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441.
Ana Flávia do Vale Neves Machado Cleonice Maria Cardoso Lobato Rafael Rodrigues Gusmão Luciano Fogaça de Assis Montag Bruno Silveira Prudente 《Zeitschrift fur angewandte Ichthyologie》2020,36(5):745-747
The length-weight relationship is important for the study of populational dynamics. This study aimed to describe the length-weight relationships (LWR) of 11 fish species sampled on 18 streams of the Capim river basin on eastern Amazon. Specimens were captured in 2014, 2015 and 2017, using sieve nets with 2 mm of mesh size. Fixed specimens were fixed in 10% formalin and conserved in alcohol 70%, measured for standard length (SL, 0.1 cm) and total weight (W, 0.01 g). The 11 species analysed were: Hemigrammus bellottii, Hemigrammus ocellifer, Hyphessobrycon heterorhabdus, Iguanodectes rachovii, Copella arnoldi, Nannostomus trifasciatus, Apistogramma regani, Apistogramma agassizii, Gymnorhamphichthys rondoni, Hypopygus lepturus, Anablepsoides urophthalmus. 相似文献
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Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus)
443.
Caroline Andrade Rocha Tania Mary Cestari Hugo Alberto Vidotti Gerson Francisco de Assis Gustavo Pompermaier Garlet Rumio Taga 《Journal of molecular histology》2014,45(4):447-461
This study aimed to evaluate morphometrically the bone formation and immunohistochemically the expression of vascular endothelial growth factor (VEGF) and metalloproteinase (MMP)-2 and -9 during the healing of critical-size defects treated with sintered anorganic bone (sAB). The 8-mm diameter full-thickness trephine defects created in the parietal bones of rats were filled with sAB (test group) or blood clot (CSD-control group). At 7, 14, 21, 30, 90 and 180 days postoperatively (n = 6/period) the volume of newly formed bone and total number of immunolabeled cells (Ntm) for each protein were determined. Bone formation was smaller and faster in the CSD-control group, stabilizing at 21 days (6.74 mm3). The peaks of VEGF, MMP-2 and MMP-9 occurred at 7 and 14 days in fibroblasts and osteoblasts, with mean reduction of 0.80 time at 21 days, keeping constant until 180 days. In the test group, sAB provided continuous bone formation between particles throughout all periods. The peak of MMP-2 was observed at 7–14 days in connective tissue cells and for VEGF and MMP-9 at 30 days in osteoblasts and osteocytes. Ntm for VEGF, MMP-2 and MMP-9 were in average, respectively, 3.70, 2.03 and 5.98 times higher than in the control group. At 180 days, newly formed bone (22.9 mm3) was 3.74 times greater in relation to control. The physical and chemical properties of sAB allow increased autocrine expression of VEGF, MMP-2 and MMP-9, favoring bone formation/remodeling with very good healing of cranial defects when compared to natural repair in the CSD-control. 相似文献
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Jordana Grazziela Alves Coelho-dos-Reis Livia Passos Mariana Costa Duarte Marcelo Grossi Araújo Ana Carolina Campi-Azevedo Andréa Teixeira-Carvalho Vanessa Peruhype-Magalh?es Bruno Caetano Trindade Raquel dos Santos Dias Marina Lobato Martins Anna Barbara de Freitas Carneiro-Proietti Ant?nio Carlos Guedes Denise Utsch Gon?alves Olindo Assis Martins-Filho 《PLoS neglected tropical diseases》2013,7(7)
In the present study, the frequency, the activation and the cytokine and chemokine profile of HTLV-1 carriers with or without dermatological lesions were thoroughly described and compared. The results indicated that HTLV-1-infected patients with dermatological lesions have distinct frequency and activation status when compared to asymptomatic carriers. Alterations in the CD4+HLA-DR+, CD8+ T cell, macrophage-like and NKT subsets as well as in the serum chemokines CCL5, CXCL8, CXCL9 and CXCL10 were observed in the HTLV-1-infected group with skin lesions. Additionally, HTLV-1 carriers with dermatological skin lesions showed more frequently high proviral load as compared to asymptomatic carriers. The elevated proviral load in HTLV-1 patients with infectious skin lesions correlated significantly with TNF-α/IL-10 ratio, while the same significant correlation was found for the IL-12/IL-10 ratio and the high proviral load in HTLV-1-infected patients with autoimmune skin lesions. All in all, these results suggest a distinct and unique immunological profile in the peripheral blood of HTLV-1-infected patients with skin disorders, and the different nature of skin lesion observed in these patients may be an outcome of a distinct unbalance of the systemic inflammatory response upon HTLV-1 infection. 相似文献
447.
