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Mori Izumi C. Arias-Barreiro Carlos Raul Ooi Lia Lee Nam-Hee Sobahan Muhammad Abdus Nakamura Yoshimasa Hirai Yoshihiko Murata Yoshiyuki 《Journal of plant research》2021,134(5):1139-1148
Journal of Plant Research - It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic... 相似文献
514.
S. J. McInnes R. Tangney J. J. Brophy P. Thordarson M. K. J. Ooi 《Plant biology (Stuttgart, Germany)》2023,25(2):268-275
- Seed dormancy is the key driver regulating seed germination, hence is fundamental to the seedling recruitment life-history stage and population persistence. However, despite the importance of physical dormancy (PY) in timing post-fire germination, the mechanism driving dormancy-break within seed coats remains surprisingly unclear. We suggest that seed coat chemistry may play an important role in controlling dormancy in species with PY. In particular, seed coat fatty acids (FAs) are hydrophobic, and have melting points within the range of seed dormancy-breaking temperatures. Furthermore, melting points of saturated FAs increase with increasing carbon chain length. We investigated whether fire could influence seed coat FA profiles and discuss their potential influence on dormancy mechanisms.
- Seed coat FAs of 25 species within the Faboideae, from fire-prone and fire-free ecosystems, were identified and quantified through GC–MS. Fatty acid profiles were interpreted in the context of species habitat and interspecific variation.
- Fatty acid compositions were distinct between species from fire-prone and fire-free habitats. Fire-prone species tended to have longer saturated FA chains, a lower ratio of saturated to unsaturated FA, and a slightly higher relative amount of FAs compared to fire-free species.
- The specific FA composition of seed coats of fire-prone species indicated a potential role of FAs in dormancy mechanisms. Overall, the distinct FA composition between fire-prone and fire-free species suggests that chemistry of the seed coat may be under selection pressure in fire-prone ecosystems.
515.
Genes from Nine Genomes Are Separated into Their Organisms in the Dinucleotide Composition Space 总被引:1,自引:0,他引:1
A set of 16 kinds of dinucleotide compositions was used to analyzethe protein-encoding nucleotide sequences in nine complete genomes:Escherichia coli, Haemophilus influenzae, Helicobacter pylori,Mycoplasma genitalium, Mycoplasma pneumoniae, Synechocystissp., Methanococcus jannaschii, Archaeoglobus fulgidus, and Saccharomycescerevisiae. The dinucleotide composition was significantly differentbetween the organisms. The distribution of genes from an organismwas clustered around its center in the dinucleotide compositionspace. The genes from closely related organisms such as Gram-negativebacteria, mycoplasma species and eukaryotes showed some overlapin the space. The genes from nine complete genomes togetherwith those from human were discriminated into respective clusterswith 80% accuracy using the dinucleotide composition alone.The composition data estimated from a whole genome was closeto that obtained from genes, indicating that the characteristicfeature of dinucleotides holds not only for protein coding regionsbut also noncoding regions. When a dendrogram was constructedfrom the disposition of the clusters in the dinucleotide space,it resembled the real phylogenetic tree. Thus, the distinctfeature observed in the dinucleotide composition may reflectthe phylogenetic relationship of organisms. 相似文献
516.
