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871.
872.
The bioenergetic basis for the decrease in metabolic diversity at increasing salt concentrations: implications for the functioning of salt lake ecosystems 总被引:11,自引:1,他引:10
Aharon Oren 《Hydrobiologia》2001,466(1-3):61-72
Examination of the microbial diversity in hypersaline lakes of increasing salt concentrations shows that certain types of dissimilatory metabolism do not occur at the highest salinities. Examples are methanogenesis from hydrogen and carbon dioxide or from acetate, dissimilatory sulfate reduction with oxidation of acetate, and autotrophic nitrification. The observations can be explained on the basis of the energetic cost of haloadaptation used by the different metabolic groups and the free-energy change associated with the dissimilatory reactions. All halophilic microorganisms spend large amounts of energy to maintain steep gradients of Na+ and K+concentrations across their cytoplasmic membrane. Most Bacteria and also the methanogenic Archaea produce high intracellular concentrations of organic osmotic solutes at a high energetic cost. The halophilic aerobic Archaea (order Halobacteriales) and the halophilic fermentative Bacteria (order Halanaerobiales) use KCl as the main intracellular solute. This strategy, while requiring far-reaching adaptations of the intracellular machinery, is energetically more favorable than production of organic compatible solutes. By combining information on the amount of energy available to each physiological group and the strategy used to cope with salt stress, a coherent model emerges that provides explanations for the upper salinity limit at which the different microbial conversions occur in hypersaline lakes. 相似文献
873.
p53: the ultimate tumor suppressor gene? 总被引:24,自引:0,他引:24
M Oren 《FASEB journal》1992,6(13):3169-3176
Alterations in the gene encoding the cellular p53 protein are perhaps the most frequent type of genetic lesions in human cancer. At the heart of these alterations is the abrogation of the tumor suppressor activity of the normal p53. In many cases this is achieved through point mutations in p53, which often result in pronounced conformational changes. Such mutant polypeptides, which tend to accumulate to high levels in cancer cells, are believed to exert a dominant negative effect over coexpressed normal p53. Extensive research on p53, especially in the course of the last 3 years, has already provided much insight into the biological and biochemical mechanisms that underlie its capacity to act as a potent tumor suppressor. There are now many indications that p53 may play a central role in the control of cell proliferation, cell survival, and differentiation. Nevertheless, despite the purported importance of p53 for such crucial processes, mice can develop apparently without any defect in the total absence of p53. This raises the possibility that p53 may become critically limiting only when normal growth control is lost. 相似文献
874.
A basic biophysical model for bursting neurons 总被引:8,自引:0,他引:8
Presented here is a basic biophysical cell model for bursting, an extension of our previous model (Av-Ron et al. 1991) for excitability and oscillations. By changing a limited set of model parameters, one can describe different patterns of bursting behavior in terms of the burst cycle, the durations of oscillation and quiescence, and firing frequency. 相似文献
875.
876.
A. Oren 《Archives of microbiology》1996,165(5):354-358
Many members of the Halobacteriaceae are inhibited by quinolone compounds, which inhibit type II DNA topoisomerase. Ciprofloxacin
was the most potent inhibitor, followed by ofloxacin and norfloxacin. Ciprofloxacin concentrations between 25 and 60 μg/ml
caused 50% inhibition of the growth of most Haloferax and Haloarcula species. Halobacterium species were less sensitive. At sublethal concentrations, formation of elongated and/or swollen cells was observed in many
species. The alkaliphilic Natronobacterium pharaonis was very sensitive (50% inhibition by ciprofloxacin, ofloxacin, and norfloxacin at concentrations between 4 and 15 μg/ml).
The resistance of many members of the Halobacteriaceae to high concentrations of quinolone compounds may in part be due to
the high magnesium concentrations present in the growth media. Haloferax volcanii was sensitive to 40 μg/ml ciprofloxacin when grown at suboptimal magnesium concentrations (0.1 M), but was hardly affected
by 100 μg/ml of the inhibitor when grown in the presence of 0.5–0.75 M MgCl2. It is suggested that the putative archaeal type II DNA topoisomerase has properties similar to those of the enzyme from
Bacteria, although its sensitivity to quinolone antimicrobial compounds may be lower.
Received: 6 November 1995 / Accepted: 26 February 1996 相似文献
877.
878.
Sexual displays as amplifiers: practical examples with an emphasis on feather decorations 总被引:4,自引:1,他引:3
Exact patterns of displays are not easy to explain on a functionalbasis. However, if displays evolve initially as amplifiers ofpreviously perceived differences in quality, their patternsshould correspond with patterns of quality cues that are amplified.Consequently, exact patterns of behavioral, morphological, andstructural displays can be explained. I illustrate this withexamples of some feather decorations. Furthermore, these examplesindicate that, frequently, cues on which female choice is basedcannot easily run away, whereas their amplifiers can. Finally,I present specific predictions of the amplifying theory of sexualselection. 相似文献