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61.
Based on materials of trawl studies performed from 1992 to 2002 off the Pacific coast of Kamchatka and the northern Kuril
Islands in the framework of the integrated (VNIRO, KamchatNIRO, Sakh-NIRO) program on the study of fishery resources of the
continental slope, data on the distribution and biology of rock greenling Hexagrammos lagocephalus were analyzed. It was elucidated that this species is extremely eurybathic; the range of its seasonal vertical migrations
embraces depths of 1–2 m in summer, and in the spawning period and up to 665 m in winter. The spatial distribution has a mosaic
pattern, maximum concentrations were recorded at the slope and shelf of the Shumshu Island and the southern extremity of Kamchatka.
The size, weight, and sex composition, and the maturation and feeding of the rock greenling are considered. Maximum fish sizes
in trawl catches are 58 cm and 2.63 kg; sex composition is characterized by the domination of females in all size groups (79%).
The bulk of feeding consisted of Cephalopoda, small crustaceans—Amphipoda and Isopoda—as well as the eggs of fish and Gastropoda.
With an increase in sizes, the greenling passes from feeding on small benthic organisms to larger movable forms. 相似文献
62.
E E Vitiaev Iu L Orlov O V Vishnevski? A S Belenok N A Kolchanov 《Molekuliarnaia biologiia》2001,35(6):952-960
A method is proposed to automatically search for patterns in the mutual location of context signals in regulatory DNA sequences. The procedure is based on the methods of Data Mining and Knowledge Discovery software, implemented in a computer system Gene Discovery. This system was used to study erythroid-specific promoters and promoters of the endocrine-system genes from TRRD. We detected some trends in occurrence and localization of specific oligonucleotide groups. 相似文献
63.
64.
FRIZZY PANICLE Drives Supernumerary Spikelets in Bread Wheat 总被引:1,自引:0,他引:1
Oxana Dobrovolskaya Caroline Pont Richard Sibout Petr Martinek Ekaterina Badaeva Florent Murat Audrey Chosson Nobuyoshi Watanabe Elisa Prat Nadine Gautier Véronique Gautier Charles Poncet Yuriy L. Orlov Alexander A. Krasnikov Hélène Bergès Elena Salina Lyudmila Laikova Jerome Salse 《Plant physiology》2015,167(1):189-199
65.
66.
N. Ya. Orlov T. G. Orlova A. R. Nezvetsky E. A. Burstein H. D. Bartunik 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2011,5(2):121-127
cGMP-Specific phosphodiesterase (PDE6) is the key enzyme of the phototransduction system of vertebrate retinal rod outer segments
(ROS). The properties of PDE in extracts prepared by solubilization of bovine ROS in a high concentration (0.5% w/v) of detergent
n-nonyl-β-D-glucoside (NG) and following centrifugation (ROS-NG) have been studied. Basal PDE activity of the preparations
was low, but it greatly (>50-fold) increased (up to ∼20 μmol cGMP hydrolyzed/min per mg rhodopsin (R)) in the presence of
trypsin. In bleached GTPγS-containing preparations the specific PDE activity was dependent on ROS-NG concentration and was
half-maximal at about 0.8 μM of ROS G protein transducin (Gt). In dark-adapted GTPγS-containing ROS-NG preparations bleaching
of 0.2% of the rhodopsin resulted in half-maximal PDE activation. The same result was obtained when PDE in dark-adapted ROS-NG
preparations was activated by addition of a highly purified bleached rhodopsin solubilized by 0.5% solution of NG. The results
demonstrate that the presence of NG has no significant influence either on the properties of the main ROS phototrans-duction
system elements (R, Gt and PDE) or on the interaction between photoactivated R and Gt and suggest that the detergent NG can
be used for crystallization of the rhodopsin-transducin complex. 相似文献
67.
68.
