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91.
Using food network unfolding to evaluate food–web complexity in terms of biodiversity: theory and applications 下载免费PDF全文
Yoshikazu Kato Michio Kondoh Naoto F. Ishikawa Hiroyuki Togashi Yukihiro Kohmatsu Mayumi Yoshimura Chikage Yoshimizu Takashi F. Haraguchi Yutaka Osada Nobuhito Ohte Naoko Tokuchi Noboru Okuda Takeshi Miki Ichiro Tayasu 《Ecology letters》2018,21(7):1065-1074
Food–web complexity often hinders disentangling functionally relevant aspects of food–web structure and its relationships to biodiversity. Here, we present a theoretical framework to evaluate food–web complexity in terms of biodiversity. Food network unfolding is a theoretical method to transform a complex food web into a linear food chain based on ecosystem processes. Based on this method, we can define three biodiversity indices, horizontal diversity (DH), vertical diversity (DV) and range diversity (DR), which are associated with the species diversity within each trophic level, diversity of trophic levels, and diversity in resource use, respectively. These indices are related to Shannon's diversity index (H′), where H′ = DH + DV ? DR. Application of the framework to three riverine macroinvertebrate communities revealed that D indices, calculated from biomass and stable isotope features, captured well the anthropogenic, seasonal, or other within‐site changes in food–web structures that could not be captured with H′ alone. 相似文献
92.
Nagasawa N Miyoshi M Sano Y Satoh H Hirano H Sakai H Nagato Y 《Development (Cambridge, England)》2003,130(4):705-718
We analyzed recessive mutants of two homeotic genes in rice, SUPERWOMAN1 (SPW1) and DROOPING LEAF (DL). The homeotic mutation spw1 transforms stamens and lodicules into carpels and palea-like organs, respectively. Two spw1 alleles, spw1-1 and spw1-2, show the same floral phenotype and did not affect vegetative development. We show that SPW1 is a rice APETALA3 homolog, OsMADS16. In contrast, two strong alleles of the dl locus, drooping leaf-superman1 (dl-sup1) and drooping leaf-superman2 (dl-sup2), cause the complete transformation of the gynoecium into stamens. In these strong mutants, many ectopic stamens are formed in the region where the gynoecium is produced in the wild-type flower and they are arranged in a non-whorled, alternate pattern. The intermediate allele dl-1 (T65), results in an increase in the number of stamens and stigmas, and carpels occasionally show staminoid characteristics. In the weakest mutant, dl-2, most of the flowers are normal. All four dl alleles cause midrib-less drooping leaves. The flower of the double mutant, spw1 dl-sup, produces incompletely differentiated organs indefinitely after palea-like organs are produced in the position where lodicules are formed in the wild-type flower. These incompletely differentiated organs are neither stamens nor carpels, but have partial floral identity. Based on genetic and molecular results, we postulate a model of stamen and carpel specification in rice, with DL as a novel gene controlling carpel identity and acting mutually and antagonistically to the class B gene, SPW1. 相似文献
93.
Satoshi Oguchi Hiroyuki Saito Masayoshi Tsukahara Haruhiko Tsumura 《Cytotechnology》2006,52(3):199-207
Controlling cell proliferation during cell culturing is an effective way to improve the production yield in mammalian cell
culture. We examined the effect of temperature shifts (TS) under pH control conditions in Chinese hamster ovary cells. When
we shifted the culture temperature from 37 °C to 31 °C before a stationary phase at pH 6.8 (TS/pH 6.8), cell viability remained
high, and the final human monoclonal antibody (hMab) concentration increased to 2.3 times that in the culture remaining at
37 °C. However, there were no significant effects on the cell viability or production yield with the same TS at pH 7.0 (TS/pH
7.0). The average specific hMab productivity and mRNA level of TS/pH 7.0 were the same as that of TS/pH 6.8. The control of
cell growth by the TS or the addition of rapamycin was effective in the maintenance of cell viability, but there was no significant
increase of the average specific hMab productivity in the culture where cell proliferation was controlled with rapamycin.
The hMab mRNA concentration decreased to 55%–65% at a 37 °C culture with the addition of actinomycin D. In contrast, actinomycin
D did not affect the mRNA level in the TS culture. This result suggested that the increase in the mRNA level in the TS condition
was caused by an increase in mRNA stability. In this study, we show that TS can produce two unrelated effects: a prolongation
of cell longevity and an improvement in mRNA stability. 相似文献
94.
