Scanning electron microscopic examination of Thiobacillus ferrooxidans on different support matrix materials in packed bed and fluidized bed bioreactors

Summary Thiobacillus ferrooxidans was used in fixed-film bioreactors to oxidize ferrous iron to ferric iron. The test support matrix materials included activated carbon particles, glass beads, and ion-exchange resin particles. The experimental systems included a fluidized bed approach, which was evaluated with activated carbon only, and a packed bed approach which was tested with each of the support matrix materials. The colonization of the matrix surface was examined with scanning electron microscopy. There were contrasting differences in the bacterial colonization and accumulation of Fe(III) precipitates on the matrix surface among the test materials. The packed bed activated carbon bioreactor displayed the fastest kinetics and the highest amount of cell sorption as well as the roughest and most porous matrix surface.     

Overexpression of VEGF in Testis and Epididymis Causes Infertility in Transgenic Mice: Evidence for Nonendothelial Targets for VEGF

Vascular endothelial growth factor (VEGF) is a key regulator of endothelial growth and permeability. However, VEGF may also target nonendothelial cells, as VEGF receptors and responsiveness have been detected for example in monocytes, and high concentrations of VEGF have been reported in human semen. In this work we present evidence that overexpression of VEGF in the testis and epididymis of transgenic mice under the mouse mammary tumor virus (MMTV) LTR promoter causes infertility. The testes of the transgenic mice exhibited spermatogenic arrest and increased capillary density. The ductus epididymidis was dilated, containing areas of epithelial hyperplasia. The number of subepithelial capillaries in the epididymis was also increased and these vessels were highly permeable as judged by the detection of extravasated fibrinogen products. Intriguingly, the expression of VEGF receptor-1 (VEGFR-1) was detected in certain spermatogenic cells in addition to vascular endothelium, and both VEGFR-1 and VEGFR-2 were also found in the Leydig cells of the testis. The infertility of the MMTV-VEGF male mice could thus result from VEGF acting on both endothelial and nonendothelial cells of the male genital tract. Taken together, these findings suggest that the VEGF transgene has nonendothelial target cells in the testis and that VEGF may regulate male fertility.     

HIGH ENCYSTMENT SUCCESS OF THE DINOFLAGELLATE SCRIPPSIELLA CF. LACHRYMOSA IN CULTURE EXPERIMENTS 1

Close to 100% encystment efficiency and a yield above 10⁵ cysts·mL ^{? 1} were routinely achieved in full strength f/2 medium‐based batch cultures (883 μM NO₃ ^? and 36 μM PO₄ ^{? 3}) of the marine dinoflagellate Scrippsiella cf. lachrymosa Lewis. Increases in cell density led to nutrient depletion in this enriched medium, which was the most likely cause for initiation of cyst formation. Lowering the concentration of either nutrient to 1/10 the initial levels decreased the encystment efficiency, whereas use of ammonium as the N source resulted in both low cell yield and low encystment efficiency. The mandatory dormancy period was ca. 60 days and was not affected by cold dark storage of the cysts. Cysts produced in the initial phase of sexual reproduction were relatively large (length 47 μm, width 31 μm) with a heavy calcareous cover. Cysts produced thereafter lacked apparent calcareous cover and were smaller (length 29 μm, width 19 μm). The decrease of cyst volume (by a factor of 0.24–0.4) suggested strong resource limitation during the course of encystment. However, after the mandatory dormancy period, germination success of the smaller cysts was higher (80%), compared with the larger cysts that had been produced initially (50%). Germling survival (74%) was independent of cyst type but was enhanced by higher nutrient concentration during incubation. The ratio of initial nutrient concentration in the medium to the cyst yield was used as a proxy to estimate the cellular nutrient quota. The conservative estimates of 9 pmol N·cyst ^{? 1} and 0.4 pmol P·cyst ^{? 1} obtained in this manner are at the low end of the range of previous published estimates for other dinoflagellate cysts. Given the high encystment observed in laboratory experiments, we have no reason to assume an inherently lower encystment success in dinoflagellate field populations. Our results do not challenge the low nutrient paradigm for dinoflagellate sexuality. We believe that the high encystment success and cyst yield of this particular species is at least partly due to its ability to achieve very high cell densities in cultures, which evidently leads to nutrient depletion even in f/2 medium.     