N Thorin-Trescases S N Orlov S Taurin N O Dulin B G Allen D deBlois J Tremblay A V Pshezhetsky P Hamet 《Canadian journal of physiology and pharmacology》2001,79(6):471-480
The effect of cholera toxin (CTX), an activator of the adenylate cyclase-coupled G protein alpha(s) subunit, was studied on cultured vascular smooth muscle cell (VSMC) proliferation. Continuous exposure (48 h) to CTX as well as 2-min pretreatment of VSMC with CTX led to the same level of cAMP production, inhibition of DNA synthesis, and arrest in the G1 phase without induction of necrosis or apoptosis in VSMC. Protein kinase A (PKA) activity in CTX-pretreated cells was transiently elevated by 3-fold after 3 h of incubation, whereas after 48 h it was reduced by 2-fold compared with baseline values without modulation of the expression of its catalytic alpha subunit. The PKA inhibitors H89 and KT 5720 did not protect VSMC from the antiproliferative effect of CTX. Two-dimensional electrophoresis was used to analyze the influence of CTX on protein phosphorylation. After 3 h of incubation of CTX-pretreated cells, we observed both newly-phosphorylated and dephosphorylated proteins (77 and 50 protein species, respectively). After 24 h of incubation, the number of phosphorylated proteins in CTX-treated cells was decreased to 39, whereas the number of dephosphorylated proteins was increased to 106. In conclusion, brief exposure to CTX leads to full-scale activation of cAMP signaling and evokes VSMC arrest in the G1 phase. 相似文献
69.
Kinetic rates of antibody binding correlate with neutralization sensitivity of variant simian immunodeficiency virus strains
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Steckbeck JD Orlov I Chow A Grieser H Miller K Bruno J Robinson JE Montelaro RC Cole KS 《Journal of virology》2005,79(19):12311-12320
Increasing evidence suggests that an effective AIDS vaccine will need to elicit both broadly reactive humoral and cellular immune responses. Potent and cross-reactive neutralization of simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1) by polyclonal and monoclonal antibodies is well documented. However, the mechanisms of antibody-mediated neutralization have not been defined. The current study was designed to determine whether the specificity and quantitative properties of antibody binding to SIV envelope proteins correlate with neutralization. Using a panel of rhesus monoclonal antibodies previously characterized for their ability to bind and neutralize variant SIVs, we compared the kinetic rates and affinity of antibody binding to soluble envelope trimers by using surface plasmon resonance. We identified significant differences in the kinetic rates but not the affinity of monoclonal antibody binding to the neutralization-sensitive SIV/17E-CL and neutralization-resistant SIVmac239 envelope proteins that correlated with the neutralization sensitivities of the corresponding virus strains. These results suggest for the first time that neutralization resistance may be related to quantitative differences in the rates but not the affinity of the antibody-envelope interaction and may provide one mechanism for the inherent resistance of SIVmac239 to neutralization in vitro. Further, we provide evidence that factors in addition to antibody binding, such as epitope specificity, contribute to the mechanisms of neutralization of SIV/17E-CL in vitro. This study will impact the method by which HIV/SIV vaccines are evaluated and will influence the design of candidate AIDS vaccines capable of eliciting effective neutralizing antibody responses. 相似文献
70.
Akimova OA Bagrov AY Lopina OD Kamernitsky AV Tremblay J Hamet P Orlov SN 《The Journal of biological chemistry》2005,280(1):832-839
Recently, we reported that ouabain kills renal epithelial and vascular endothelial cells independently of elevation of the [Na(+)](i)/[K(+)](i) ratio. These observations raised the possibility of finding cardiotonic steroids (CTS) that inhibit the Na(+),K(+) pump without attenuating cell survival and vice versa. To test this hypothesis, we compared CTS action on Na(+),K(+) pump, [Na(+)](i) content, and survival of Madin-Darby canine kidney cells. At a concentration of 1 microM, ouabain and other tested cardenolides, as well as bufadienolides such as bufalin, cinobufagin, cinobufotalin, and telobufotoxin, led to approximately 10-fold inhibition of the Na(+),K(+) pump, a 2-3-fold decrease in staining with dimethylthiazol-diphenyltetrazolium (MTT), and massive death indicated by detachment of approximately 80% of cells and caspase-3 activation. In contrast, Na(+),K(+) pump inhibition and elevation of [Na(+)](i) seen in the presence of 3 microM marinobufagenin (MBG) and marinobufotoxin did not affect MTT staining and cell survival. Inhibition of the Na(+),Rb(+) pump in K(+)-free medium was not accompanied by a decline of MTT staining and cell detachment but increased sensitivity to CTS. In K(+)-free medium, half-maximal inhibition of (86)Rb influx was observed in the presence of 0.04 microM ouabain and 0.1 microM MBG, whereas half-maximal detachment and decline of MTT staining were detected at 0.03 and 0.004 microM of ouabain versus 10 and 3 microM of MBG, respectively. Both ouabain binding and ouabain-induced [Na(+)](i),[K(+)](i)-independent signaling were suppressed in the presence of MBG. Thus, our results show that CTS exhibit distinctly different potency in Na(+),K(+) pump inhibition and triggering of [Na(+)](i)/[K(+)](i)-independent signaling, including cell death. 相似文献