9HODE stimulates cell proliferation and extracellular matrix synthesis in human mesangial cells via PPARgamma 总被引:2,自引:0,他引:2
Negishi M Shimizu H Okada S Kuwabara A Okajima F Mori M 《Experimental biology and medicine (Maywood, N.J.)》2004,229(10):1053-1060
Plasma oxidized low-density lipoprotein (OX-LDL) levels are elevated in patients with renal diseases, including diabetic nephropathy. We examined effects of OX-LDL on cell proliferation and extracellular matrix (ECM) production by using normal human mesangial cells. Furthermore, we examined possible involvement of peroxisome proliferator-activated receptor gamma (PPARgamma). Mesangial cell proliferation with OX-LDL, 9-hydroxy-10,12-octadecadienoic acid (9HODE), and 13-hydroxy-9,11-octadecadienoic acid (13HODE), the major components of OX-LDL, were determined by 5-bromo-2'-deoxyuridine (BrdU) or 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) incorporation. The effect of OX-LDL on mesangial cell proliferation with PD98059 pretreatment was determined by BrdU incorporation. Type IV collagen, fibronectin, and PPARgamma expression with OX-LDL or 9HODE or 13HODE was determined by Western blotting. Type IV collagen expression with antisense oligonucleotide against PPARgamma pretreatment was also determined by Western blotting. The effect of PD98059 pretreatment on PPARgamma expression was determined by Western blotting. In mesangial cells exposed to isolated OX-LDL from human plasma, BrdU incorporation was increased, and this increase was deleted by PD98059. Type IV collagen expression was significantly increased by OX-LDL. 9HODE and 13HODE increased BrdU and MTT incorporation into mesangial cells and also increased expressions of Type IV collagen and fibronection, the major components of ECM. PPARgamma expression in mesangial cells was stimulated by 9HODE. The reduction of PPARgamma synthesis by pretreatment of antisense oligonucleotide against PPARgamma remarkably attenuated Type IV collagen synthesis induced by 9HODE. PPARgamma expression induced by 9HODE was also reduced by PD98059 pretreatment. These findings demonstrate that 9HODE, the major component of OX-LDL, stimulates cell proliferation and ECM production of human mesangial cells. In addition, the stimulatory effects are, at least in part, mediated by PPARgamma, which may exist in downstream of ERK1/2 pathway. 相似文献
95.
Cyclin-dependent kinases (CDKs) play a central role in the regulation of cell cycle progression in eukaryotes. The onset of
S phase, the initiation of chromosomal DNA replication, is a major cell cycle event that is regulated by CDKs. Eukaryotic
chromosomal DNA replication is highly regulated and occurs as a two-step reaction. The first reaction, known as licensing,
is essential for DNA replication by making cell replication competent and occurs in G1 phase. Once cells enter S phase, licensed
chromosomes initiate DNA replication through the action of two conserved protein kinases, S phase-specific CDK and Cdc7-Dbf4
(or Dbf4-dependent kinase). Our understanding of the regulatory mechanisms of DNA replication in model eukaryotes has advanced
considerably in the past decade. In this review, we overview the regulation of DNA replication in the eukaryotic cell cycle,
focusing specifically on how CDKs regulate the initiation step of DNA replication. 相似文献
96.
Sato H Kato R Isogai Y Saka G Ohtsuki M Taketomi Y Yamamoto K Tsutsumi K Yamada J Masuda S Ishikawa Y Ishii T Kobayashi T Ikeda K Taguchi R Hatakeyama S Hara S Kudo I Itabe H Murakami M 《The Journal of biological chemistry》2008,283(48):33483-33497
Among the many mammalian secreted phospholipase A2 (sPLA2) enzymes, PLA2G3 (group III secreted phospholipase A2) is unique in that it possesses unusual N- and C-terminal domains and in that its central sPLA2 domain is homologous to bee venom PLA2 rather than to other mammalian sPLA2s. To elucidate the in vivo actions of this atypical sPLA2, we generated transgenic (Tg) mice overexpressing human PLA2G3. Despite marked increases in PLA2 activity and mature 18-kDa PLA2G3 protein in the circulation and tissues, PLA2G3 Tg mice displayed no apparent abnormality up to 9 months of age. However, alterations in plasma lipoproteins were observed in PLA2G3 Tg mice compared with control mice. In vitro incubation of low density (LDL) and high density (HDL) lipoproteins with several sPLA2s showed that phosphatidylcholine was efficiently converted to lysophosphatidylcholine by PLA2G3 as well as by PLA2G5 and PLA2G10, to a lesser extent by PLA2G2F, and only minimally by PLA2G2A and PLA2G2E. PLA2G3-modified LDL, like PLA2G5- or PLA2G10-treated LDL, facilitated the formation of foam cells from macrophages ex vivo. Accumulation of PLA2G3 was detected in the atherosclerotic lesions of humans and apoE-deficient mice. Furthermore, following an atherogenic diet, aortic atherosclerotic lesions were more severe in PLA2G3 Tg mice than in control mice on the apoE-null background, in combination with elevated plasma lysophosphatidylcholine and thromboxane A2 levels. These results collectively suggest a potential functional link between PLA2G3 and atherosclerosis, as has recently been proposed for PLA2G5 and PLA2G10. 相似文献
97.
