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141.
142.
When isolated intact chloroplasts or cells from spinach (Spinacia oleracea L.) leaves are incubated in the light in the absence of CO2, their capacity for subsequent CO2-dependent photosynthetic oxygen evolution is drastically decreased. This inhibition is light and oxygen-dependent and can be prevented by addition of bicarbonate. It is concluded that the normal dissipation of photosynthetic energy by carbon assimilation and in processes related to photorespiration is an essential condition for the physiological stability of illuminated intact chloroplasts and cells.Abbreviation chl
chlorophyll 相似文献
143.
Lymphocyte subtypes in mouse bone marrow have been analyzed according to the combination of three surface membrane markers, IgM molecules, Fc, and complement receptors (FcR, CR), expressed simultaneously on individual cells. Marrow cell suspensions were depleted of IgM-, FcR-, and CR-bearing cells, respectively, by differential centrifugation after rosetting with appropriately sensitized erythrocytes. After rerosetting, the FcR-depleted marrow fraction showed many IgM + ve but no CR + ve small lymphocytes, the CR-depleted fraction contained both IgM + ve and FcR + ve small lymphocytes, while the IgM-depleted fraction showed many FcR + ve but few CR + ve small lymphocytes. Radioautography after [3H]thymidine labeling for 1 and 4 days in vivo demonstrated an active turnover of the various lymphocyte subtypes, particularly rapid for (IgM ? ve, FcR + ve) cells. The results demonstrate the presence of three subtypes of marrow small lymphocytes which correspond with three proposed stages in the maturation of newly formed primary B lymphocytes; (a) null cells (IgM ? ve, FcR ? ve, CR ? ve), (b) IgM + ve, FcR ? ve, CR ? ve, and (c) IgM + ve, FcR + ve, CR + ve. In addition, the turnover of a sizeable population of null small lymphocytes which bear FcR, without IgM and CR, suggests the genesis of a distinct marrow lymphocyte lineage, not previously described. 相似文献
144.
Papageorgiou PC Pourdjabbar A Amfilochiadis AA Diamandis EP Boomsma F Osmond DH 《American journal of physiology. Heart and circulatory physiology》2004,286(3):H837-H846
"New pressor protein" (NPP) derived from normal human plasma is an extra renal enzyme that shares strong sequence homology with human coagulation beta-FXIIa. Under our bioassay conditions, human NPP (10-20 microl plasma equivalent/ approximately 300 g rat iv) can raise the systolic blood pressure (SBP) by 40-50 mmHg, the diastolic blood pressure (DBP) by 15-20 mmHg, and the heart rate (HR) by 70-90 beats/min. Plasma epinephrine (of adrenal medullary origin) and norepinephrine rise by about 50- and 10-fold, respectively. Because beta-FXIIa is not normally associated with pressor properties, we endeavored to substantiate that the hypertensive effects of impure NPP preparations used in our experiments are attributable to their content of beta-FXIIa. We carried out comparisons with highly purified (>90%) commercial human beta-FXIIa and found that by gel filtration (Sephadex G-100 and G-75), NPP bioactivity appeared in the approximately 30-kDa elution zone, consistent with the molecular mass of beta-FXIIa. Retention time using fast-protein liquid chromatography anion exchange chromatography was identical. Molecular mass and comigration were confirmed by SDS-PAGE gel electrophoresis, and the recovered approximately 30-kDa protein bands yielded beta-FXIIa fragments identified by mass spectrometry. Matched doses of the NPP preparations produced dose-response curves very similar to those elicited by beta-FXIIa with respect to increments of SBP, DBP, and HR, whereas plasma catecholamine increments were generally comparable. We propose that beta-FXIIa is substantially, if not exclusively, responsible for the observed effects of our NPP preparations and that this points to a novel axis connecting the FXII coagulation cascade and the sympathoadrenal gland to other cardiovascular regulatory mechanisms. 相似文献
145.