The level of autoantibodies against oxidized LDL is not associated with the presence of coronary heart disease or diabetic kidney disease in patients with non-insulin-dependent diabetes mellitus

Oxidation of low-density lipoprotein (LDL) may be an important factor in the development of diabetic macrovascular and renal complications. The level of autoantibodies against oxidized LDL (oxLDL-Ab) can be used as an index of LDL oxidation in vivo. The purpose of this study was to investigate the association between the level of oxLDL-Ab and the presence of coronary heart disease and renal dysfunction in patients with non-insulin-dependent diabetes mellitus (NIDDM). We determined the plasma levels of oxLDL-Ab in 46 NIDDM patients and 48 well matched non-diabetic control subjects. NIDDM patients had a moderately higher level of oxLDL-Ab than control subjects (0.083 ± 0.051 vs. 0.062 ± 0.045, p = 0.04). However, there was no difference in the level of oxLDL-Ab between subjects with and without coronary heart disease, and the level of oxLDL-Ab was not associated with indices of glomerular filtration rate or urinary albumin excretion.     

Interactive effects of nitrogen and phosphorus on the acclimation potential of foliage photosynthetic properties of cork oak,Quercus suber,to elevated atmospheric CO2 concentrations

Leaf gas-exchange and chemical composition were investigated in seedlings of Quercus suber L. grown for 21 months either at elevated (700 μmol mol^–1) or normal (350 μmol mol^–1) ambient atmospheric CO₂ concentrations, [CO₂], in a sandy nutrient-poor soil with either ‘high’ N (0.3 mol N m^–3 in the irrigation solution) or with ‘low’ N (0.05 mol N m^–3) and with a constant suboptimal concentration of the other macro- and micronutrients. Although elevated [CO₂] yielded the greatest total plant biomass in ‘high’ nitrogen treatment, it resulted in lower leaf nutrient concentrations in all cases, independent of the nutrient addition regime, and in greater nonstructural carbohydrate concentrations. By contrast, nitrogen treatment did not affect foliar N concentrations, but resulted in lower phosphorus concentrations, suggesting that under lower N, P use-efficiency in foliar biomass production was lower. Phosphorus deficiency was evident in all treatments, as photosynthesis became CO₂ insensitive at intercellular CO₂ concentrations larger than ≈ 300 μmol mol^–1, and net assimilation rates measured at an ambient [CO₂] of 350 μmol mol^–1 or at 700 μmol mol^–1 were not significantly different. Moreover, there was a positive correlation of foliar P with maximum Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) carboxylase activity (V_cmax), which potentially limits photosynthesis at low [CO₂], and the capacities of photosynthetic electron transport (J_max) and phosphate utilization (P_max), which are potentially limiting at high [CO₂]. None of these potential limits was correlated with foliar nitrogen concentration, indicating that photosynthetic N use-efficiency was directly dependent on foliar P availability. Though the tendencies were towards lower capacities of potential limitations of photosynthesis in high [CO₂] grown specimens, the effects were statistically insignificant, because of (i) large within-treatment variability related to foliar P, and (ii) small decreases in P/N ratio with increasing [CO₂], resulting in balanced changes in other foliar compounds potentially limiting carbon acquisition. The results of the current study indicate that under P-deficiency, the down-regulation of excess biochemical capacities proceeds in a similar manner in leaves grown under normal and elevated [CO₂], and also that foliar P/N ratios for optimum photosynthesis are likely to increase with increasing growth CO₂ concentrations. Symbols: A, net assimilation rate (μmol m^–2 s^–1); A_max, light-saturated A (μmol m^–2 s^–1); α, initial quantum yield at saturating [CO₂] and for an incident Q (mol mol^–1); [CO₂], atmospheric CO₂ concentration (μmol mol^–1); C_i, intercellular CO₂ concentration (μmol mol^–1); C_a, CO₂ concentration in the gas-exchange cuvette (μmol mol^–1); F_B, fraction of leaf N in ‘photoenergetics’; F_L, fraction of leaf N in light harvesting; F_R, fraction of leaf N in Rubisco; Γ*, CO₂ compensation concentration in the absence of R_d (μmol mol^–1); J_max*, capacity for photosynthetic electron transport; J_mc, capacity for photosynthetic electron transport per unit cytochrome f (mol e^–[mol cyt f]^–1 s^–1); K_c, Michaelis-Menten constant for carboxylation (μmol mol^–1); K_o, Michaelis-Menten constant for oxygenation (mmol mol^–1); M_A, leaf dry mass per area (g m^–2); O, intercellular oxygen concentration (mmol mol^–1); [P_i], concentration of inorganic phosphate (mM); P_max*, capacity for phosphate utilization; Q, photosynthetically active quantum flux density (μmol m^–2 s^–1); R_d*, day respiration (CO₂ evolution from nonphotorespiratory processes continuing in the light); Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase; RUBP, ribulose-1,5-bisphosphate; T_l, leaf temperature (°C); U_TPU*, rate of triose phosphate utilization; V_cmax*, maximum Rubisco carboxylase activity; V_cr, specific activity of Rubisco (μmol CO₂[g Rubisco]^–1 s^–1] *given in either μmol m^–2 s^–1 or in μmol g^–1 s^–1 as described in the text.     