Shinji Masuda Rei Ikeda Tatsuru Masuda Haruki Hashimoto Tohru Tsuchiya Hiroko Kojima Jiro Nomata Yuichi Fujita Mamoru Mimuro Hiroyuki Ohta Ken‐ichiro Takamiya 《The Plant journal : for cell and molecular biology》2009,58(6):952-960
In angiosperms, chlorophyll biosynthesis is light dependent. A key factor in this process is protochlorophyllide oxidoreductase (POR), which requires light to catalyze the reduction of protochlorophyllide to chlorophyllide. It is believed that this protein originated from an ancient cyanobacterial enzyme that was introduced into proto‐plant cells during the primary symbiosis. Here we report that PORs from the cyanobacteria Gloeobacter violaceus PCC7421 and Synechocystis sp. PCC6803 function in plastids. First, we found that the G. violaceus POR shows a higher affinity to its substrate protochlorophyllide than the Synechocystis POR but a similar affinity to plant PORs. Secondly, the reduced size of prolamellar bodies caused by a knockdown mutation of one of the POR genes, PORA, in Arabidopsis could be complemented by heterologous expression of the cyanobacterial PORs. Photoactive protochlorophyllide in the etioplasts of the complementing lines, however, was retained at a low level as in the parent PORA knockdown mutant, indicating that the observed formation of prolamellar bodies was irrelevant to the assembly of photoactive protochlorophyllide. This work reveals a new view on the formation of prolamellar bodies and provides new clues about the function of POR in the etioplast–chloroplast transition. 相似文献
98.
Neurotrophins, which are structurally related to nerve growth factor, have been shown to promote survival of various neurons.
Recently, we found a novel activity of a neurotrophin in the brain: Brain-derived neurotrophic factor (BDNF) enhances expression
of various neuropeptides. The neuropeptide differentiation activity was then compared among neurotrophins both in vivo and
in vitro. In cultured neocortical neurons, BDNF and neurotrophin-5 (NT-5) remarkably increased levels of neuropeptide Y and
somatostatin, and neurotrophin-3 (NT-3) also increased these peptides but required higher concentrations. At elevating substance
P, however, NT-3 was as potent as BDNF. In contrast, NGF had negligible or no effect. Neurotrophins administered into neonatal
brain exhibited slightly different potencies for increasing these neuropeptides: The most marked increase in neuropeptide
Y levels was obtained in the neocortex by NT-5, whereas in the striatum and hippocampus by BDNF, although all three neurotrophins
increased somatostatin similarly in all the brain regions examined. Overall spatial patterns of the neuropeptide induction
were similar among the neurotrophins. Neurons in adult rat brain can also react with the neurotrophins and alter neuropeptide
expression in a slightly different fashion. Excitatory neuronal activity and hormones are known to change expression of neurotrophins.
Therefore, neurotrophins, neuronal activity, and hormones influence each other and all regulate neurotransmitter/peptide expression
in developing and mature brain. Physiological implication of the neurotransmitter/peptide differentiation activities is also
discussed. 相似文献
99.
100.
Arriesgado Dan M. Kurokochi Hiroyuki Nakajima Yuichi Matsuki Yu Uy Wilfredo H. Fortes Miguel D. Campos Wilfredo L. Nadaoka Kazuo Lian Chunlan 《Aquatic Ecology》2015,49(3):357-372
Aquatic Ecology - Persistence of populations at their distributional ranges relies on local population dynamics and the fitness of species with low dispersal potential. We analyzed the population... 相似文献