Influence of nitrogen supply and growth irradiance on photoinhibition and recovery in Heuchera americana (Saxifragaceae) 总被引:1,自引:0,他引:1
Prior work demonstrated that Heuchera americana, an evergreen herb inhabiting the deciduous forest understory in the southeastern United States, has a 3-4-fold greater photosynthetic capacity under the low-temperature, strong-light, open canopies of winter compared to the high-temperature, weak-light, closed canopies of summer. Moreover, despite the reductions in soil nitrogen, the chilling temperatures, and the increased quantum flux associated with winter, chronic photoinhibition was not observed in this species at this time of the year. We were interested in the photosynthetic acclimation and photoinhibition characteristics of this species when grown under contrasting light and nitrogen regimes. Newly expanded shade-acclimated leaves of forest-grown plants exposed to strong light varying in intensity and duration at 25°C showed a reduction in Fv/Fm (the ratio of variable to maximum room temperature chlorophyll fluorescence measured after dark adaptation), which was correlated with a decline in øa (the intrinsic quantum yield of CO2-saturated O2 evolution on an absorbed light basis). Plants grown in the glasshouse under contrasting light (high and low light; HL and LL, respectively) and nitrogen supply (high and low nitrogen; HN and LN, respectively) regimes showed that photosynthetic acclimation to HL was impaired in LN regimes. The HL-LN plants also had the lowest values of Fv/Fm and of ø on both incident and absorbed light bases and had 50% less chlorophyll (per unit area) compared to plants from other growth regimes. Controlled exposure to bright light at low temperatures (2-3°C) for 3 h resulted in a sharp decrease in Fv/Fm (and rise in Fo, the minimum fluorescence yield) in all plants. Shade-grown plants from both N regimes were highly susceptible to chronic photoinhibition, as indicated by a greater reduction in Fv/Fm and incomplete recovery after 18 h in weak light at 25°C. The HL-HN plants were the least susceptible to chronic photoinhibition, having the smallest decrease in Fv/Fm with near full recovery within 6 h. The decline in Fv/Fm in HL-LN plants was comparable to that of shade-acclimated plants, but recovered fully within 6 h. Low-N plants from both light regimes displayed greater increases in Fo which did not return to pretreatment levels after 18 h of recovery. These studies indicate that HL-LN plants were sensitive to chronic photoinhibition and, at the same time, had a high capacity for dynamic photoinhibition. Experimental garden studies showed that H. americana grown in an open field in summer were photoinhibited and did not fully recover overnight or during extended periods of weak light. These results are discussed in relation to the photosynthetic acclimation of H. americana under natural conditions. 相似文献
146.
Diagnosis of the Earliest Strain-Specific Interactions between Tobacco Mosaic Virus and Chloroplasts of Tobacco Leaves in Vivo by Means of Chlorophyll Fluorescence Imaging 总被引:9,自引:3,他引:6
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Fluorescence imaging was used to diagnose early stages of the strain-specific interactions between tobacco mosaic virus (strain PV230) and chloroplasts following infection of tobacco leaves (Nicotiana tabacum cv Xanthi). The earliest indication of interaction in tissues that ultimately become chlorotic was a reduction in chlorophyll fluorescence, and there was little fluorescence quenching compared with adjacent healthy tissues. Subsequently, fluorescence increased but remained unquenched. In the late stages fluorescence declined again in chlorotic regions as the chloroticmosaic symptoms developed. These in vivo data showing altered fluorescence yields confirm strain-specific interaction of virus coat protein with photosystem II (PSII) components in vitro, leading to photoinhibition and photooxidation of chlorophyll in infected cells and the development of visible chlorotic-mosaic symptoms. Although mechanisms leading to the low, unquenched fluorescence condition are not known, the intermediate high, unquenched fluorescence condition is consistent with impaired PSII electron transport as measured in vitro. Fluorescence lesions appear more rapidly and develop more extensively in high light, consistent with the faster and larger extent of symptom formation in high-light-grown leaves than in low-light-grown leaves. 相似文献
147.
Summary Diurnal measurements of low temperature (77K) fluorescence at 690 nm (PS II) from north, south, east, and west facing cladode surfaces of Opuntia basilaris in Death Valley, California were made on six occasions during 1985. The absolute levels of F
o(instantaneous fluorescence) and F
m(maximum fluorescence), as well as the ratio F
v/F
m(variable fluorescence, F
m-F
o, over maximum fluorescence), were greater in the north face relative to the other faces. Diurnal decreases in F
o, F
mand F
v/F
mwere found concomitant with increases in incident photon flux area density (PFD). F
v/F
mwas fairly low throughout the year, indicative of photoinhibition, but became somewhat elevated after a spring rain. In early fall the quantum yield of the south face was considerably depressed relative to that of the north face, and corresponding differences were observed in F
v/F
m. A decrease in PFD during growth of glasshouse plants led to an increase in chlorophyll concentration, F
oand F
m, but not F
v/F
m. Although there was some variability in the quantum yield of well watered glasshouse cladodes, a correlation was found between quantum yield and the light and CO2 saturated rate of photosynthesis. When O. basilaris was water stressed under glasshouse conditions, reductions in quantum yield, F
m, and F
v/F
mwere observed. Reductions in F
v/F
malways indicated a reduced quantum yield, although the converse was not necessarily so in well watered glasshouse plants. The results of this study indicate that O. basilaris is likely to experience photoinhibition throughout much of its life in Death Valley.Abbreviations
CAM
crassulacean acid metabolism
-
MPa
megapascal
-
PFD
photon flux area density
-
PS II
photosystem II
-
vater potential
-
F
o
instantaneous fluorescence
-
F
m
maximum fluoescence
-
F
o
variable fluorescence 相似文献
148.
