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81.
Summary A study of enzymatic equipment of enterochromaffine cells (e.c.) in jenual biopsies obtained with a Crosby capsule in normal humans and patients with nontropical sprue was undertaken. The following enzymes were demonstrated: alkaline phosphatase and adenosine triphosphatase (cell membrane), acid phosphatase (corpuscular), non-specific esterase (diffuse and corpuscular, predominantly eserine resistant, in corpuscular localization E 600 resistant), DPN- and TPN-diaphorases and dehydrogenases of lactic acid, malic acid, isocitric acid, glucoso-6-phosphoric acid, succinic acid, -hydroxybutyric acid and -glycerophosphoric acid. Enzyme activities were not equal in all cells suggesting some type of secretory cycle. In most patients with untreated nontropical sprue or with the disease in relapse e.c. were more numerous and hypertrophic with elevated activities of non-specific esterase and acid phosphatase. Implications of these results are briefly discussed.With 8 Figures in the Text, of which 2 in Colour 相似文献
82.
Ve snaze identifikovat fyziologické pochody rozhodující o selektivní toxicitě pyrazonu (1-fenyl-4-amino-5-chorpyridazon-6) jsme sledovali jeho vliv na hlavní enzymové systémy ?ídící dýchání ko?en?. Zatímco jsme u ko?en? zeSinapis alba zjistili úplnou inaktivaci dehydrogenázy kyseliny jantarové—u ko?en? cukrovky je stejný enzym stimulován. 相似文献
83.
A simple biochemical procedure was obtained for studying metabolism ofCorynebacterium diphtheriae during submerged cultivation based on the modification of the assay of dehydrogenase activity using 2,3,5-triphenyltetrazolium
chloride as redox indicator. Results obtained by the estimation of the dehydrogenase activity using TTC are in a good accordance
with oxygen consumption assayed manometrically. By following dehydrogenase activity in submerged cultivations of a production
strain ofCorynebacterium diphtheriae PW8-Weissensee we found that a massive toxin production is connected with the decrease of the activity of cells. This fall
of activity occurs yet during the exponential phase of growth. Especially a sudden fall of succindehydrogenase activity exactly
indicates the beginning of a considerable toxin accumulation in the medium. The presence of inhibitory concentrations of iron
ions in the medium not only increases the level of dehydrogenase activity but changes its whole kinetics. A retarded and irregular
fall of the activity occurs instead of a sharp one typical for good toxin production. 相似文献
84.
Changes in the radioresistance of bacteria after the inhibition of proteosynthesis in the preirradiation phase 总被引:2,自引:1,他引:1
The strain ofEscherichia coli WP2 (tryv) was irradiated with UV light, at a dosage of 240 erg/mm. Proteosynthesis was inhibited by the elimination of the essential
amino acid from the cultivation medium. Changes in radioresistance were followed during 45 minutes of starvation and during
the subsequent 45 minutes of restitution after the addition of the essential amino acid. The radioresistance of the cells
showed a linear increase immediately after the removal of the essential amino acids, proportional to the duration of the inhibition
of proteosynthesis. The increase in radioresistance was shown to be reversible. After the addition of the essential amino
acid there was an immediate decrease in radioresistance which was most marked in the first 15 minutes. 相似文献
85.
Ludmila Šilhánková 《Folia microbiologica》1966,11(1):29-38
Visible region of an absorption spectrum was followed in cells of original strains and of rough mutants ofSaccharomyces cerevisiae andS. cerevisiae var.ellipsoideus. It was found that there are no substantial differences in relative content of cytochromesb andc in aerobically grown rough and smooth yeast forms, in spite of the fact that both forms differ substantially in the metabolic
oxygen quotient. If the cytochromes present were not reduced in washed cells by dithionite or by substrate addition, the rough
forms exhibited a lower cytochrome b:c ratio than the smooth forms. Under anaerobic conditions of cultivation, the rough forms retained a typical aerobic spectrum,
lacking, however, the cytochromea and a3 band; the ratio of cytochromesb andc was changed in favour of cytochromeb (from the original 1.7: 1 up to 3.4: 1). The inability of the rough mutants to produce anaerobic cytochrome spectrum represented
by cytochrome b1 was connected with their inability to reproduce under anaerobic conditions. 相似文献
86.
Šesták Zdeněk 《Biologia Plantarum》1964,6(2):132-141
Quantitative determination of chlorophyll a and β can be made by paper chromatography of acetone extracts of plant material with colorimetric measurement of the eluates from the separated zones. From the suitable solvent systems which give adequate separation of the pigments at a distance of 20 cm. from the start,Hager's mixture (1955) separates the chlorophylls better than the toluene-isopropanol (400: 1 v/v.) mixture, which, however, is better for the separation of carotenoids. Twice the amount of chlorophyll is separated on Whatman 31 ET paper, equally well and with the same time of development, as on Whatman No. 3 paper, on which it is possible to separate a maximum of about 15 μg of chlorophyll pigments per 1 em. start length. Losses on elution are, however, higher on using Whatman 31 ET paper. In plants with a high chlorophyllase activity, the error of determining chlorophyll a andb is greatly reduced if the leaves are placed for 1 min. in boiling water before extraction. For elution of chlorophylla andb from paper it is better to use anhydrous acetone, for chlorophyllides 80% acetone. A comparison of the procedure investigated with the method of two-wave length spectrophotometric measurement of crude acetone extracts showed that in view of the average 10% loss, the chromatographic method is hardly suitable for determining the absolute amounts of chlorophylla andb, although the relation (a/b) can be determined with similar precision by both methods. Moreover, in view of the greater amount of work involved the chromatographic method can only be recommended for confirming the results of spectrophotometrie determination. Quantitative determination of chlorophylls from the area of the spot or from the "RF" value can only be of an informative character. 相似文献
87.
An investigation was made of the anatomical structure of the shoot apex ofSenecio vulgaris L. a photoperiodically neutral plant, and compared with the formation of successive leaf primordia along the axis up to the initiation of the terminal inflorescence. In the shoot apex of a germinating plant a central zone can first be distinguished from the peripheral zone which is composed of small and intensely stained cells. Later, a rib meristem appears. At the time of the initiation of the middle (the largest) leaves, the shoot apex has a distinct small central zone and a well developed peripheral zone and rib meristem. Between these zones there is a group of cells dividing in all directions, the subcentral zone. At the time of initiation of the last leaves, the central zone extends to the flanks and gradually ceases to be distinguishable. At the same time, the subcentral zone increases in size. This is caused first by cell division and later, with the initiation of the last, most reduced leaves, by enlargement of the cells. Vacuolization in the inner part of the apex and the arrangement of the superficial cells in rows parallel to the surface of the apex, is a preparatory step to the initiation of the inflorescence. 相似文献
88.
89.
90.
Biologia Plantarum - The author has studied the relation of the catalase activity in thermal blue-green algae to temperature. Experimental data were taken from the first phase of the reaction, so... 相似文献