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991.
Comprehensive Computational Analysis of GWAS Loci Identifies CCR2 as a Candidate Gene for Celiac Disease Pathogenesis
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![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
992.
Haitham A. Yacoub Omar A. Al-Maghrabi Ekram S. Ahmed 《Journal of biomolecular structure & dynamics》2017,35(4):836-856
NK-lysins are antimicrobial peptides (AMPs) that participate in the innate immune response and also have several pivotal roles in various biological processes. Such multifunctionality is commonly found among intrinsically disordered proteins. However, NK-lysins have never been systematically analyzed for intrinsic disorder. To fill this gap, the amino acid sequences of NK-lysins from various species were collected from UniProt and used for the comprehensive computational analysis to evaluate the propensity of these proteins for intrinsic disorder and to investigate the potential roles of disordered regions in NK-lysin functions. We analyzed abundance and peculiarities of intrinsic disorder distribution in all-known NK-lysins and showed that many NK-lysins are expected to have substantial levels of intrinsic disorder. Curiously, high level of intrinsic disorder was also found even in two proteins with known 3D-strucutres (NK-lysin from pig and human granulysin). Many of the identified disordered regions can be involved in protein–protein interactions. In fact, NK-lysins are shown to contain three to eight molecular recognition features; i.e. short structure-prone segments which are located within the long disordered regions and have a potential to undergo a disorder-to-order transition upon binding to a partner. Furthermore, these disordered regions are expected to have several sites of various posttranslational modifications. Our study shows that NK-lysins, which are AMPs with a set of prominent roles in the innate immune response, are expected to abundantly possess intrinsically disordered regions that might be related to multifunctionality of these proteins in the signal transduction pathways controlling the host response to pathogenic agents. 相似文献
993.
Omar García‐Castañeda Ramón Gaxiola‐Robles Shane Kanatous Tania Zenteno‐Savín 《Marine Mammal Science》2017,33(3):738-747
An important low molecular weight antioxidant in biological systems is glutathione; its efficiency depends on the equilibrium between its reduced (GSH) and oxidized (GSSG) forms. The oxidized:total glutathione (GSSG:GSH‐Eq) ratio can be used as an indicator of oxidative stress. Previous studies suggest that marine mammals, unlike terrestrial mammals, do not show adverse effects in tissues exposed to ischemia/reperfusion during the peripheral vasoconstriction associated with breath‐hold diving. This is due, in part, to higher antioxidant enzyme activities in marine mammals compared with terrestrial mammals. The objective of this study was to compare circulating glutathione levels among mammals with different diving capacities. Circulating GSH‐Eq, GSH, and GSSG concentrations in erythrocyte samples from northern elephant seals (Mirounga angustirostris), bottlenose dolphins (Tursiops truncatus), neotropical otters (Lontra longicaudis annectens), domestic pigs (Sus scrofa), and humans were quantified using spectrophotometry. Higher GSH‐Eq and GSH concentrations and a lower GSSG:GSH‐Eq index were found in erythrocytes from northern elephant seals and bottlenose dolphins as compared to otters, domestic pigs, and humans. Results suggest that marine mammals, independent of their diving capacity, possess a highly developed antioxidant system, including GSH; continuous availability of GSH could allow these species to avoid oxidative damage and tolerate ischemia/reperfusion and hypoxia/reoxygenation events associated with diving. 相似文献
994.
Phylogeny and taxonomy of the genus Ilyodon Eigenmann, 1907 (Teleostei: Goodeidae), based on mitochondrial and nuclear DNA sequences
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Rosa Gabriela Beltrán‐López Omar Domínguez‐Domínguez José Antonio Guerrero Diushi Keri Corona‐Santiago Humberto Mejía‐Mojica Ignacio Doadrio 《Journal of Zoological Systematics and Evolutionary Research》2017,55(4):340-355
Taxonomy of the live‐bearing fish of the genus Ilyodon Eigenmann, 1907 (Goodeidae), in Mexico, is controversial, with morphology and mitochondrial genetic analyses in disagreement about the number of valid species. The present study accumulated a comprehensive DNA sequences dataset of 98 individuals of all Ilyodon species and mitochondrial and nuclear loci to reconstruct the evolutionary history of the genus. Phylogenetic inference produced five clades, one with two sub‐clades, and one clade including three recognized species. Genetic distances in mitochondrial genes (cytb: 0.5%–2.1%; coxI: 0.5%–1.1% and d‐loop: 2.3%–10.2%) were relatively high among main clades, while, as expected, nuclear genes showed low variation (0.0%–0.2%), with geographic concordance and few shared haplotypes among river basins. High genetic structure was observed among clades and within basins. Our genetic analyses, applying the priority principle, suggest the recognition only of Ilyodon whitei and Ilyodon furcidens, with I. cortesae relegated to an invalid species, the populations of which belong to I. whitei. 相似文献
995.