Summary Crassulacean acid metabolism (CAM) was studied in a tropical epiphytic fern, Pyrrosia longifolia, from a fully sun-exposed and from a very shaded site in Northern Queensland, Australia. Measurements of instantaneous net CO2 exchange showed carbon gain via CO2 dark fixation with some net CO2 uptake also occuring during late afternoon, in both sun and shade fronds. Maximum rates of net CO2 uptake and the nocturnal increase in titratable acidity were lower in shade than in sun fronds. 13C values of sun and shade fronds were not significantly different, and ranged between-14 and-15 suggesting that, in the long term, carbon gain was mainly via CO2 dark fixation. Sun fronds had a higher light compensation point of photosynthesis than shade fronds but the same quantum yield. Yet there was no acclimation of photosynthetic O2 evolution, (measured at 5% CO2) in sun and shade fronds and photosynthesis saturated at between 200 and 400 mol quanta m-2 s-1. Use of higher light intensities for photosynthesis of sun fronds was probably precluded by low nutrient availability. Total nitrogen was less than 1% of dry weight in fully expanded sun and shade fronds. Exposure of shade fronds to full sunlight for 6 h led to a 60% decline in the quantum yield of photosynthesis and to a decline in variable fluorescence measured at room temperature. Photoinhibition by high light was also observed in Hoya nicholsoniae, a rainforest climber growing in deep shade. This species also exhibited CAM as demonstrated by nocturnal net CO2 uptake, nocturnal acidification and a 13C value of-14. Photosynthetic O2 evolution in this species was saturated at 2.5% of full sunlight. Two species of Dendrobium (Orchidaceae) from sun-exposed sites, one species exhibiting CAM and the other one exhibiting net CO2 uptake exclusively during daytime via conventional C3 photosynthesis, showed similar light response curves and the same quantum yield for photosynthetic O2 evolution. 相似文献
149.
E A Wilczynski D H Osmond 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1986,182(2):208-214
Beta blockade with propranolol for 7 days in healthy normotensive dogs produced a sustained 20-25% drop in heart rate, but only a transient suppression of blood pressure. Plasma renin activity and prorenin were also suppressed transiently, suggesting that both are under beta-receptor regulation. Bilateral nephrectomy (2NX) was followed by rapid clearance of renin from the circulation, at a rate that was minimally influenced by beta blockade. In contrast, the plasma prorenin level rose markedly to a peak within an hour after surgery, leveled off during the next 24 hr, dropped almost toward the pre-2NX baseline by 48 hr, but proceeded to rise again between 48 and 120 hr. Propranolol administration before and during the 2NX period reduced the detectable prorenin, suggesting that its extrarenal source is under beta-adrenergic regulation. The rapid increment of prorenin after 2NX suggests that extrarenal prorenin may have constituted part of the total plasma prorenin before 2NX, and/or had developed sufficiently quickly afterwards to replace and exceed the disappearing renal prorenin. Any fresh increment beyond 48 hr could presumably have been only extrarenal. These observations suggest the existence of a rich beta-regulated extrarenal source of prorenin capable of rapidly supplying the plasma. However, no renin-angiotesin was apparently produced from this prorenin in the nephrectomized state, implying the lack of renal "convertase," without which the prorenin convertase mechanism as a whole was rendered ineffective. The source of the extrarenal prorenin and the identity of the renal convertase remain to be established. 相似文献
150.
Malate Metabolism in the Dark After CO(2) Fixation in the Crassulacean Plant Kalanchoë tubiflora
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The metabolism of [13C]malate was studied in the Crassulacean plant Kalanchoë tubiflora following exposure to 13CO2 for 2 hour intervals during a 16 hour dark cycle. Nuclear magnetic resonance spectroscopy of [13C]malate extracted from labeled tissue revealed that the transient flux of malate to the mitochondria, estimated by the randomization of [4-13C]malate to [1- 13C]malate by fumarase, varied substantially during the dark period. At both 15 and 25°C, the extent of malate label randomization in the mitochondria was greatest during the early and late parts of the dark period and was least during the middle of the night, when the rate of 13CO2 uptake was highest. Randomization of labeled malate continued for many hours after malate synthesis had initially occurred. Internally respired 12CO2 also served as a source of carbon for malate formation. At 15°C, 15% of the total malate was formed from respired 12CO2, while at 25°C, 49% of the accumulated malate was derived from respired 12CO2. Some of the malate synthesized from external 13CO2 was also respired during the night. The proportion of the total [13C]malate respired during the dark period was similar at 15 and 25°C, and respiration of newly formed [13C]malate increased as the night period progressed. These data are discussed with regard to the relative fluxes of malate to the mitochondria and the vacuole during dark CO2 fixation. 相似文献