浙江省SARS冠状病毒分离与系统进化树分析

从浙江省3例SARS患者中收集含漱液标本,经处理后接种Vero、RD、VeroE6和Hep-2细胞进行病毒分离,培养3d后在Vero和RD细胞中可观察到细胞病变。从细胞培养上清中提取病毒核酸,用SARS冠状病毒特异性引物进行RT-PCR,并经测序证实从3份临床样本中分离到2株SARS冠状病毒株。对其中1株病毒的基因组进行了全序列测定并作系统进化树分析显示浙汀省SARS冠状病毒株与新加坡2774株和台湾TW1株最为接近。     

乳腺纤维瘤蛋白质组的凝胶内差异显示电泳分析

目的:建立具有高分辨率和稳定性的乳腺纤维瘤组织蛋白质组的双向电泳图谱,并对其进行差异蛋白质组分析.方法:取乳腺纤维瘤病患者病变部位及正常乳腺组织,匀浆提取乳腺组织总蛋白,分别用Cy3或Cy5标记,每一时Cy3和cy5标记样品都与一个Cy2标记的内标等量混合,上样于同一胶中进行电泳分离,经不同光激发下扫描得到不同样品的蛋白质组图谱.所获得的图谱经DeCyder软件分析.结果:在乳腺纤维瘤病的病变组织中,有37个蛋白质表达水平显著增加,另外8个蛋白质表达水平显著下降.结论:分析所得的45个差异蛋白质可能与乳腺纤维瘤疾病的发生与发展有关.     

Chemical and optical changes in freshwater dissolved organic matter exposed to solar radiation

We studied the chemical and optical changes inthe dissolved organic matter (DOM) from twofreshwater lakes and a Sphagnum bog afterexposure to solar radiation. Stable carbonisotopes and solid-state ¹³C-NMR spectraof DOM were used together with optical andchemical data to interpret results fromexperimental exposures of DOM to sunlight andfrom seasonal observations of two lakes innortheastern Pennsylvania. Solar photochemicaloxidation of humic-rich bog DOM to smaller LMWcompounds and to DIC was inferred from lossesof UV absorbance, optical indices of molecularweight and changes in DOM chemistry. Experimentally, we observed a 1.2 enrichment in ¹³$C and a 47% loss in aromaticC functionality in bog DOM samples exposed tosolar UVR. Similar results were observed inthe surface waters of both lakes. In latesummer hypolimnetic water in humic LakeLacawac, we observed 3 to 4.5enrichments in ¹³C and a 30% increase inaromatic C relative to early spring valuesduring spring mixing. These changes coincidedwith increases in molecular weight and UVabsorbance. Anaerobic conditions of thehypolimnion in Lake Lacawac suggest thatmicrobial metabolism may be turning overallochthonous C introduced during springmixing, as well as autochthonous C. Thismetabolic activity produces HMW DOM during thesummer, which is photochemically labile andisotopically distinct from allochthonous DOM orautochthonous DOM. These results suggest bothphotooxidation of allochthonous DOM in theepilimnion and autotrophic production of DOM bybacteria in the hypolimnion cause seasonaltrends in the UV absorbance of lakes.     

Land Use History Mediates Soil Biogeochemical Responses to Drought in Temperate Forest Ecosystems

Ecosystems - Terrestrial ecosystems are experiencing increasing frequency and intensity of droughts as a result of climate change. Despite a wealth of previous studies investigating soil responses...     

鼎湖山黄果厚壳桂群落物种养分相似性研究

应用主分量分析与聚类分析技术, 测定鼎湖山黄果厚壳桂群落不同种群的养分相似性, 并探讨了植物不同器官作为相似性测度的合理性。并认为, 茎叶较适于作为相似性测试的取样部位。测定结果表明, 主分量分析与聚类分析对茎叶样本的测定结果具有一致性, 在主分量分析中, 茎第一排序轴主要反映Ｎ、Ｐ和Ｋ 元素, 叶第一排序轴主要反映Ｋ和Ｍｇ 元素。上层乔木叶子具有较大的相似性, 下层种双盖蕨、柏拉木与薄叶梧桐的叶子具有独特的养分特征, 黄果厚壳桂不同层次的个体具有相似的特征, 即种内的不同层次个体的差别不大。双盖蕨与柏拉木的茎样与其它种最不相似, 它与叶子的测定结果相一致。黄果厚壳桂不同层次个体的茎互不相似, 反映出不同木质化程度产生的营养含量上的差异。聚类分析显示, 处于上层的荷木、椎栗与华润楠的茎最为相似, 它们与一、二层的黄果厚壳桂及鼎湖钓樟则在较高水平上聚合, 亦即相似性稍小, 但与其它种（ 或个体） 相比, 这５ 个种是较为相似的。     

丹参酮的药理作用研究进展

丹参酮在中药丹参中的含量较高,有较强的生理活性,是丹参主要有效成分之一。丹参酮的药理作用极其广泛,本文就丹参酮的抗肿瘤、抗动脉粥样硬化、抗心律失常、缩小心肌梗死面积、降低心肌耗氧量、逆转左心室肥厚、减轻缺血再灌注损伤、保肝脏及抗肝纤维化、保护心肌、改善微循环、抗菌消炎等作用进行综述,以便促进中药丹参在临床上广泛的应用。     

大鼠肝移植肝上下腔静脉不同缝合方式及手术体会

目的:探讨肝上下腔静脉不同缝合方式对大鼠原位肝移植的影响.方法:选取雄性SD大鼠60对,以SD大鼠为供体和受体,随机分成A、B两组,每组30对.两组均采用改良Kamada"二袖套法"进行大鼠原位肝移植,A组采用双定点连续缝合方式吻合肝上下腔静脉,B组采用单定点连续缝合方式吻合.比较两组肝上下腔静脉吻合时长、无肝期时长以...     

Pathogenicity studies on a bovine herpesvirus

A virus was isolated from uterine exudate of a cow and later identified as a herpesvirus that had no serologic relationship to several known viruses. The virus produced a febril response in 7 month old Holstein steers. Inoculation of the virus into bovine fetuses of various gestational ages resulted in death of some fetuses in the 3rd and 4th months of gestation. Some fetuses in the 3rd and 4th month, and all older than 4 months, survived the inoculation. These fetuses experienced a lymphoreticular activation and a humoral antibody response.