全文获取类型
收费全文 | 1257篇 |
免费 | 126篇 |
专业分类
1383篇 |
出版年
2021年 | 15篇 |
2020年 | 12篇 |
2019年 | 13篇 |
2018年 | 14篇 |
2017年 | 12篇 |
2016年 | 16篇 |
2015年 | 36篇 |
2014年 | 48篇 |
2013年 | 51篇 |
2012年 | 48篇 |
2011年 | 57篇 |
2010年 | 31篇 |
2009年 | 35篇 |
2008年 | 57篇 |
2007年 | 59篇 |
2006年 | 45篇 |
2005年 | 51篇 |
2004年 | 31篇 |
2003年 | 38篇 |
2002年 | 40篇 |
2001年 | 23篇 |
2000年 | 42篇 |
1999年 | 29篇 |
1998年 | 20篇 |
1997年 | 13篇 |
1996年 | 13篇 |
1995年 | 15篇 |
1994年 | 11篇 |
1993年 | 9篇 |
1992年 | 15篇 |
1991年 | 31篇 |
1990年 | 24篇 |
1989年 | 20篇 |
1988年 | 17篇 |
1987年 | 12篇 |
1986年 | 18篇 |
1985年 | 20篇 |
1984年 | 29篇 |
1983年 | 25篇 |
1982年 | 15篇 |
1981年 | 20篇 |
1980年 | 9篇 |
1979年 | 21篇 |
1978年 | 20篇 |
1977年 | 23篇 |
1976年 | 24篇 |
1975年 | 13篇 |
1974年 | 11篇 |
1973年 | 19篇 |
1972年 | 16篇 |
排序方式: 共有1383条查询结果,搜索用时 8 毫秒
91.
The Dictyostelium LIM domain-containing protein LIM2 is essential for proper chemotaxis and morphogenesis 下载免费PDF全文
Chien S Chung CY Sukumaran S Osborne N Lee S Ellsworth C McNally JG Firtel RA 《Molecular biology of the cell》2000,11(4):1275-1291
We have identified limB, a gene encoding a novel LIM domain-containing protein, LIM2, in a screen for genes required for morphogenesis. limB null cells aggregate, although poorly, but they are unable to undergo morphogenesis, and the aggregates arrest at the mound stage. limB null cells exhibit an aberrant actin cytoskeleton and have numerous F-actin-enriched microspikes. The cells exhibit poor adhesion to a substratum and do not form tight cell-cell agglomerates in suspension. Furthermore, limB null cells are unable to properly polarize in chemoattractant gradients and move very poorly. Expression of limB from a prestalk-specific but not a prespore-specific promoter complements the morphogenetic defects of the limB null strain, suggesting that the limB null cell developmental defect results from an inability to properly sort prestalk cells. LIM2 protein is enriched in the cortex of wild-type cells, although it does not colocalize with the actin cytoskeleton. Our analysis indicates that LIM2 is a new regulatory protein that functions to control rearrangements of the actin cytoskeleton and is required for cell motility and chemotaxis. Our findings may be generally applicable to understanding pathways that control cell movement and morphogenesis in all multicellular organisms. Structure function studies on the LIM domains are presented. 相似文献
92.
93.
94.
Effects of species and habitat positional errors on the performance and interpretation of species distribution models 总被引:1,自引:0,他引:1
Aim A key assumption in species distribution modelling is that both species and environmental data layers contain no positional errors, yet this will rarely be true. This study assesses the effect of introduced positional errors on the performance and interpretation of species distribution models. Location Baixo Alentejo region of Portugal. Methods Data on steppe bird occurrence were collected using a random stratified sampling design on a 1‐km2 pixel grid. Environmental data were sourced from satellite imagery and digital maps. Error was deliberately introduced into the species data as shifts in a random direction of 0–1, 2–3, 4–5 and 0–5 pixels. Whole habitat layers were shifted by 1 pixel to cause mis‐registration, and the cumulative effect of one to three shifted layers investigated. Distribution models were built for three species using three algorithms with three replicates. Test models were compared with controls without errors. Results Positional errors in the species data led to a drop in model performance (larger errors having larger effects – typically up to 10% drop in area under the curve on average), although not enough for models to be rejected. Model interpretation was more severely affected with inconsistencies in the contributing variables. Errors in the habitat layers had similar although lesser effects. Main conclusions Models with species positional errors are hard to detect, often statistically good, ecologically plausible and useful for prediction, but interpreting them is dangerous. Mis‐registered habitat layers produce smaller effects probably because shifting entire layers does not break down the correlation structure to the same extent as random shifts in individual species observations. Spatial autocorrelation in the habitat layers may protect against species positional errors to some extent but the relationship is complex and requires further work. The key recommendation must be that positional errors should be minimised through careful field design and data processing. 相似文献
95.
