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101.
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J M Oliver R D Berlin 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1982,299(1095):215-235
The long-range movements of membrane ligand-receptor complexes into surface caps and into the pseudopods of cells performing phagocytosis, the uropods of motile cells and the cleavage furrows of dividing cells appear to be analogous processes. A common mechanism to explain these movements must take into account several recent observations. First, laser photobleaching studies have indicated that Concanavalin A-receptor movement occurs unidirectionally; and analyses of Con A redistribution by quantitative video intensification microscopy (QUAVIM) have shown that movement may exceed the maximum rates measured for protein diffusion in membranes. These are the results predicted for a process of directed migration but not for a process of diffusion with entrapment. In addition it has been found that membrane receptors may segregate out of as well as into cap, pseudopod, uropod and cleavage furrow regions and that topographical heterogeneity on asymmetric cells is not restricted to membrane molecular determinants but extends to a range of endocytic functions and to a macromolecular complex, the coated pit. All dynamic surface events are arrested during mitosis. A new model for the regulation of plasma membrane topography has been developed from these diverse quantitative, functional and morphological data. Its essence is the entrainment of selected membrane determinants on membrane waves directed towards regions such as caps, pseudopods, uropods and cleavage furrows. The waves are initiated by tension due to asymmetric microfilament-membrane interaction. 相似文献
104.
105.
Uptake of Exogenous Glutamate and Aspartate by Circumventricular Organs but Not Other Regions of Brain 总被引:1,自引:1,他引:0
Madelon T. Price John W. Olney Oliver H. Lowry Susan Buchsbaum 《Journal of neurochemistry》1981,36(5):1774-1780
Abstract: Glutamate (Glu) and aspartate (Asp) concentrations in blood and selected regions of brain were measured at sequential intervals over a 3-h period following subcutaneous administration of Glu, Asp, or Glu plus Asp (2 mg/g body wt) to 4-day-old mouse or rat pups. Marked serum elevations of the administered amino acids (peak values exceeding 200 times control levels) were detected within 1 h. In circumventricular organ (CVO) regions of brain, which are thought to have no blood-brain barriers, a sharp and steady increase in tissue concentrations of the administered amino acids (peak values 4–10 times higher than control levels) occurred during a 15–120 min interval, whereas no appreciable increases were detected in other brain regions. When 2 mg/g Glu plus 2 mg/g Asp were administered, CVO tissue concentrations of each amino acid rose to approximately the same level obtained when the individual amino acids were given. It is concluded that blood-brain barriers preventing net entry of Glu or Asp into brain proper are relatively well established by the 4th postnatal day in rodents, but that CVO brain regions lack such barriers; selective access of blood-borne Glu or Asp to CVO neurons explains why these neurons are selectively destroyed by systemic administration of these neurotoxic amino acids. 相似文献
106.
Complete accounts of the natural products chemistry of Bonnemaisonia nootkana, B. asparagoides, B. hamifera and Trailliella intricata are described. In contrast to the chemistry of the closely related alga Asparagopsis, Bonnemaisonia spp. do not produce halomethanes, but instead an array of C7-C9 halogen-containing ketones, alcohols and carboxylic acids. Biomimetic syntheses of these compounds suggest they are precursors and products of in vivo Favorsky rearrangements. 相似文献
107.
Cytogenetic and electrophoretic analyses on 2n = 28 strains ofAsphodelus cerasiferus strongly suggest that the basic number x = 14 of the genusAsphodelus is of secondary polyploid origin from x = 7. 相似文献
108.
Constance Oliver 《In vitro cellular & developmental biology. Plant》1980,16(4):297-305
Summary Methods have been developed for isolating and maintaining differentiated rat exorbital lacrimal, parotid, and pancreatic acinar
cells for up to 1 month in culture. The dissociated cells retained their differentiated morphology when cultured as suspension
cultures at 35°C with the appropriate secretagogue (exorbital lacrimal, 10−6
M carbamyl choline; pancreas 10−5
M carbamyl choline; parotid, 10−6
M isoproterenol). Under these conditions the cells remained viable and differentiated for up to 4 weeks in culture and continued
to incorporate3H-leucine at rates similar to those of freshly isolated cells. If secretagogue was omitted from the medium, the cells rapidly
degenerated. These results indicate that differentiated from the medium, the cells rapidly degenerated. These results indicate
that differentiated exocrine gland acinar cells may be maintained in vitro and utilized as a model system for the study of
secretory processes. 相似文献
109.
Insulin antagonism of glucocorticoid induction of tyrosine aminotransferase in cultured foetal hepatocytes 总被引:7,自引:0,他引:7
Polyadenylated RNA from developing Artemia salina cysts was fractionated by centrifugation through a sucrose gradient containing methylmercuric hydroxide (CH3HgOH). Aliquots of each fraction were directly added to a rabbit reticulocyte lysate to program protein synthesis in vitro. The translation products were assayed for eukaryotic elongation factor Tu (eEF-Tu) by immunoprecipitation with an antibody raised in rabbits and purified by affinity chromatography. The immunoprecipitated radioactivity was analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate. Sequences coding for eEF-Tu sediment in the 20-S region of the gradient and form a major component of the poly(A)-containing RNA. The mRNA of the 20-S region, comprising about 10% of the poly(a)-containing RNA fractionated on the gradient, has been translated in vitro and 30% of the translation products represent immunoprecipitable eEF-Tu protein chains with an Mr of 50000. 相似文献
110.
The marine opisthobranch molluscAplysia punctata was cultured at the Laboratoire de Biologie Marine in Concarneau, France.A. punctata veligers settled and underwent metamorphosis on the algaLomentaria articulata, but not onUlva spp., Palmaria marina, Laminaria spp. andFucus spp.Research supported by grants from The Arts Foundation and the Lerner Fund for Marine Research of the American Museum of Natural History. We wish to thank Director Yves Legal, College de France for his support and cooperation. 相似文献