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101.
102.
Stoll L Hall J Van Buren N Hall A Knight L Morgan A Zuger S Van Deusen H Gentile L 《Biophysical journal》2007,92(4):1343-1349
Ionotropic glutamate receptors (iGluRs), a family of ligand-gated ion channels, are responsible for the majority of fast excitatory neurotransmission in the central nervous system. Within this family, different members serve distinct roles at glutamatergic synapses. Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors mediate fast depolarization while N-methyl-D-aspartate (NMDA) receptors mediate the slower component of the excitatory postsynaptic potential. These disparate functions suggest alternate modes of regulation. In this work, we show that endogenous regulators of iGluRs have different abilities to bind to specific domains of NMDA NR1-1b and AMPA GluR2 subunits. We have previously shown that the sulfated neurosteroids pregnenolone sulfate and 3α-hydroxy-5β-pregnan-20-one sulfate bind to the extracellular glutamate-binding core (S1S2) of the GluR2 subunit. Here we show that neither neurosteroid binds to the S1S2 domain of the NMDA NR1-1b subunit. This NR1-1b NMDA domain does, however, bind to the endogenous polyamines spermine and spermidine as well as Zn(II). Binding of the polyamines and Zn(II) to the S1S2 domain of the GluR2 subunit was not observed. This binding of Zn(II) and polyamines to the S1S2 domain of the NR1-1b subunit defines a new binding site for each of these modulators. 相似文献
103.
Ben-Dov E Shapiro OH Siboni N Kushmaro A 《Applied and environmental microbiology》2006,72(11):6902-6906
To overcome the shortcomings of universal 16S rRNA gene primers 8F and 907R when studying the diversity of complex microbial communities, the 3' termini of both primers were replaced with inosine. A comparison of the clone libraries derived using both primer sets showed seven bacterial phyla amplified by the altered primer set (8F-I/907R-I) whereas the original set amplified sequences belonging almost exclusively to Proteobacteria (95.8%). Sequences belonging to Firmicutes (42.6%) and Thermotogae (9.3%) were more abundant in a library obtained by using 8F-I/907R-I at a PCR annealing temperature of 54 degrees C, while Proteobacteria sequences were more frequent (62.7%) in a library obtained at 50 degrees C, somewhat resembling the result obtained using the original primer set. The increased diversity revealed by using primers 8F-I/907R-I confirms the usefulness of primers with inosine at the 3' termini in studying the microbial diversity of environmental samples. 相似文献
104.
Nonalcoholic fatty liver disease and measures of early brain health in middle‐aged adults: The CARDIA study 下载免费PDF全文
105.
Rune Dietz Mads Peter Heide-Jørgensen Pierre Richard Jack Orr Kristin Laidre Hans Christian Schmidt 《Polar Biology》2008,31(11):1295-1306
Twenty-one narwhals tagged in 2003 and 2004 in Admiralty Inlet showed a different summer distributional pattern than previous
narwhal-tracking studies from Somerset Island, Eclipse Sound and Melville Bay. The migration of the narwhals tracked from
Admiralty Inlet moved out through Lancaster Sound 15 days earlier (P < 0.0001) than the narwhals summering around Eclipse Sound, whereas the Admiralty Inlet narwhals reached the mouths of Eclipse
Sound 18 days later (P < 0.0001) than the Eclipse Sound summering population. The winter range of the Admiralty Inlet narwhals overlapped with the
winter range of narwhals from Melville Bay and Eclipse Sound in central southern Baffin Bay and Northern Davis Strait, but
not with the winter range of narwhals from Somerset Island that wintered further north. Distribution size of range, and population
size did not appear to be related. An example of considerable year to year variation between area of summer and winter distribution
in the 2 years was believed to be related to the sample size and number of pods of whales tagged, rather than to differences
in sex or age classes. 相似文献
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Eitan Ben-Dov Nachshon Siboni Orr H. Shapiro Luba Arotsker Ariel Kushmaro 《Microbial ecology》2011,61(1):1-6
Universal 16S rRNA gene primers (8F and 518R) bearing inosine substitutions at either the 3??-ultimate or the 3??-ultimate and penultimate base positions were exploited for the first time to study the bacterial community associated with coral polymicrobial Black Band Disease (BBD). Inosine-modified universal primer pairs display some shifting in the composition of 16S rRNA gene libraries, as well as expanding the observed diversity of a BBD bacterial community at the family/class level. Possible explanations for the observed shifts are discussed. These results thus point to the need for adopting multiple approaches in designing 16S rRNA universal primers for PCR amplification and subsequent construction of 16S rRNA gene libraries or pyrosequencing in the exploration of complex microbial communities. 相似文献