The ability to flexibly produce facial expressions and vocalizations has a strong impact on the way humans communicate, as it promotes more explicit and versatile forms of communication. Whereas facial expressions and vocalizations are unarguably closely linked in primates, the extent to which these expressions can be produced independently in nonhuman primates is unknown. The present work, thus, examined if chimpanzees produce the same types of facial expressions with and without accompanying vocalizations, as do humans. Forty-six chimpanzees (Pan troglodytes) were video-recorded during spontaneous play with conspecifics at the Chimfunshi Wildlife Orphanage. ChimpFACS was applied, a standardized coding system to measure chimpanzee facial movements, based on FACS developed for humans. Data showed that the chimpanzees produced the same 14 configurations of open-mouth faces when laugh sounds were present and when they were absent. Chimpanzees, thus, produce these facial expressions flexibly without being morphologically constrained by the accompanying vocalizations. Furthermore, the data indicated that the facial expression plus vocalization and the facial expression alone were used differently in social play, i.e., when in physical contact with the playmates and when matching the playmates’ open-mouth faces. These findings provide empirical evidence that chimpanzees produce distinctive facial expressions independently from a vocalization, and that their multimodal use affects communicative meaning, important traits for a more explicit and versatile way of communication. As it is still uncertain how human laugh faces evolved, the ChimpFACS data were also used to empirically examine the evolutionary relation between open-mouth faces with laugh sounds of chimpanzees and laugh faces of humans. The ChimpFACS results revealed that laugh faces of humans must have gradually emerged from laughing open-mouth faces of ancestral apes. This work examines the main evolutionary changes of laugh faces since the last common ancestor of chimpanzees and humans. 相似文献
96.
Aaron M. Dickey Vivek Kumar Mark S. Hoddle Joe E. Funderburk J. Kent Morgan Antonella Jara-Cavieres Robert G. Jr. Shatters Lance S. Osborne Cindy L. McKenzie 《PloS one》2015,10(4)
Invasive arthropods pose unique management challenges in various environments, the first of which is correct identification. This apparently mundane task is particularly difficult if multiple species are morphologically indistinguishable but accurate identification can be determined with DNA barcoding provided an adequate reference set is available. Scirtothrips dorsalis is a highly polyphagous plant pest with a rapidly expanding global distribution and this species, as currently recognized, may be comprised of cryptic species. Here we report the development of a comprehensive DNA barcode library for S. dorsalis and seven nuclear markers via next-generation sequencing for identification use within the complex. We also report the delimitation of nine cryptic species and two morphologically distinguishable species comprising the S. dorsalis species complex using histogram analysis of DNA barcodes, Bayesian phylogenetics, and the multi-species coalescent. One member of the complex, here designated the South Asia 1 cryptic species, is highly invasive, polyphagous, and likely the species implicated in tospovirus transmission. Two other species, South Asia 2, and East Asia 1 are also highly polyphagous and appear to be at an earlier stage of global invasion. The remaining members of the complex are regionally endemic, varying in their pest status and degree of polyphagy. In addition to patterns of invasion and endemism, our results provide a framework both for identifying members of the complex based on their DNA barcode, and for future species delimiting efforts. 相似文献
97.
