全文获取类型
收费全文 | 321篇 |
免费 | 29篇 |
专业分类
350篇 |
出版年
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 8篇 |
2018年 | 9篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 16篇 |
2014年 | 14篇 |
2013年 | 21篇 |
2012年 | 29篇 |
2011年 | 23篇 |
2010年 | 15篇 |
2009年 | 12篇 |
2008年 | 8篇 |
2007年 | 13篇 |
2006年 | 14篇 |
2005年 | 7篇 |
2004年 | 15篇 |
2003年 | 7篇 |
2002年 | 11篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 9篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1983年 | 4篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1976年 | 4篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1967年 | 3篇 |
1966年 | 2篇 |
1965年 | 3篇 |
排序方式: 共有350条查询结果,搜索用时 15 毫秒
341.
Optimized conditions for the fermentation of Helicobacter pylori and production of vacuolating cytotoxin 总被引:2,自引:0,他引:2
Antonio Marchini Paola Massari Roberto Manetti Roberto Olivieri 《FEMS microbiology letters》1994,124(1):55-59
Abstract We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvement in the production of vacuolating cytotoxin. 相似文献
342.
343.
Mitotic delay and repair in human lymphocytes 总被引:4,自引:0,他引:4
344.
S. Francesca L. Vitale C. Arena G. Raimondi F. Olivieri V. Cirillo A. Paradiso M. C. de Pinto A. Maggio A. Barone M. M. Rigano 《Plant biology (Stuttgart, Germany)》2022,24(1):62-74
- Climate change is increasing the frequency of high temperature shocks and water shortages, pointing to the need to develop novel tolerant varieties and to understand the mechanisms employed to withstand combined abiotic stresses.
- Two tomato genotypes, a heat-tolerant Solanum lycopersicum accession (LA3120) and a novel genotype (E42), previously selected as a stable yielding genotype under high temperatures, were exposed to single and combined water and heat stress. Plant functional traits, pollen viability and physiological (leaf gas exchange and chlorophyll a fluorescence emission measurements) and biochemical (antioxidant content and antioxidant enzyme activity) measurements were carried out. A Reduced Representation Sequencing approach allowed exploration of the genetic variability of both genotypes to identify candidate genes that could regulate stress responses.
- Both abiotic stresses had a severe impact on plant growth parameters and on the reproductive phase of development. Growth parameters and leaf gas exchange measurements revealed that the two genotypes used different physiological strategies to overcome individual and combined stresses, with E42 having a more efficient capacity to utilize the limiting water resources. Activation of antioxidant defence mechanisms seemed to be critical for both genotypes to counteract combined abiotic stresses. Candidate genes were identified that could explain the different physiological responses to stress observed in E42 compared with LA3120.
- Results here obtained have shown how new tomato genetic resources can be a valuable source of traits for adaptation to combined abiotic stresses and should be used in breeding programmes to improve stress tolerance in commercial varieties.
345.
346.
347.
348.
349.
350.
Cyclodextrins for growth ofHelicobacter pylori and production of vacuolating cytotoxin 总被引:3,自引:0,他引:3
Antonio Marchini Maria d’Apolito Paola Massari Marco Atzeni Michael Copass Roberto Olivieri 《Archives of microbiology》1995,164(4):290-293
Growth ofHelicobacter pylori in liquid culture requires the addition of media supplements that often interfere with subsequent purification of bacterial
antigens. In order to determine whether cyclodextrins can substitute for conventionalH. pylori growth supplements, we culturedH. pylori in the presence of five commercially available cyclodextrins. The effect of these compounds on the production of the vacuolating
cytotoxin antigen was evaluated. Several cyclodextrins supported flourishing growth and permitted the consistent production
of vacuolating cytotoxin. These data suggest that Brucella broth supplemented with cyclodextrins is an improved medium for
bacterial culture and industrial production ofH. pylori antigens. 相似文献