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Shanenko E. F. Nikolaev Yu. A. Ganina V. I. Serykh I. N. Oleskin A. V. Mukhamedzhanova T. G. Grigorieva N. V. El’-Registan G. I. 《Microbiology》2022,91(4):378-394
Microbiology - The intestinal microbiota is known to perform an important role in terms of the host organism’s metabolism and life-sustaining activities. One of the main mechanisms of the... 相似文献
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Probiotics and Antimicrobial Proteins - In light of recent data, microorganisms should be construed as organisms that are capable of communication and collective behaviors. Microbial communication... 相似文献
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SE?Aleshin AV?Timofeev MV?Khoretonenko LG?Zakharova GV?Pashvykina JR?Stephenson AM?Shneider AD?AltsteinEmail author 《BMC microbiology》2005,5(1):45
Background
Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immune system in different ways during vaccination procedures. The purpose of the project was to study the ability of recombinant vaccinia virus (VV) and bacterial plasmid, both carrying the NS1 gene from tick-borne encephalitis (TBE) virus under the control of different promoters, to protect mice against lethal challenge using a heterologous prime-boost vaccination protocol. 相似文献25.
This review covers the modern concepts and recent data demonstrating the integrity and coherence of microbial populations (colonies, biofilms, etc.) as peculiar "super-organisms." Special attention is given to such relevant phenomena as apoptosis, bacterial altruism, quorum effects, collective differentiation of microbial cells, and the formation of population-level structures such as an extracellular matrix. Emphasis is placed on the channels in colonies and agents of intercellular communication in microbial populations. The involvement of a large number of evolutionarily conserved communicational facilities and patterns of intercellular interactions is underscored. Much attention is also given to the role of colonial organization and intercellular communication in parasite/commensal/symbiont-multicellular host organism systems. 相似文献
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EG Smirnova GS Muromtsev AV Osipenko EE Khavkin LS Yaguzhinsky 《Biochemistry. Biokhimii?a》1998,63(9):1021-1028
Activation of highly specific biochemical processes by simple chemical agents is demonstrated for morphogenesis (anlage and development of female gametophyte in cereal) and mitosis (in cell cultures and animal and plant tissues). The effects of these agents are tissue-specific. Structure--activity relationship is analyzed in this group of compounds. Thus, the phenomenon reveals the exact pathways of the influence of allelopathic and anthropogenic chemical agents on evolution of plant biocenoses. 相似文献
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OI Klychnikov AV Drabkin OV Vasilenko YS Pavlov MS Trofimova IN Smolenskaya AA Rozenkranz AS Sobolev AV Babakov 《Biochemistry. Biokhimii?a》1998,63(9):1083-1089
Higher plant plasma membranes carry receptors of different affinity for the phytotoxin fusicoccin. Reception of fusicoccin involves proteins belonging to the highly conserved 14-3-3 family, but the complete structure of the fusicoccin receptor (FCR) is unknown. Using radiation inactivation analysis, we estimated the molecular masses of low-affinity and high-affinity FCR at 63 +/- 7 and 130 +/- 15 kD, respectively. The dose dependences of receptor inactivation indicate that microsomal specimens contain "silent" FCRs of 420 +/- 90 kD in amounts commensurate with that of the active FCRs. Both low- and high-affinity FCRs are inactivated by hydrolytic enzymes from the outer surface of the plasma membrane, and impairment of protoplast integrity causes an irreversible transition of the low-affinity binding site into the high-affinity one. A scheme is proposed for the organization of different types of FCR in the plasma membrane, implying that the membrane affinity for fusicoccin reflects the interaction between proteins in the FCR complex. 相似文献
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Vitaly D. Samuilov Gernot Renger Vladimir Z. Paschenko Alexander V. Oleskin Michail V. Gusev Olga N. Gubanova Sergei S. Vasil'ev Eugene L. Barsky 《Photosynthesis research》1995,46(3):455-465
The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,3,4,5,6-pentachlorophenol (PCP) and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole (TTFB) inhibited the Hill reaction with K3[Fe(CN)6] (but not with SiMo) in chloroplast and cyanobacterial membranes (the I50 values were approx. 1–2, 4–6 and 0.04–0.10 M, respectively). The inhibition is due to oxidation of the uncouplers on the Photosystem II donor side (ADRY effect) and their subsequent reduction on the acceptor side, ie. to the formation of a cyclic electron transfer chain around Photosystem II involving the uncouplers as redox carriers. The relative amplitude of nanosecond chlorophyll fluorescence in chloroplasts was increased by DCMU or HQNO and did not change upon addition of uncouplers, DBMIB or DNP-INT; the HQNO effect was not removed by the uncouplers. The uncouplers did not inhibit the electron transfer from reduced TMPD or duroquinol to methylviologen which is driven by Photosystem I. These data show that CCCP, PCP and TTFB oxidized on the Photosystem II donor side are reduced by the membrane pool of plastoquinone (Qp) which is also the electron donor for K3 [Fe(CN)6] in the Hill reaction as deduced from the data obtained in the presence of inhibitors. Inhibition of the Hill reaction by the uncouplers was maximum at the pH values corresponding to the pK of these compounds. It is suggested that the tested uncouplers serve as proton donors, and not merely as electron donors on the oxidizing side of Photosystem II.Abbreviations ADRY-
acceleration of the deactivation reactions of the water-splitting enzyme system Y
- ANT2p-
2-(3-chloro-4-trifluoromethyl) anilino-3,5-dinitrothiophene
- CCCP-
carbonyl cyanide m-chlorophenylhydrazone
- DBMIB-
2,5-dibromo-3-methyl 6-isopropyl-p-benzoquinone
- DCMU-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DNP-INT-
2-iodo-6-isopropyl-3-methyl 2,4,4-trinitrodiphenyl ether
- DPC-
1,5-diphenylcarbazide
- DPIP-
2,6-dichlorophenolindophenol
- FCCP-
carbonyl cyanide p-trifuoromethoxyphenylhydrazone
- FeCy-
potassium ferricyanide
- HQNO-
2-n-heptyl-4-hydroxyquinoline N-oxide
- (MN)4-
the tetranuclear Mn cluster of water oxidizing complex
- P680-
photoactive Chl of the reaction center of Photosystem II
- PCP-
2,3,4,5,6-pentachlorophenol
- PS-
photosystem
- QA and QB-
primary and secondary plastoquinones of PS II
- QC and QZ-
plastoquinone binding sites in the cytochrome blf complex
- Qp-
membrane pool of plastoquinone
- SiMo-
sodium silicomolybdate
- TMPD-
N,N,N-tetramethyl-p-phenylenediamine
- TTFB-
4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole
- WOC-
water oxidixing complex
- YZ-
tyrosine-161 of the Photosystem II D1 polypeptide 相似文献
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AV Bezrukov 《Biotechnic & histochemistry》2017,92(1):29-35
I give an historical account and analysis of the scientific priority of the discovery of the polychrome staining of microscopic biological preparations provided by mixtures of eosin plus methylene blue and its derivatives, especially azure B. I maintain that both the formal priority for the discovery of the polychrome staining phenomenon and credit for initiating the development of a technique of polychrome staining properly belong to D. L. Romanowsky. His scientific work demonstrated the possibility of using a simple technique to stain hematological preparations selectively to give good contrast, high resolution and the ability to identify malaria parasites. Romanowsky’s approach constituted the starting point for the development of a family of polychrome stains for microscopic investigation of hematological preparations by a number of his contemporaries. 相似文献
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