全文获取类型
收费全文 | 6727篇 |
免费 | 604篇 |
国内免费 | 3篇 |
专业分类
7334篇 |
出版年
2023年 | 37篇 |
2022年 | 74篇 |
2021年 | 160篇 |
2020年 | 72篇 |
2019年 | 123篇 |
2018年 | 139篇 |
2017年 | 113篇 |
2016年 | 198篇 |
2015年 | 341篇 |
2014年 | 439篇 |
2013年 | 535篇 |
2012年 | 578篇 |
2011年 | 573篇 |
2010年 | 366篇 |
2009年 | 341篇 |
2008年 | 445篇 |
2007年 | 428篇 |
2006年 | 416篇 |
2005年 | 386篇 |
2004年 | 341篇 |
2003年 | 317篇 |
2002年 | 307篇 |
2001年 | 45篇 |
2000年 | 51篇 |
1999年 | 73篇 |
1998年 | 63篇 |
1997年 | 42篇 |
1996年 | 35篇 |
1995年 | 36篇 |
1994年 | 30篇 |
1993年 | 28篇 |
1992年 | 21篇 |
1991年 | 15篇 |
1990年 | 15篇 |
1989年 | 17篇 |
1988年 | 10篇 |
1987年 | 19篇 |
1986年 | 16篇 |
1985年 | 10篇 |
1983年 | 12篇 |
1981年 | 3篇 |
1980年 | 13篇 |
1979年 | 7篇 |
1978年 | 8篇 |
1977年 | 9篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1965年 | 2篇 |
排序方式: 共有7334条查询结果,搜索用时 15 毫秒
91.
Romain Guyot Marion de la Mare Véronique Viader Perla Hamon Olivier Coriton José Bustamante-Porras Valérie Poncet Claudine Campa Serge Hamon Alexandre de Kochko 《BMC plant biology》2009,9(1):22
Background
Coffea canephora, also called Robusta, belongs to the Rubiaceae, the fourth largest angiosperm family. This diploid species (2x = 2n = 22) has a fairly small genome size of ≈ 690 Mb and despite its extreme economic importance, particularly for developing countries, knowledge on the genome composition, structure and evolution remain very limited. Here, we report the 160 kb of the first C. canephora Bacterial Artificial Chromosome (BAC) clone ever sequenced and its fine analysis. 相似文献92.
The increasing ability to generate large-scale, quantitative proteomic data has brought with it the challenge of analyzing such data to discover the sequence elements that underlie systems-level protein behavior. Here we show that short, linear protein motifs can be efficiently recovered from proteome-scale datasets such as sub-cellular localization, molecular function, half-life, and protein abundance data using an information theoretic approach. Using this approach, we have identified many known protein motifs, such as phosphorylation sites and localization signals, and discovered a large number of candidate elements. We estimate that ~80% of these are novel predictions in that they do not match a known motif in both sequence and biological context, suggesting that post-translational regulation of protein behavior is still largely unexplored. These predicted motifs, many of which display preferential association with specific biological pathways and non-random positioning in the linear protein sequence, provide focused hypotheses for experimental validation. 相似文献
93.
94.
Said El?Shamieh Marion Neuillé Angélique Terray Elise Orhan Christel Condroyer Vanessa Démontant Christelle Michiels Aline Antonio Fiona Boyard Marie-Elise Lancelot Mélanie Letexier Jean-Paul Saraiva Thierry Léveillard Saddek Mohand-Sa?d Olivier Goureau José-Alain Sahel Christina Zeitz Isabelle Audo 《American journal of human genetics》2014,94(4):625-633
95.