Isabela Jacob Moro Flávio Alexandre Carvalho Juhan Augusto Scardelato Pereira Gabriela Brasil Romão André Faibicher Winner Duque Rodrigues Renata Pires Assis Iguatemy Lourenço Brunetti Junya de Lacorte Singulani Ana Marisa Fusco-Almeida André Gonzaga dos Santos 《化学与生物多样性》2023,20(8):e202300329
Varronia curassavica displays anti-inflammatory, antiulcerogenic, and antioxidant activities. Herein, we employed new UHPLC –UV green chromatographic methods for the analysis of in vitro antioxidant and anti-inflammatory activities of V. curassavica and its embryotoxicity in Zebrafish. Cordialin A, brickellin, and artemetin were purified from the ethanol (EtOH) extract of V. Curassavica leaves and identified using spectrometric techniques. In line with Green Analytical Chemistry principles, the proposed UHPLC methods involve the use of ethanol as organic modifier with low mobile phase consumption, and without sample pretreatment (OLE-UHPLC-UV). The application of the Agree and HPLC-EAT tools for greenness assessment yielded this pattern: HPLC-UV (reference)<UHPLC-UV<OLE-UHPLC-UV. Zebrafish assay results showed that 70 % EtOH extract of V. Curassavica leaves exhibited lower toxicity compared to 100 % EtOH extract, with LC50 of 164.3 and 122.9 μg/mL, respectively, in 24 h post fertilization. Some embryos exhibited malformation phenotypes in the heart, somites, and eyes, mainly in higher extract concentrations. Extracts and brickellin exhibited higher antioxidant activity in the DPPH⋅ assay, while brickellin+artemetin displayed higher antioxidant activity compared to the extracts and isolated flavones in the O2⋅− and HOCl/OCl− scavenging assays. Cordialin A and brickellin exhibited low COX-1, COX-2, and phospholipase A2 inhibition. 相似文献
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449.
Lisiane G. Londero Débora K. Rieger Fernanda Hansen Simone L. Silveira Tiago L. Martins Francisco Lulhier Roselis S. da Silva Diogo O. Souza Adriano M. de Assis 《Cell biochemistry and function》2013,31(8):636-642
Long‐chain polyunsaturated n‐3 fatty acids (n‐3 LCPUFAs) have hypolipidemic effects and modulate intermediary metabolism to prevent or reverse insulin resistance in a way that is not completely elucidated. Here, effects of these fatty acids on the lipid profile, phosphoenolpyruvate carboxykinase (PEPCK) activity, lipid synthesis from glucose in epididymal adipose tissue (Ep‐AT) and liver were investigated. Male rats were fed a high‐sucrose diet (SU diet), containing either sunflower oil or a mixture of sunflower and fish oil (SU–FO diet), and the control group was fed a standard diet. After 13 weeks, liver, adipose tissue and blood were harvested and analysed. The dietary n‐3 LCPUFAs prevented sucrose‐induced increase in adiposity and serum free fat acids, serum and hepatic triacylglycerol and insulin levels. Furthermore, these n‐3 LCPUFAs decreased lipid synthesis from glucose and increased PEPCK activity in the Ep‐AT of rats fed the SU–FO diet compared to those fed the SU diet, besides reducing lipid synthesis from glucose in hepatic tissue. Thus, the inclusion of n‐3 LCPUFAs in the diet may be beneficial for the prevention or attenuation of dyslipidemia and insulin resistance, and for reducing the risk of related chronic diseases. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
450.
Marcella Rungue Victor Melo David Martins Priscila C. Campos Gabriela Leles Izabela Galvo Viviani Mendes Mariana Aganetti gatha Pedersen Natan R. G. Assis Raiany Santos Geovanni D. Cassali Ana Lúcia B. Godard Flaviano S. Martins Sergio C. Oliveira Anglica T. Vieira 《PLoS neglected tropical diseases》2021,15(2)
Brucella abortus is a Gram-negative bacterium responsible for a worldwide zoonotic infection—Brucellosis, which has been associated with high morbidity rate in humans and severe economic losses in infected livestock. The natural route of infection is through oral and nasal mucosa but the invasion process through host gut mucosa is yet to be understood. Studies have examined the role of NLRP6 (NOD-like receptor family pyrin domain-containing-6 protein) in gut homeostasis and defense against pathogens. Here, we investigated the impact of gut microbiota and NLRP6 in a murine model of Ba oral infection. Nlrp6-/- and wild-type (WT) mice were infected by oral gavage with Ba and tissues samples were collected at different time points. Our results suggest that Ba oral infection leads to significant alterations in gut microbiota. Moreover, Nlrp6-/- mice were more resistant to infection, with decreased CFU in the liver and reduction in gut permeability when compared to the control group. Fecal microbiota transplantation from WT and Nlrp6-/- into germ-free mice reflected the gut permeability phenotype from the donors. Additionally, depletion of gut microbiota by broad-spectrum-antibiotic treatment prevented Ba replication in WT while favoring bacterial growth in Nlrp6-/-. Finally, we observed higher eosinophils in the gut and leukocytes in the blood of infected Nlrp6-/- compared to WT-infected mice, which might be associated to the Nlrp6-/- resistance phenotype. Altogether, these results indicated that gut microbiota composition is the major factor involved in the initial stages of pathogen host replication and partially also by the resistance phenotype observed in Nlrp6 -/- mice regulating host inflammation against Ba infection. 相似文献