparable in seeded and unseeded constructs. Fibrous tissue formation and adipogenesis were more pronounced on polyurethane foam scaffolds than on polycaprolactone prototyped scaffolds. In conclusion, both scaffold designs can be effectively used for adipose tissue engineering. 相似文献
67.
68.
Faure C Raynaud-Simon A Ferry A Daugé V Cynober L Aussel C Moinard C 《Amino acids》2012,42(4):1425-1433
Protein energy malnutrition in the elderly causes preferential loss of muscle mass which is associated with poor functional
states. Leucine and citrulline are able to stimulate muscle protein synthesis in aged rats but no study has been undertaken
to evaluate their effect on muscle function. Sprague–Dawley male rats aged 23 months were used in the experiment. Part of
them were subjected to a dietary restriction for 12 weeks and then assigned to four groups: a group was euthanized (restricted
group), and the others were refed for 1 week with either a leucine-, a citrulline-supplemented diet, or a standard diet. The
other rats were fed ad libitum. Muscle mass and motor activity significantly increased during the refeeding with either leucine
or citrulline (respectively, +51 and +37% for muscle mass, P < 0.05). The improvement of muscle mass and of motor activity induced by leucine and citrulline was highly associated with
that of maximal tetanic isometric force (r = 0.769, P < 0.0001; r = 0.389, P < 0.05, respectively) but only leucine improved maximal tetanic isometric force (+101%, P < 0.05). In conclusion, this is the first study to demonstrate the ability of two amino acids (leucine and citrulline) to
modulate muscle function. 相似文献
69.
Fould B Lamamy V Guenin SP Ouvry C Cogé F Boutin JA Ferry G 《Protein science : a publication of the Protein Society》2012,21(9):1323-1333
The human thioredoxin (TRX)-interacting protein is found in multiple subcellular compartments and plays a major role in redox homeostasis, particularly in the context of metabolism (e.g., lipidemia and glycemia) and apoptosis. A molecular approach to the protein's modus operandi is still needed because some aspects of the TRX-interacting protein-mediated regulation of TRX are not clearly understood. To this end, His-tagged TRX-interacting proteins were over-expressed in Escherichia coli. Because the protein is expressed mainly in inclusion bodies, it was denatured in high concentrations of guanidium hydrochloride, centrifuged, and purified by Ni-NTA affinity chromatography. His-TRX-interacting protein was then refolded by dialysis and its restructuring monitored by circular dichroism spectrometry. This preparation resulted in the formation of a covalent complex with recombinant human TRX, demonstrating that association occurs without the intervention of other partner proteins. Multiple cysteine-to-serine mutants of TRX-interacting protein were produced and purified. These mutations were efficient in limiting the formation of disulfide-linked homo-oligomers in an oxidizing environment. The mutants were also used to gain functional insight into the formation of the TRX-interacting protein-TRX complexes. These complexes were able to form in the absence of internal disulfide bridges. A mutant with all but one cysteine changed to serine (Cys(247) ) also showed an enhanced capacity to form complexes with TRX demonstrating, in a pure molecular system, that this particular cysteine is likely responsible for the disulfide bridge between TRX-interacting protein and TRX. 相似文献
70.
Pan W Khayhan K Hagen F Wahyuningsih R Chakrabarti A Chowdhary A Ikeda R Taj-Aldeen SJ Khan Z Imran D Sjam R Sriburee P Liao W Chaicumpar K Ingviya N Mouton JW Curfs-Breuker I Boekhout T Meis JF Klaassen CH 《PloS one》2012,7(3):e32868