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Neuza Domingues André R. A. Marques Rita Diogo Almeida Calado Inês S. Ferreira Cristiano Ramos José Ramalho Maria I. L. Soares Telmo Pereira Luís Oliveira José R. Vicente Louise H. Wong Inês C. M. Simões Teresa M. V. D. Pinho e Melo Andrew Peden Cláudia Guimas Almeida Clare E. Futter Rosa Puertollano Winchil L. C. Vaz Otília V. Vieira 《Traffic (Copenhagen, Denmark)》2023,24(7):284-307
13.
Inflorescence-specific genes from Arabidopsis thaliana encoding glycine-rich proteins 总被引:6,自引:0,他引:6
D.E. de Oliveira L.O. Franco C. Simoens J. Seurinck J. Coppieters J. Botterman M. Van Montagu 《The Plant journal : for cell and molecular biology》1993,3(4):495-507
Genomic and cDNA clones for three inflorescence-specific genes from Arabidopsis thaliana were isolated and characterized. The genes are tandemly organized in the genome on a 10 kb fragment. The expression of these genes is coordinately regulated in a developmental and organ-specific pattern. They are expressed predominantly in anthers at the later stage of flower development. The primary structure of the encoded gene products exhibits comparable features consisting of a hydrophobic domain at the N-terminal region followed by repeated glycine-rich motifs. Little homology is observed either between the glycine-rich domain of the three genes or with previously described glycine-rich proteins from other plant species. 相似文献
14.
Coelho-Rocha Nina Dias de Jesus Luís Cláudio Lima Barroso Fernanda Alvarenga Lima da Silva Tales Fernando Ferreira Enio Gonçalves José Eduardo dos Santos Martins Flaviano de Oliveira Carvalho Rodrigo Dias Barh Debmalya Azevedo Vasco Ariston de Carvalho 《Probiotics and antimicrobial proteins》2023,15(1):160-174
Probiotics and Antimicrobial Proteins - Beneficial effects of Lactiplantibacillus plantarum strains have been widely reported. Knowing that the effects of probiotic bacteria are strain-dependent,... 相似文献
15.
J. C. Zanuncio J. L. D. Saavedra H. N. Oliveira D. Degheele P. De Clercq 《Biocontrol Science and Technology》1996,6(4):619-626
The development of the Neotropical predatory pentatomid Brontocoris tabidus on an artificial diet based on beef meat and liver was evaluated. The predator showed significantly longer nymphal development and lower adult weights on this diet than when reared on pupae of the mealworm Tenebrio molitor. The survival of nymphs fed exclusively on the artificial diet was somewhat lower compared with feeding on T. molitor pupae. When B. tabidus was bred on this artificial diet during part of its nymphal period i.e. during the second; second and third; and second, third and fourth instars , and was subsequently returned to T. molitor pupae, the predator nymphs completed the nymphal stage with a developmental rate similar to that of nymphs fed on live prey throughout. The adults attained after switching from the artificial diet to live prey from the third and fourth instar onwards had similar weights to those in the control. Considering the relatively good results obtained with B. tabidus and other members of the pentatomid subfamily Asopinae, this meat-based diet may be a valuable alternative for use in the mass production of predatory pentatomids. 相似文献
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The aquatic fungus Blastocladiella emersonii provides a system for studying the regulation of expression of regulatory (R) and catalytic (C) subunits of cAMP-dependent protein kinase (PKA). Blastocladiella cells contain a single PKA with properties very similar to type II kinases of mammalian tissues. During development cAMP-dependent protein kinase activity and its associated cAMP-binding activity change drastically. We have previously shown that the increase in cAMP-binding activity during sporulation is due to de novo synthesis of R subunit and to an increase in the translatable mRNA coding for R (Marques et al., Eur. J. Biochem. 178, 803, 1989). In the present work we have continued these studies to investigate the mechanism by which the changes in the level of kinase activity take place. The C subunit of Blastocladiella has been purified; antiserum has been raised against it and used to determine amounts of C subunit throughout the fungus' life cycle. A sharp increase in C subunit content occurs during sporulation and peaks at the zoospore stage. Northern blot analyses, using Blastocladiella C and R cDNA probes, have shown that the levels of C and R mRNAs parallel their intracellular protein concentrations. These results indicate a coordinate pretranslational control for C and R subunit expression during differentiation in Blastocladiella. 相似文献
18.
H B Nader M A Porcionatto I L Tersariol M A Pinhal F W Oliveira C T Moraes C P Dietrich 《The Journal of biological chemistry》1990,265(28):16807-16813
The purification of two heparitinases and a heparinase, in high yields from Flavobacterium heparinum was achieved by a combination of molecular sieving and cation-exchange chromatography. Heparinase acts upon N-sulfated glucosaminido-L-iduronic acid linkages of heparin. Substitution of N-sulfate by N-acetyl groups renders the heparin molecule resistant to degradation by the enzyme. Heparitinase I acts on N-acetylated or N-sulfated glucosaminido-glucuronic acid linkages of the heparan sulfate. Sulfate groups at the 6-position of the glucosamine moiety of the heparan sulfate chains seem to be impeditive for heparitinase I action. Heparitinase II acts upon heparan sulfate producing disulfated, N-sulfated and N-acetylated-6-sulfated disaccharides, and small amounts of N-acetylated disaccharide. These and other results suggest that heparitinase II acts preferentially upon N,6-sulfated glucosaminido-glucuronic acid linkages. The total degradation of heparan sulfate is only achieved by the combined action of both heparitinases. The 13C NMR spectra of the disaccharides formed from heparan sulfate and a heparin oligosaccharide formed by the action of the heparitinases are in accordance to the proposed mode of action of the enzymes. Comparative studies of the enzymes with the commercially available heparinase and heparitinase are described. 相似文献
19.
Dulce E. Oliveira Ana Lúcia C. Santos Neto Anita D. Panek 《Analytical biochemistry》1981,113(1):188-192
A quantitative in situ assay of yeast α-glucosidase involving permeabilization of the cells by freezing and thawing is described. The assay was applied to different strains in different physiological states and was shown to give results comparable to those obtained with total cell homogenates. The primary advantage of the in situ assay was the possibility of analyzing a large number of samples from the same culture during a growth curve using a very reduced cell mass. 相似文献
20.