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121.
Aldo S. Pacheco Maria Teresa González Julie Bremner Marcelo Oliva Olaf Heilmayer Jürgen Laudien José M. Riascos 《Helgoland Marine Research》2011,65(3):413-424
Benthic communities show changes in composition and structure across different environmental characteristics and habitats.
However, incorporating species biological traits into the analysis can provide a better understanding of system functioning
within habitats. We compare the functional diversity of macrobenthic communities from a contrasting shallow (15 m) and deep
(50 m) sublittoral soft-sediment habitats in northern Chile, using biological traits analysis. Our aim was to highlight the
biological characteristics responsible for differences between habitats and the implications for ecosystem functioning. Trait
analysis showed that the deep habitat was restricted in providing functionally important biogenic structure and bioturbation
and supports less diverse feeding-related energy pathways. The shallow habitat is characterized by more diverse energy pathways
and a higher potential for matter exchange through bioturbation. We provide support to the predictions of transfer of energy
from the benthos to upper trophic levels in the shallow, which is characterized mainly by normoxia and little organic matter
content in the sediment. In the deep habitat, characterized by hypoxia and more organic matter, energy appears to be transferred
to microbial components. We suggest that trait analysis should be added to the traditional approaches based on species diversity,
because it provides indicators of ecosystem stress. 相似文献
122.
123.
Lara-Ramírez EE Segura-Cabrera A Guo X Yu G García-Pérez CA Rodríguez-Pérez MA 《PloS one》2011,6(2):e17300
Background
Helicobacter pylori has a reduced genome and lives in a tough environment for long-term persistence. It evolved with its particular characteristics for biological adaptation. Because several H. pylori genome sequences are available, comparative analysis could help to better understand genomic adaptation of this particular bacterium.Principal Findings
We analyzed nine H. pylori genomes with emphasis on microevolution from a different perspective. Inversion was an important factor to shape the genome structure. Illegitimate recombination not only led to genomic inversion but also inverted fragment duplication, both of which contributed to the creation of new genes and gene family, and further, homological recombination contributed to events of inversion. Based on the information of genomic rearrangement, the first genome scaffold structure of H. pylori last common ancestor was produced. The core genome consists of 1186 genes, of which 22 genes could particularly adapt to human stomach niche. H. pylori contains high proportion of pseudogenes whose genesis was principally caused by homopolynucleotide (HPN) mutations. Such mutations are reversible and facilitate the control of gene expression through the change of DNA structure. The reversible mutations and a quasi-panmictic feature could allow such genes or gene fragments frequently transferred within or between populations. Hence, pseudogenes could be a reservoir of adaptation materials and the HPN mutations could be favorable to H. pylori adaptation, leading to HPN accumulation on the genomes, which corresponds to a special feature of Helicobacter species: extremely high HPN composition of genome.Conclusion
Our research demonstrated that both genome content and structure of H. pylori have been highly adapted to its particular life style. 相似文献124.
Giusti L Cetani F Ciregia F Da Valle Y Donadio E Giannaccini G Banti C Pardi E Saponaro F Basolo F Berti P Miccoli P Pinchera A Marcocci C Lucacchini A 《Molecular bioSystems》2011,7(3):687-699
Parathyroid tumours are heterogeneous and in some cases the diagnosis may be difficult using histological features. In this study we used a two-dimensional electrophoresis (2D)/mass spectrometry (MS)-based approach to examine the global changes of parathyroid adenoma tissues protein profile compared to the parathyroid normal tissues. Validation of protein expression was performed by immunoblotting using specific antibodies. Ingenuity software was used to identify the biological processes to which these proteins belong and to construct a potential network. A total of 30 proteins were found to be differentially expressed, of which 22 resulted in being over-expressed. Proteins identified by 2D/MS/MS proteomics were classified into functional categories and a major change (≥ 2-fold) in terms of expression was found in proteins involved in response to biotic stimuli, cell organization and signal transduction. After Ingenuity analysis, 14-3-3 ζ/δ appears to be a key protein in the network of parathyroid adenoma, where it is linked to other proteins such as annexin A2, B box and SPRY domain-containing protein (BSPRY), p53 and epidermal growth factor receptor (EGFR). Our results suggest that the proteomic approach was able to differentiate the protein profiles of normal parathyroid and parathyroid adenoma and identify a panel of proteins which are differentially expressed. The functional role of these proteins in the network of intracellular pathways is discussed. 相似文献
125.
126.
Christine J Piek Willem Evert van Spil Greet Junius Aldo Dekker 《BMC veterinary research》2011,7(1):15
Background
Azathioprine is used as an immunosuppressant in canine immune-mediated hemolytic anemia (IMHA), but this potentially toxic and carcinogenic drug has not been proven to be beneficial. The aim of this study was to determine the difference in outcome and survival of dogs with idiopathic IMHA treated with a protocol that included azathioprine and prednisolone versus a protocol that included prednisolone alone. 相似文献127.
128.
129.
130.
Delayed leaf senescence in ethylene-deficient ACC-oxidase antisense tomato plants: molecular and physiological analysis 总被引:14,自引:3,他引:14
Isaac John Rachel Drake Aldo Farrell Wendy Cooper Pam Lee Peter Horton Don Grierson 《The Plant journal : for cell and molecular biology》1995,7(3):483-490
To determine the role of ethylene during tomato (Lycopersicon esculentum Mill. cv. Alisa Craig) leaf senescence, transgenic ACC oxidase antisense plants were analysed. Northern analysis of wild-type plants indicated that ACC oxidase mRNA accumulation normally begins in pre-senescent green leaves but was severely reduced in the antisense plants. Although the levels of ethylene evolved by wild-type and transgenic leaves increased during the progression of senescence, levels were extremely low in transgenic leaves. Leaf senescence, as assessed by colour change from green to yellow, was clearly delayed by 10–14 days in the antisense plants when compared with wild-type plants. Northern analysis of the photosynthesis-associated genes, cab and rbcS, indicated that levels of the corresponding mRNAs were higher in transgenic leaves which were not yet senescing compared with senescing wild-type leaves of exactly the same age. Northern analysis using probes for tomato fruit ripening-related genes expressed during leaf senescence indicated that once senescence was initiated the expression pattern of these mRNAs was similar in transgenic and wild-type leaves. In the antisense plants chlorophyll levels, photosynthetic capacity and chlorophyll fluorescence were higher when compared with senescing wild-type plants of the same age. Photosynthetic capacity and the quantum efficiency of photosystem II were maintained for longer in the transformed plants at values close to those observed in wild-type leaves prior to the visible onset of senescence. These results indicate that inhibiting ACC oxidase expression and ethylene synthesis results in delayed leaf senescence, rather than inducing a stay-green phenotype. Once senescence begins, it progresses normally. Onset of senescence is not, therefore, related to a critical level of ethylene. The correlation between higher levels prior to senescence and early onset, however, suggests that ethylene experienced by the plant may be a significant contributing factor in the timing of senescence. 相似文献