Irene Pila-Castellanos Diana Molino Joe McKellar Laetitia Lines Juliane Da Graca Marine Tauziet Laurent Chanteloup Ivan Mikaelian Laurne Meyniel-Schicklin Patrice Codogno Jacky Vonderscher Cdric Delevoye Olivier Moncorg Eric Meldrum Caroline Goujon Etienne Morel Benoit de Chassey 《PLoS pathogens》2021,17(2)
Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza virus entry, host cells experience modifications of endomembranes, including those used for virus trafficking and replication. Here we report that influenza virus infection modifies mitochondrial morphodynamics by promoting mitochondria elongation and altering endoplasmic reticulum-mitochondria tethering in host cells. Expression of the viral RNA recapitulates these modifications inside cells. Virus induced mitochondria hyper-elongation was promoted by fission associated protein DRP1 relocalization to the cytosol, enhancing a pro-fusion status. We show that altering mitochondrial hyper-fusion with Mito-C, a novel pro-fission compound, not only restores mitochondrial morphodynamics and endoplasmic reticulum-mitochondria contact sites but also dramatically reduces influenza replication. Finally, we demonstrate that the observed Mito-C antiviral property is directly connected with the innate immunity signaling RIG-I complex at mitochondria. Our data highlight the importance of a functional interchange between mitochondrial morphodynamics and innate immunity machineries in the context of influenza viral infection. 相似文献
96.
Xiaoyu Luo Hugo Mouquet Olivier Schwartz Warner C. Greene 《The Journal of biological chemistry》2021,297(4)
The progressive loss of CD4+ T cells during HIV infection of lymphoid tissues involves both the apoptotic death of activated and productively infected CD4 T cells and the pyroptotic death of large numbers of resting and abortively infected bystander CD4 T cells. HIV spreads both through cellular release of virions and cell-to-cell transmission involving the formation of virological synapses. Cell-to-cell transmission results in high-level transfer of large quantities of virions to the target cell exceeding that achieved with cell-free virions. Broadly neutralizing anti-HIV antibodies (bNAbs) binding to HIV envelope protein capably block cell-free virus spread, and when added at higher concentrations can also interdict cell-to-cell transmission. Exploiting these distinct dose–response differences, we now show that four different bNAbs block the pyroptotic death of bystander cells, but only when added at concentrations sufficient to block cell-to-cell transmission. These findings further support the conclusion that HIV killing of abortively infected bystander CD4 T cells requires cell-to-cell transfer of virions. As bNAbs attract more interest as potential therapeutics, it will be important to consider the higher concentrations of these antibodies required to block the inflammatory death of bystander CD4 T cells. 相似文献
97.
Routaboul JM Dubos C Beck G Marquis C Bidzinski P Loudet O Lepiniec L 《Journal of experimental botany》2012,63(10):3749-3764
Little is known about the range and the genetic bases of naturally occurring variation for flavonoids. Using Arabidopsis thaliana seed as a model, the flavonoid content of 41 accessions and two recombinant inbred line (RIL) sets derived from divergent accessions (Cvi-0×Col-0 and Bay-0×Shahdara) were analysed. These accessions and RILs showed mainly quantitative rather than qualitative changes. To dissect the genetic architecture underlying these differences, a quantitative trait locus (QTL) analysis was performed on the two segregating populations. Twenty-two flavonoid QTLs were detected that accounted for 11-64% of the observed trait variations, only one QTL being common to both RIL sets. Sixteen of these QTLs were confirmed and coarsely mapped using heterogeneous inbred families (HIFs). Three genes, namely TRANSPARENT TESTA (TT)7, TT15, and MYB12, were proposed to underlie their variations since the corresponding mutants and QTLs displayed similar specific flavonoid changes. Interestingly, most loci did not co-localize with any gene known to be involved in flavonoid metabolism. This latter result shows that novel functions have yet to be characterized and paves the way for their isolation. 相似文献
98.
Olivier Maurin Artemis Anest Sidonie Bellot Edward Biffin Grace Brewer Tristan Charles-Dominique Robyn S. Cowan Steven Dodsworth Niroshini Epitawalage Berta Gallego Augusto Giaretta Renato Goldenberg Deise J.P. Gonçalves Shirley Graham Peter Hoch Fiorella Mazine Yee Wen Low Catherine McGinnie Fabián A. Michelangeli Sarah Morris Darin S. Penneys Oscar Alejandro Pérez Escobar Yohan Pillon Lisa Pokorny Gustavo Shimizu Vanessa G. Staggemeier Andrew H. Thornhill Kyle W. Tomlinson Ian M. Turner Thais Vasconcelos Peter G. Wilson Alexandre R. Zuntini William J. Baker Félix Forest Eve Lucas 《American journal of botany》2021,108(7):1087-1111
99.
Development and validation of a new dynamic computer‐controlled model of the human stomach and small intestine 下载免费PDF全文