Computer analysis of living cells: movements of the chromatoid body in early spermatids compared with its ultrastructure in snap-frozen preparations

 Quantitative analyses of cytoplasmic and nuclear organelle movements in living interphase cells at defined stages of differentiation are few. By phase contrast videomicroscopy and digital imaging techniques, we have traced the path of the chromatoid body (CB) and analysed its rapidly changing positions in relation to the nuclear envelope, Golgi complex and nuclear pale chromatin areas in living early spermatids of the rat. The CB had intimate interactions with the nuclear envelope and moved both in parallel and perpendicular fashion in relation to it. It had successive short contacts with the Golgi complex and nuclear pale chromatin areas. It was also seen to scan between two pale chromatin areas and it had pinocytosis-like transient engulfments during interactions with the pale chromatin. In ultrastructural analysis of snap-frozen preparations, the CB had a large contact area with the nuclear envelope with several intermediate organelles that may be involved in nucleocytoplasmic material transport. It is evident that quantitative image analysis of living cells is a powerful guide for ultrastructural analyses. The snap-freezing technique gives new possibilities for studies of structures that are sensitive to conventional fixation procedures. Accepted: 23 January 1997     

Long-term population changes of the most abundant south Finnish forest birds during the past 50 years

Summary 1. Population indices based on bird censuses (especially line transects) are given for south Finnish forest birds (40 species). The indices refer to 1926–27, 1936, 1936–49, 1952–63 and 1973–77.2. Population increases were observed to be much more frequent than decreases. The main reason for the changes is presumably that the structure of the Finnish forests has changed considerably in recent decades. Many bird populations are thus probably well adapted to habitat changes caused by forestry. However, certain species need habitats destroyed by modern forestry (e. g. old forests); in consequence, it is necessary to maintain sufficient old forests areas for conservational purposes.