This paper addresses 123I and 125I dual isotope SPECT imaging, which can be challenging because of spectrum overlap in the low energy spectrums of these isotopes. We first quantify the contribution of low-energy photons from each isotope using GATE-based Monte Carlo simulations for the MOBY mouse phantom. We then describe and analyze a simple, but effective method that uses the ratio of detected low and high energy 123I activity to separate the mixed low energy 123I and 125I activities. Performance is compared with correction methods used in conventional tissue biodistribution techniques. The results indicate that the spectrum overlap effects can be significantly reduced, if not entirely eliminated, when attenuation and scatter is either absent or corrected for using standard methods. In particular, we show that relative activity levels of the two isotopes can be accurately estimated for a wide range of organs and provide quantitative validation that standard methods for spectrum overlap correction provide reasonable estimates for reasonable corrections in small-animal SPECT/CT imaging. 相似文献
98.
Vivek Kumar Dakshina R. Seal Lance S. Osborne Cindy L. McKenzie 《Applied Entomology and Zoology》2014,49(3):403-409
The small size and cryptic nature of thrips pests often make their monitoring and the identification process difficult. With the aim to obtain accurate identification of various pest species in the order Thysanoptera, a pilot study was conducted on an invasive thrips species Scirtothrips dorsalis Hood in Florida. The specific objective of this pilot study was to assess if the thrips specimens processed to be observed under scanning electron microscopy (SEM) can be further utilized for DNA based assays. Larvae and adults of S. dorsalis were subjected to traditional morphological identification using high resolution SEM prior to their DNA extraction. Sequence results of both mtCO1 and ITS rDNA of individual larva and adult S. dorsalis were found to be in agreement with the taxonomic identification conducted using SEM and each result confirmed the other technique. Our results suggest that steps involved during specimen preparation and observation under SEM does not impact DNA analysis of the sample. The two techniques together could be used for the correct identification of various thrips species using the same specimen. The proof of concept was further tested on three other important thrips species Frankliniella occidentalis (Pergande), F. schultzei (Trybom) and Thrips palmi Karny, which confirmed the robustness of the technique. The technique has broader significance for different areas under entomological sciences especially medical, forensic and taxonomic studies where the samples put under a high beam of ions for investigation are often required for DNA analysis. 相似文献
99.
David C. Blakemore Justin S. Bryans Pauline Carnell Christopher L. Carr Nicola E.A. Chessum Mark J. Field Natasha Kinsella Simon A. Osborne Andrew N. Warren Sophie C. Williams 《Bioorganic & medicinal chemistry letters》2010,20(2):461-464
Synthesis of a number of bicyclic five-membered ring derivatives of gabapentin led to the identification of two compounds, (?)-(11A) and (20A) which both had an excellent level of potency against α2δ and were profiled in an in vivo model of neuropathic pain. 相似文献
100.
R. Michael Lehman Shannon L. Osborne Deirdre A. Prischmann-Voldseth Kurt A. Rosentrater 《Plant and Soil》2010,333(1-2):481-490
The relative decomposability of corn (Zea mays L.) residues from insect (Bt)-protected hybrids and conventional hybrids cultivated under insect pressure was investigated in two studies. Above-ground biomass, residue macromolecular composition, and stalk physical strength were also measured. In the first decomposition study, chopped residues (stalks and leaves) were used from a corn rootworm-protected (Cry3Bb1) hybrid and its non-Bt near isoline that were grown in replicated plots infested with corn rootworms (Diabrotica spp.). In the second study, residue (intact stalk sections) was used from three European corn borer (ECB, Ostrinia nubilalis Hübner)-resistant (Cry1Ab) hybrids representing different seed manufacturer/maturity date series, their non-Bt near isolines, two Cry3Bb1-protected isolines, and three additional conventional hybrids, all cultivated in replicated plots under conditions of elevated ECB pressure. In both studies, insect-resistant residues decomposed at rates similar to their non-protected near isolines. No evidence was found that insect-protected hybrids produced more above-ground biomass or had distinct residue composition. While some measures of mechanical stalk strength indicated that ECB-damaged stalks were not as stiff as protected stalks, these physical differences did not translate into differences in residue decomposition. We conclude that while individual hybrids may vary in their production of biomass, residue composition or residue decomposability, these characteristics do not systematically vary with the presence of the Bt gene conferring insect resistance, even under conditions of insect pressure. 相似文献