Phospholipidgranula im verhornenden Oesophagusepithel

Zusammenfassung Im Epithel des Meerschweinchenoesophagus fanden wir mit Ausnahme des Stratum corneum in jeder Schicht 0,1–0,3 große, lamellär-strukturierte Granula. Die Granula werden meistens von einer Membran (unit-membrane) umgeben, die ein lamelläres System in sich einschließt, das eine Periodizität von 60–70 Å aufweist und aus dunklen und hellen Lamellen besteht. Unsere Annahme, daß die Granula Phospholipide enthalten, wird durch die Beobachtung unterstützt, daß die Lokalisation der lichtmikroskopisch durchgeführten Baker-Reaktion vollkommen mit der Verteilung der lamellären Granula im elektronenmikroskopischen Bild übereinstimmt. Nach Pyridinextraktion ist die Baker-Reaktion negativ, während im elektronenmikroskopischen Material an Stelle der Granula nur Vakuolen zu finden sind.Die Granula erscheinen im Stratum germinativum meistens in Verbindung mit dem Golgiapparat und entleeren sich in Höhe des Stratum granulosum in den interzellulären Raum. Zwischen den Lamellen des Stratum corneum ist das Material der Granula als eine homogene, dunkle, amorphe Deckschicht vorhanden. Ihre Aufgabe besteht wahrscheinlich in der Steigerung der Resistenz der Hornschicht.

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Summary Lamellated granules of 0,1 to 0,3 in diameter are consistently found in all strata of the keratinizing epithelium of the guinea pig oesophagus. The granules are surrounded by a single membrane (unit membrane) and contain a lamellated system consisting of dark and light bands showing a periodicity of 60 to 70 Å. The supposed phospholipid nature of the granules was supported by the positive Baker-reaction at the light microscope level. The distribution of the Baker-positive substance was identical with that of the lamellated granules at the electron micrograph. After extraction with pyridin, the Baker-reaction turned out to be negative while on the electron micrographs the substance of the lamellated granules was lost.The granules first appear near the Golgi region of the stratum germinativum and are emptied into the extra-cellular space at the level of the stratum granulosum. The substance of the granules, after having lost their lamellated structure, remains between the keratinized layers as a homogenous, dense material. Its function probably consists in increasing the resistance of the cornified layer against chemical agents.

     

A combined micro-and semi-micro colorimetric determination of long-chain fatty acids from plant cutin

Summary Re-examination of the colorimetric fatty acid determination with copper nitrate, followed by complex formation with DIECA has shown that the method is not reliable if applied as described by Duncombe (1962, 1963): The Cu concentration is too high, the DIECA concentration much too low and the wavelength chosen (440 m) is suitable only for very low fatty acid concentrations.According to the results reported here the following alterations have to be adopted: The concentration of the copper nitrate solution should be 3%, a 0.5% solution of DIECA in butanol has to be used and measurements should be done at 492 m. The method described here offers the opportunity to determine fatty acid concentrations in the semi-micro range by measuring the filtered chloroform phase directly at 691 m, covering a range between 175 g/ml to 1.2 mg/ml. If the concentration turns out to be lower than 200 g F. A./ml, the same sample can be used for a micro-determination (up to 200 g/ml) at 492 m, after formation of the yellowish-brown complex by addition of 0.1 ml 0.5% butanolic DIECA solution to 1.0 ml of the chloroform phase.The method has been applied to determine the amount of free F. A. in cutin layers and cutin powder, revealing that the latter contains 5.6 times more free F. A. than the intact material. The free F. A. within the polymer seem to serve as interconnections for the main units of the cutin polylipid.     

Amylase heterogeneity variants in man

The existence of six amylase heterogeneity variants in serum and urine has been discovered in a group of 111 families with twins, and the frequence of each variant was determined. It had been shown that each of these amylase heterogeneity variants is inherited codominantly, and the mode of inheritance is demonstrated schematically.These amylase heterogeneity variants may be used as an aid to twin zygosity determination and also in paternity problems.     

Die Wirkungsweise von Cercosporella herpotricboides Fron, dem Erreger der Halmbruchkrankheit des Getreides

In halt: 1. Einleitung und Aufgabenstellung.—2. Art und Ausmaß der Scnäden im Feld-versuch.—3. Wirkungen des Krankheitserregers auf Sommergetreide.—4. Bekämpfungsmöglichkeiten.—5. Besprechung der Ergebnisse.—Zusammenfassung.—Summary. —Literaturverzeichnis.     

Some factors stimulating and inhibiting pancreatic deoxyribonuclease

Для выяснения роли ДН-азы в реакции трансформации исследовали действие различных сред, применяемых при трансформации пневмококков, на активность панкреатической ДН-азы. Эти виды среды резко повышали активность фермента. Далее, было установлено, что это повышение вызывается yeast-экстрактом, входящим в состав этих сред. С помщяю спектрального анализа и пламенного спектрофотометра в yeast-экстракте были обнаружены Mg²⁺, Ca²⁺, K⁺ и Na⁺. Изучалось действие этих ионов на активность ДН-азы и было установлено, что в присутствии Mg²⁺ (5×10^?3 m)иCa²⁺(вконцентрации 2×10^?4 m) резко повышается активность фермента, тогда как прибавление к этой системе KCl в концентрации 2×10^?1, 2×10^?2, 2×10^?3 и 2×10^?4 m оказывает угнетающее действие.—Обсуждается возможное влияние этих ионов на peaкцию трансформации.     

Metabolites ofStreptomyces noursei

Было установлено, что актиномицет Str. noursei образует амиды некоторых разветвленных алифатических кислот: изобутирамид, метилэтилацетамид, изовалерамид, а, вероятно, и более сложные гомологи, как изокапронамид, изоэнантамид и 4-метилкапронамид. Обсуждается биогенез этих веществ.     

MORPHOLOGICAL AND BIOCHEMICAL CORRELATES OF CEREBRAL MICROSOMES : I. Isolation and Chemical Characterization

Microsomal fractions, both homogeneous in appearance and functionally operative, were isolated from a homogenate of rat cerebral cortex by fractionation in water. The preparations thus obtained contain the membranous elements of the endoplasmic reticulum, synaptic vesicles, and ribosomes. Esterase, ATPase, and glutamine synthetase were found to be present and fully functional in the microsomal fractions isolated in water. The contamination of the water-isolated microsomal fractions by mitochondria and lysosomes was found to be considerably lower than in microsomal pellets isolated in sucrose. The contamination by nerve ending particles, as judged by electron microscopy and by the levels of soluble lactic dehydrogenase entrapped in the cytoplasm of the particles, was also low. Most of the contamination by mitochondria and nerve ending particles could be removed by treatment of the microsomal pellet with 150 mM NaCl. Resistant to elution by this treatment is the lysosomal contamination as well as microsomal esterase and ATPase. Glutamine synthetase, on the other hand, was almost totally solubilized. Microsomal preparations isolated in water are also shown to contain amounts of protein, RNA, phospholipid, and ganglioside comparable to those found in microsomal preparations isolated in sucrose.