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Zusammenfassung 1. Für die 40 häufigsten Waldvögel Südfinnlands werden, meist auf der Grundlage von Linientaxierungen, Populationsindices ermittelt. Diese Indices beziehen sich auf die Jahre 1926/27, 1936, 1936–49, 1952–63 und 1973.2. 22 Arten (Gruppe A der Tabelle) haben eindeutig zugenommen. Die Gründe hierfür sind wohl in Veränderungen der Struktur finnischer Wälder zu suchen, nämlich Zunahme der Fläche von Jungbeständen, der Ausdehnung der Strauchschicht in Altbeständen, der Bedeutung der Fichte als Waldbestandteil und schließlich der Waldränder.3. 7 Arten (Gruppe B) nahmen einheitlich zu mit Ausnahme im SW-Teil des Landes, wo die Zunahme sehr gering war. Diese Arten scheinen in gleicher Weise von den Strukturänderungen finnischer Wälder beeinflußt worden zu sein wie die in Gruppe A. Doch wiesen diese 7 Arten schon 1926/27 im SW Finnlands relativ hohe Dichten auf. Da alle Arten dieser Gruppe hauptsächlich im Süden verbreitet sind, bedeutet die Zunahme in Finnland, daß offenbar die meisten Arten dieser Gruppe ihr Areal nach Norden ausdehnten, was schließlich zu einem deutlichen Populationsanstieg im Untersuchungsgebiet führte.4. 7 Arten (Gruppe C) zeigten keine deutlich erkennbaren Trends. Die Erklärung hierfür scheint in hohen jährlichen Fluktuationen der Populationsgröße als auch in möglichen interspezifischen Konkurrenzverhältnissen zu suchen zu sein.5. 4 Arten (Gruppe D) nahmen deutlich ab, wahrscheinlich in erster Linie infolge der Abnahme des Flächenanteils alter Waldbestände oder der zunehmenden Schwierigkeiten, alte Bäume zu finden.6. So wurden also Zunahmetendenzen weit häufiger als Abnahmetendenzen festgestellt. Diese Ergebnisse stimmen mit anderen Untersuchungen von langfristigen Bestandstrends nordeuropäischer Vogelarten überein. Sie weisen ferner daraufhin, daß offenbar viele Vogelpopulationen an die Habiate, die durch moderne Forstwirtschaft entstehen, gut angepaßt sind. Jedoch sollten diese Ergebnisse vom Standpunkt des Naturschutzes aus nicht falsch interpretiert werden. Sinn des Naturschutzes ist nicht, die Populationsgröße sehr häufiger Arten positiv zu beeinflussen, sondern die Abnahme der Populationsgrößen bedrohter Arten zu verhindern. In diesem Zusammenhang sei auf die Notwendigkeit des Schutzes genügend großer Flächen alter Waldbestände hingewiesen, da eine Reihe von Arten davon im höchsten Maße davon abhängig sind.

     

Infants with univentricular heart have reduced heart rate and blood pressure responses to side motion and altered responses to head-up tilt.

Cardiovascular control was studied in infants with univentricular heart (UVH). Side motion tests and 45-s 45 degrees head-up tilt tests were performed in 11 control and 9 UVH infants at the age of 13 +/- 3.2 wk. In addition, heart rate (HR) reactions to spontaneous arousals and HR variability during slow-wave sleep (SWS) were determined. All UVH infants had been hypoxic for several weeks, and during the sleep study the mean arterial oxyhemoglobin saturation was 82 +/- 5%. Tests were done at night during SWS, confirmed by polysomnographic recording. Continuous beat-to-beat blood pressure (BP) was measured. In the side-motion tests, control infants consistently showed a transient increase in HR and BP. This response was markedly reduced in all of the UVH infants (P < 0.0001). In tilt tests, the UVH infants showed normal BP responses, but, although a sustained 2.0% decrease in HR was observed in the controls, the UVH infants presented with a sustained 2.6% mean HR increase (P = 0.005). The UVH infants also showed attenuated HR acceleration during spontaneous arousals (P = 0.01), but HR variability did not differ significantly from the controls. In conclusion, UVH infants with chronic hypoxia exhibit defective vestibulosympathetic pathways, as expressed by an absence of acute HR and BP reactivity to side motion. HR reactions to postural challenge and spontaneous arousal are also altered. Autonomic function abnormalities in these infants are suggested to be secondary to hypoxia.