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981.
By a proteomic approach, we demonstrated in rat coagulating gland secretion the presence of a 120 kDa protein which shares at least 80% identity at the amino acid level with the most closely related kinesin heavy chain codified by the kinesin superfamily protein Kif5c gene. In addition, we identified 30 and 66 kDa proteolytic fragments of such a kinesin heavy chain-like protein, corresponding to the 73-299 N-terminal and 300-860 C-terminal regions, respectively. Finally, we demonstrated the occurrence in coagulating gland secretion of a 200 kDa protein probably derived by cross-linking reaction of the kinesin heavy chain-like protein with type IV transglutaminase. In fact, kinesin heavy chain-like protein and its 66 kDa proteolytic fragment were also found to act as effective acyl donor substrates for the enzyme in vitro. 相似文献
982.
The New Zealand flatworm (Arthurdendyus triangulatus), which is an obligate predator of native earthworms, is an alien species to the British Isles and is widely distributed in Scotland. What little is known about its biology under field conditions is mainly from Northern Ireland. Samples taken from single sampling dates have shown A. triangulatus to have an aggregated distribution. To determine the spatio‐temporal distribution of A. triangulatus, a grass field in western Scotland was intensively sampled over a 16‐month period. Data indicate an increase in flatworm numbers and seasonal trends in body weight, the appearance of egg capsules and hatchlings. Results also showed that spatially, the flatworms were aggregated, but this was a transient phenomenon over the period of the experiment. The distribution of egg capsules in the study area was strongly aggregated and related to the appearance of hatchlings. Flatworms may aggregate in areas where soil moisture is optimal for the survival of the New Zealand flatworm. 相似文献
983.
Though epidemiology dates back to the 1700s, most mathematical representations of epidemics still use transmission rates averaged at the population scale, especially for wildlife diseases. In simplifying the contact process, we ignore the heterogeneities in host movements that complicate the real world, and overlook their impact on spatiotemporal patterns of disease burden. Movement ecology offers a set of tools that help unpack the transmission process, letting researchers more accurately model how animals within a population interact and spread pathogens. Analytical techniques from this growing field can also help expose the reverse process: how infection impacts movement behaviours, and therefore other ecological processes like feeding, reproduction, and dispersal. Here, we synthesise the contributions of movement ecology in disease research, with a particular focus on studies that have successfully used movement‐based methods to quantify individual heterogeneity in exposure and transmission risk. Throughout, we highlight the rapid growth of both disease and movement ecology and comment on promising but unexplored avenues for research at their overlap. Ultimately, we suggest, including movement empowers ecologists to pose new questions, expanding our understanding of host–pathogen dynamics and improving our predictive capacity for wildlife and even human diseases. 相似文献
984.
What determines prey selection in owls? Roles of prey traits,prey class,environmental variables,and taxonomic specialization
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Ecological theory suggests that prey size should increase with predator size, but this trend may be masked by other factors affecting prey selection, such as environmental constraints or specific prey preferences of predator species. Owls are an ideal case study for exploring how predator body size affects prey selection in the presence of other factors due to the ease of analyzing their diets from owl pellets and their widespread distributions, allowing interspecific comparisons between variable habitats. Here, we analyze various dimensions of prey resource selection among owls, including prey size, taxonomy (i.e., whether or not particular taxa are favored regardless of their size), and prey traits (movement type, social structure, activity pattern, and diet). We collected pellets of five sympatric owl species (Athene noctua, Tyto alba, Asio otus, Strix aluco, and Bubo bubo) from 78 sites across the Mediterranean Levant. Prey intake was compared between sites, with various environmental variables and owl species as predictors of abundance. Despite significant environmental impacts on prey intake, some key patterns emerge among owl species studied. Owls select prey by predator body size: Larger owls tend to feed on wider ranges of prey sizes, leading to higher means. In addition, guild members show both specialization and generalism in terms of prey taxa, sometimes in contrast with the expectations of the predator–prey body size hypothesis. Our results suggest that while predator body size is an important factor in prey selection, taxon specialization by predator species also has considerable impact. 相似文献
985.
Maxime W.C. Rousseaux Tyler Tschumperlin Hsiang-Chih Lu Elizabeth P. Lackey Vitaliy V. Bondar Ying-Wooi Wan Qiumin Tan Carolyn J. Adamski Jillian Friedrich Kirk Twaroski Weili Chen Jakub Tolar Christine Henzler Ajay Sharma Aleksandar Bajić Tao Lin Lisa Duvick Zhandong Liu Harry T. Orr 《Neuron》2018,97(6):1235-1243.e5
986.
Overexpression of antimicrobial peptides contributes to aging through cytotoxic effects in Drosophila tissues
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987.
A real-time PCR assay was used to quantify the relationship in alfalfa and pea between disease severity and the amount of Aphanomyces euteiches detected in roots. The study included isolates of race 1 and race 2 of the alfalfa pathovar of A. euteiches and an isolate obtained from diseased pea. Spearman rank correlations between pathogen DNA content and disease severity index (DSI) ratings were positive ( ? 0.57) and significant (P 0.0007) for individual alfalfa plants, bulked alfalfa plant samples, and individual pea plants. In all experiments, significantly more pathogen was detected in susceptible populations than in resistant populations. The results clearly demonstrate that resistance to A. euteiches in both alfalfa and pea is characterized by a reduction in pathogen colonization relative to levels observed for susceptible reactions. The assay was very specific for A. euteiches, producing very linear assays with DNA extracted from pathogen isolates obtained from alfalfa, pea, and bean. Possible applications of the assay in conjunction with other real-time PCR assays specific to other legume pathogens are discussed in relation to simultaneous disease screening for multiple plant pathogens and the study of microbial population dynamics in mixed plant infections. 相似文献
988.
Marissa A. LeBlanc Lynette S. Penney Daniel Gaston Yuhao Shi Erika Aberg Mathew Nightingale Haiyan Jiang Roxanne M. Gillett Somayyeh Fahiminiya Christine Macgillivray Ellen P. Wood Philip D. Acott M. Naeem Khan Mark E. Samuels Jacek Majewski Andrew Orr Christopher R. McMaster Karen Bedard 《Human genetics》2013,132(11):1223-1234
Pediatric intracranial calcification may be caused by inherited or acquired factors. We describe the identification of a novel rearrangement in which a downstream pseudogene translocates into exon 9 of OCLN, resulting in band-like brain calcification and advanced chronic kidney disease in early childhood. SNP genotyping and read-depth variation from whole exome sequencing initially pointed to a mutation in the OCLN gene. The high degree of identity between OCLN and two pseudogenes required a combination of multiplex ligation-dependent probe amplification, PCR, and Sanger sequencing to identify the genomic rearrangement that was the underlying genetic cause of the disease. Mutations in exon 3, or at the 5–6 intron splice site, of OCLN have been reported to cause brain calcification and polymicrogyria with no evidence of extra-cranial phenotypes. Of the OCLN splice variants described, all make use of exon 9, while OCLN variants that use exons 3, 5, and 6 are tissue specific. The genetic rearrangement we identified in exon 9 provides a plausible explanation for the expanded clinical phenotype observed in our individuals. Furthermore, the lack of polymicrogyria associated with the rearrangement of OCLN in our patients extends the range of cranial defects that can be observed due to OCLN mutations. 相似文献
989.