Enzyme hydrolysis of cassava peels: treatment by amylolytic and cellulolytic enzymes

Cassava peels provide a cheap non-food biomass waste that can be hydrolyzed to simple sugars as a useful feedstock. Unlike most crop wastes, they have high starch content as well as lignocellulose. In this study, an enzymatic treatment of cassava peels by various concentrations of amylase and glucoamylase is considered. Steam explosion pre-treatments reduced rate and yield of hydrolysis. Milled peels suspended at 10% w/v yielded a maximum reducing sugar of 0.41?g (as glucose) per gram of peels. HPLC analysis showed that levels of soluble oligosaccharides remained low throughout. A pretreatment with amylase at 95?°C slightly increased rates although final yield was the same. Additional treatment with cellulolytic enzymes increases the total hydrolysis yield to 0.61?g (as glucose) per gram of peels representing 91% of the carbohydrate in cassava peels.     

Evaluation of chlorophyll fluorescence in different densities of spring barley

Over the period 2008–2009, field experiments were carried out at the Lithuanian Institute of Agriculture on an Endocalcari-Epihypogleyic Cambisol (CMg-p-w-can). The study was aimed to establish the effects of spring barley crop stand density on the chlorophyll fluorescence and to estimate the relationship between grain yield and chlorophyll fluorescence value. The tests involved three spring barley varieties—Aura DS, Barke and Gustav. The three different stand densities were formed with seed rates of two, four and six million viable seeds per hectare. Our research findings indicated that increasing spring barley stand density did not exert any negative influence on major photosynthetic processes and did not result in any significant qualitative changes in light harvesting system. A significant varietal (factor B) influence was established on minimum fluorescence (F _o) and maximum fluorescence (F _m) values after short dark adaptation indicators (F _fact. = 19.66** and F _fact. = 9.33**, respectively). Growth stage (factor C) significantly influenced all fluorescence indicators—Fo, Fm and quantum efficiency of PSII after short dark adaptation (F _v/F _m)—in the five cases of the six tested. A significant effect of variety and growth stage interaction (B × C) was determined for 66.7% of the tested cases. The article presents a correlation between the grain yield of spring barley and fluorescence parameters. In most cases, the correlation was strong and significant. The interaction between the fluorescence parameters for individual varieties was responsible for 53.8–76.2% of grain yield data variation, which averaged over all varieties, amounted to 21.9–46.1%.     

Degradation of naphthalene by thermophilic bacteria <Emphasis Type="Italic">via</Emphasis> a pathway,through protocatechuic acid

A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene, phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected. Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria.     

Lawful Sinners: Reproductive Governance and Moral Agency Around Abortion in Mexico

The Catholic Hierarchy unequivocally bans abortion, defining it as a mortal sin. In Mexico City, where the Catholic Church wields considerable political and popular power, abortion was recently decriminalized in a historic vote. Of the roughly 170,000 abortions that have been carried out in Mexico City's new public sector abortion program to date, more than 60% were among self-reported Catholic women. Drawing on eighteen months of fieldwork, including interviews with 34 Catholic patients, this article examines how Catholic women in Mexico City grapple with abortion decisions that contravene Church teachings in the context of recent abortion reform. Catholic women consistently leveraged the local cultural, economic, and legal context to morally justify their abortion decisions against church condemnation. I argue that Catholic women seeking abortion resist religious injunctions on their reproductive behavior by articulating and asserting their own moral agency grounded in the contextual dimensions of their lives. My analysis informs conversations in medical anthropology on moral decision-making around reproduction and on local dynamics of resistance to reproductive governance. Moreover, my findings speak to the deficiencies of a feminist vision focused narrowly on fertility limitation, versus an expanded framework of reproductive justice that considers as well the need for conditions of income equality and structural supports to facilitate reproduction and parenting among women who desire to keep their pregnancies.     

Einfluss der Azidität auf Wachstum und Giftempfindlichkeit des PilzesAbsidia orchidis

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