Mutual independence of alkaline‐ and calcium‐mediated signalling in Aspergillus fumigatus refutes the existence of a conserved druggable signalling nexus
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996.
The anticancer drug doxorubicin causes testicular toxicity as an undesirable effect. The present study was undertaken to investigate the possible protection of ellagic acid and rosmarinic acid during doxorubicin administration. For this purpose eight groups of male Sprague–Dawley rats were used (n = 10), one group received vehicle served as control, and other groups received 5 mg/kg doxorubicin twice a week for 2 weeks for a cumulative dose of 20 mg/kg, ellagic acid (10 mg/kg/day, 14 consecutive days p.o.), rosmarinic acid (75 mg/kg/day, 14 consecutive days p.o.), ellagic acid and rosmarinic acid. The latter three regimens were given to control and doxorubicin‐received rats. Doxorubicin decreased testicular relative weight, sperm count, motility, serum testosterone, testicular glycogen, and sialic acid with increased incidence of histopathological changes, oxidative stress, tumor necrosis factor‐alpha, as well as cholinesterase activity. Conversely, ellagic and rosmarinic acid treatment ameliorated such damage, thus showing the possibility to use as an adjuvant during doxorubicin treatment. 相似文献
997.
Specificity traits consistent with legume‐rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization
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María Eugenia Salas Mauricio Javier Lozano José Luis López Walter Omar Draghi Javier Serrania Gonzalo Arturo Torres Tejerizo Francisco Javier Albicoro Juliet Fernanda Nilsson Mariano Pistorio María Florencia Del Papa Gustavo Parisi Anke Becker Antonio Lagares 《Environmental microbiology》2017,19(9):3423-3438
998.
Rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus Verticillium dahliae 总被引:1,自引:0,他引:1
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Parthasarathy Santhanam Jordi C. Boshoven Omar Salas Kyle Bowler Md Tohidul Islam Mojtaba Keykha Saber Grardy C. M. van den Berg Maor Bar‐Peled Bart P. H. J. Thomma 《Molecular Plant Pathology》2017,18(3):347-362
The initial interaction of a pathogenic fungus with its host is complex and involves numerous metabolic pathways and regulatory proteins. Considerable attention has been devoted to proteins that play a crucial role in these interactions, with an emphasis on so‐called effector molecules that are secreted by the invading microbe to establish the symbiosis. However, the contribution of other types of molecules, such as glycans, is less well appreciated. Here, we present a random genetic screen that enabled us to identify 58 novel candidate genes that are involved in the pathogenic potential of the fungal pathogen Verticillium dahliae, which causes vascular wilt diseases in over 200 dicotyledonous plant species, including economically important crops. One of the candidate genes that was identified concerns a putative biosynthetic gene involved in nucleotide sugar precursor formation, as it encodes a putative nucleotide‐rhamnose synthase/epimerase‐reductase (NRS/ER). This enzyme has homology to bacterial enzymes involved in the biosynthesis of the nucleotide sugar deoxy‐thymidine diphosphate (dTDP)‐rhamnose, a precursor of L‐rhamnose, which has been shown to be required for virulence in several human pathogenic bacteria. Rhamnose is known to be a minor cell wall glycan in fungi and has therefore not been suspected as a crucial molecule in fungal–host interactions. Nevertheless, our study shows that deletion of the VdNRS/ER gene from the V. dahliae genome results in complete loss of pathogenicity on tomato and Nicotiana benthamiana plants, whereas vegetative growth and sporulation are not affected. We demonstrate that VdNRS/ER is a functional enzyme in the biosynthesis of uridine diphosphate (UDP)‐rhamnose, and further analysis has revealed that VdNRS/ER deletion strains are impaired in the colonization of tomato roots. Collectively, our results demonstrate that rhamnose, although only a minor cell wall component, is essential for the pathogenicity of V. dahliae. 相似文献
999.
Adsorption of Zinc Ions by Scenedesmus Obliquus and S. Quadricauda and its Effect on Growth and Metabolism 总被引:3,自引:0,他引:3
H.H. Omar 《Biologia Plantarum》2002,45(2):261-266
Zinc adsorption by two green algae, Scenedesmus obliquus and Scenedesmus quadricauda, was investigated. The maximum adsorption capability of zinc ion obtained from the Langmuir adsorption isotherms was higher for S. obliquus (6.67) than for S. quadricauda (5.03), and S. obliquus was more tolerant to zinc phytotoxicity than S. quadricauda. Lower concentrations of zinc increased dry mass, chlorophylls a and b, carotenoids, and total amino acid contents in both algae. On the other hand, higher concentrations of zinc were inhibitory for growth and the other metabolic activities in a concentration dependent manner. 相似文献