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21.
Gout S Marie C Lainé M Tavernier G Block MR Jacquier-Sarlin M 《Experimental cell research》2004,299(2):498-510
We have characterized the modulation of cell-cell adhesion and the structure of adherens junctions in the human colon adenocarcinoma HT-29 cell line that differentiates into enterocytes after glucose substitution for galactose in the medium. We demonstrate that differentiated cells (HT-29 Gal) rapidly established E-cadherin-mediated interactions in aggregation assays. This effect is not due to an increase in E-cadherin expression during this early stage of cell differentiation, but rather results from the maturation of preexisting adherens junctions. These junctions are characterized by the redistribution of E-cadherin to the basolateral membrane and its co-localization with the actin cytoskeleton. Subcellular fractionation studies indicate that actin-associated E-cadherins bind beta-catenin and p120ctn. Furthermore, the p120ctn/E-cadherin association is upregulated. These data reveal a cooperative interaction between p120ctn and E-cadherin that corresponds to mature functional adherens junctions able to initiate tight cell-cell adhesion required for epithelium architecture and further affirm the gatekeeper role of p120ctn. 相似文献
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Lejeune-Hénaut I Hanocq E Béthencourt L Fontaine V Delbreil B Morin J Petit A Devaux R Boilleau M Stempniak JJ Thomas M Lainé AL Foucher F Baranger A Burstin J Rameau C Giauffret C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(8):1105-1116
An understanding of the genetic determinism of frost tolerance is a prerequisite for the development of frost tolerant cultivars
for cold northern areas. In legumes, it is not known to which extent vernalization requirement or photoperiod responsiveness
are necessary for the development of frost tolerance. In pea (Pisum sativum L.) however, the flowering locus Hr is suspected to influence winter frost tolerance by delaying floral initiation until after the main winter freezing periods
have passed. The objective of this study was to dissect the genetic determinism of frost tolerance in pea by QTL analysis
and to assess the genetic linkage between winter frost tolerance and the Hr locus. A population of 164 recombinant inbred lines (RILs), derived from the cross Champagne x Terese was evaluated both
in the greenhouse and in field conditions to characterize the photoperiod response from which the allele at the Hr locus was inferred. In addition, the population was also assessed for winter frost tolerance in 11 field conditions. Six
QTL were detected, among which three were consistent among the different experimental conditions, confirming an oligogenic
determinism of frost tolerance in pea. The Hr locus was found to be the peak marker for the highest explanatory QTL of this study. This result supports the hypothesis
of the prominent part played by the photoperiod responsiveness in the determinism of frost tolerance for this species. The
consistency of three QTL makes these positions interesting targets for marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
25.
Alcântara-Neves NM dos Santos AB Mendonça LR Figueiredo CA Pontes-de-Carvalho L 《Experimental parasitology》2008,119(3):349-351
Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781-782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol. 相似文献
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María-Cruz Rodríguez José Barluenga Giuseppe Savona Franco Piozzi Orietta Servettaz Benjamin Rodriguez 《Phytochemistry》1984,23(7):1465-1469
A new neo-clerodane diterpenoid, isoteuflidin, was isolated from the aerial part of Teucrium chamaedrys. Its structure, 15,16-epoxy-3β-hydroxy-19 相似文献
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Giuseppe Savona Maurizio Bruno Franco Piozzi Orietta Servettaz Benjamín Rodríguez 《Phytochemistry》1984,23(4):849-852
From the aerial part of Teucrium massiliense three new neo-clerodane diterpenoids, deacetylajugarin-II, teumassilin and 6,19-diacetylteumassilin, have been isolated, besides the previously known diterpenes montanin C and teucjaponin A. The structures of deacetylajugarin-II (4α,18-epoxy-6α,19-dihydroxy-neo-clerodan-13-en-15,16-olide), teumassilin (4α,18:15,16-diepoxy-6α,12S,19-trihydroxy-neo-cleroda-13(16),14-diene) and 6,19-diacetylteumassilin (6α,19-diacetoxy-4α,18:15,16-diepoxy-12S-hydroxy-neo-cleroda-13(16),14-diene) were established by chemical and spectroscopic means. In addition, the previously known flavones salvigenin and cirsimaritin have also been obtained from the same source. 相似文献
28.
The implication of MAP kinases in the proliferation control of pancreatic cancer cells is still unknown. This study was undertaken to examine the contribution of the p44/p42 and p38 MAP kinases in the mitogenic response to epidermal growth factor (EGF) and bombesin in human pancreatic cancer cells, MIA PaCa-2 and PANC-1. Data indicate that EGF and bombesin stimulated growth of both cell lines. In MIA PaCa-2 cells, EGF and bombesin stimulated the in gel activation of p38 while p44/p42 kinases exhibited high basal activity and no response to stimuli. Growth and p38 activation were inhibited by genistein, wortmannin, PD98059 and SB203580, specific inhibitors of tyrosine kinase, phosphatidylinositol 3-kinase, MEK-1 and p38 kinases, respectively. In PANC-1 cells, EGF and bombesin stimulated p42 in gel activation; p44 remained highly activated and unresponsive to stimuli and p38 did not respond. Stimulated growth and p42 activation were inhibited by genistein, wortmannin and PD98059. Estimation of MAPK activities with a specific anti-active MAP kinase antibody indicated, however, that EGF increased the intensity of the bands corresponding to p42 and p44 MAP kinases in both cell lines, indicating that the mitogenic factor can regulate MAP kinase activity. Data also pointed out that ATP is sufficient to increase MAP kinase activity within the in gel assay technique and may thus explain the discrepancies existing between the in gel assay data and those obtained with the anti-active MAP kinase antibody. 相似文献
29.
Suppression and synthetic‐lethal genetic relationships of ΔgpsB mutations indicate that GpsB mediates protein phosphorylation and penicillin‐binding protein interactions in Streptococcus pneumoniae D39
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Britta E. Rued Jiaqi J. Zheng Andrea Mura Ho‐Ching T. Tsui Michael J. Boersma Jeffrey L. Mazny Federico Corona Amilcar J. Perez Daniela Fadda Linda Doubravová Karolína Buriánková Pavel Branny Orietta Massidda Malcolm E. Winkler 《Molecular microbiology》2017,103(6):931-957
GpsB regulatory protein and StkP protein kinase have been proposed as molecular switches that balance septal and peripheral (side‐wall like) peptidoglycan (PG) synthesis in Streptococcus pneumoniae (pneumococcus); yet, mechanisms of this switching remain unknown. We report that ΔdivIVA mutations are not epistatic to ΔgpsB division‐protein mutations in progenitor D39 and related genetic backgrounds; nor is GpsB required for StkP localization or FDAA labeling at septal division rings. However, we confirm that reduction of GpsB amount leads to decreased protein phosphorylation by StkP and report that the essentiality of ΔgpsB mutations is suppressed by inactivation of PhpP protein phosphatase, which concomitantly restores protein phosphorylation levels. ΔgpsB mutations are also suppressed by other classes of mutations, including one that eliminates protein phosphorylation and may alter division. Moreover, ΔgpsB mutations are synthetically lethal with Δpbp1a, but not Δpbp2a or Δpbp1b mutations, suggesting GpsB activation of PBP2a activity. Consistent with this result, co‐IP experiments showed that GpsB complexes with EzrA, StkP, PBP2a, PBP2b and MreC in pneumococcal cells. Furthermore, depletion of GpsB prevents PBP2x migration to septal centers. These results support a model in which GpsB negatively regulates peripheral PG synthesis by PBP2b and positively regulates septal ring closure through its interactions with StkP‐PBP2x. 相似文献
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