Periodontal disease is associated with changes in the composition of the oral microflora, where health-associated oral streptococci decrease while Gram-negative anaerobes predominate in disease. A key feature of periodontal disease-associated anaerobes is their ability to produce hydrogen sulfide (H2S) abundantly as a by-product of anaerobic metabolism. So far, H2S has been reported to be either cytoprotective or cytotoxic by modulating bacterial antioxidant defense systems. Although oral anaerobes produce large amounts of H2S, the potential effects of H2S on oral streptococci are currently unknown. The aim of this study was to determine the effects of H2S on the survival and biofilm formation of oral streptococci. The growth and biofilm formation of Streptococcus mitis and Streptococcus oralis were inhibited by H2S. However, H2S did not significantly affect the growth of Streptococcus gordonii or Streptococcus sanguinis. The differential susceptibility of oral streptococci to H2S was attributed to differences in the intracellular concentrations of reduced glutathione (GSH). In the absence of GSH, H2S elicited its toxicity through an iron-dependent mechanism. Collectively, our results showed that H2S exerts antimicrobial effects on certain oral streptococci, potentially contributing to the decrease in health-associated plaque microflora. 相似文献
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The stability of the α-helices of isomeric block copolypeptides is nonequivalent, as reported previously. In order to explore the origin of the nonequivalence, the stability of α-helix of two block copolypeptides, (L -Ala)20-(L -Glu)20-(L -Phe) (designated as AEF) and (L -Glu)20-(L -Ala)20-(L -Phe) (EAF), in aqueous solution was investigated as a function of pH, temperature, and salt concentration by the measurement of the α-helical content using CD at 223 nm. The transition temperature, Tm, as a measure of the stability of the α-helix, decreased with increasing the salt concentration for EAF, while Tm increased for AEF. The results indicate that electrostatic interactions affect the nonequivalence of such helical stability. Thermodynamic quantities, ΔG, ΔH, and ΔS, of the thermal transition from random coil to α-helix were obtained by applying the curve-fitting method to the data. The major contribution to the effects of salts seems to be the entropic term, not the enthalpy term. This is unexpected, since the salt ions would weaken electrostatic interactions between ionized groups and the dipole along the helical axis, which affect the enthalpy term. In addition, the dependence of the electrostatic effect on the salt concentration is different for EAF and AEF. There fore, the nonequivalence cannot be accounted for by only the electrostatic effect, suggesting that it originates from some intrinsic property of the α-helix. 相似文献
519.
T Obinata A Ooi H Takano-Ohmuro 《Comparative biochemistry and physiology. B, Comparative biochemistry》1983,76(3):437-442
Myosin and actin were purified from ascidian smooth muscle. Ascidian myosin contained two classes of light chains and the pH dependence of Ca2+-activated ATPase and the KCl dependence of actin-activated ATPase of ascidian myosin differed from those of vertebrate skeletal myosin. Troponin-tropomyosin complex from ascidian increased the ATPase activity of ascidian reconstituted actomyosin in a Ca2+-dependent manner. Ascidian myosin provided the reconstituted actomyosin with the responsiveness to calcium ions. Two actin isoforms were present in ascidian, which were distinguished by isoelectric points. 相似文献
520.
W. Yamashita Y. Ito M. A. Weiss B. S. Ooi V. E. Pollak 《Prostaglandins, leukotrienes, and essential fatty acids》1988,34(3):161-166
To determine if the induction of immune-mediated glomerular injury influences the formation of glomerular cyclooxygenase products, we measured thromboxane B2 (TXB2), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) and prostaglandin E2 (PGE2) production by isolated glomeruli of rabbits induced with acute serum sickness nephritis by the administration of bovine serum ablumin (BSA). Animals were randomly assigned to one of three experimental groups: animals injected with BSA (BSA group; n = 11); animals injected with normal saline (control group; n = 11); and animals injected with BSA which were treated with the thromboxane synthetase inhibitor, OKY-046 (BSA + OKY-046; n = 6). Animals in the BSA and BSA + OKY groups developed severe proteinuria and glomerular histologic lesions of nephritis. No differences in proteinuria, serum creatinine and severity of histologic nephritis were observed between the two groups. Examination of glomerular eicosanoid production at the end of the experiment showed a marked reduction of glomerular PGE2 and 6-keto-PGF1 alpha production with a smaller reduction of glomerular TXB2 production in the BSA group. In the BSA + OKY-046 group, the production of TXB2 was significantly less than that in the BSA group; despite this, no effect on proteinuria could be discerned. 相似文献