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51.
Effects of non-thermal plasma on mammalian cells 总被引:1,自引:0,他引:1
Kalghatgi S Kelly CM Cerchar E Torabi B Alekseev O Fridman A Friedman G Azizkhan-Clifford J 《PloS one》2011,6(1):e16270
Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, blood coagulation, and cancer treatment, a mechanistic understanding of the interaction of non-thermal plasma with living tissues is required. Using mammalian cells in culture, it is shown here that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellular reactive oxygen species (ROS). We have utilized γ-H2AX to detect DNA damage induced by non-thermal plasma and found that it is initiated by production of active neutral species that most likely induce formation of organic peroxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent, suggesting that plasma treatment may lead to replication arrest or formation of single-stranded DNA breaks; however, plasma does not lead to formation of bulky adducts/thymine dimers. 相似文献
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Hedwig Lerner 《Cell and tissue research》1956,43(6):554-565
Zusammenfassung An der Spitze der Hautzähnchen von Corydoras bildet sich kein Schmelz aus, sondern Durodentin und zwar in sehr unterschiedlichem Umfange und wechselnder Art: bald erscheint es als eine dünnere oder dickere Oberflächenschicht, bald erfaßt es den Spitzenabschnitt als Ganzes, bald tritt es an einzelnen Stellen der Spitze auf. 相似文献
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Fedor N. Novikov Viktor S. Stroylov Oleg V. Stroganov Ghermes G. Chilov 《Journal of molecular modeling》2010,16(7):1223-1230
In the current study an innovative method of structural filtration of docked ligand poses is introduced and applied to improve
the virtual screening results. The structural filter is defined by a protein-specific set of interactions that are a) structurally
conserved in available structures of a particular protein with its bound ligands, and b) that can be viewed as playing the
crucial role in protein-ligand binding. The concept was evaluated on a set of 10 diverse proteins, for which the corresponding
structural filters were developed and applied to the results of virtual screening obtained with the Lead Finder software.
The application of structural filtration resulted in a considerable improvement of the enrichment factor ranging from several
folds to hundreds folds depending on the protein target. It appeared that the structural filtration had effectively repaired
the deficiencies of the scoring functions that used to overestimate decoy binding, resulting into a considerably lower false
positive rate. In addition, the structural filters were also effective in dealing with some deficiencies of the protein structure
models that would lead to false negative predictions otherwise. The ability of structural filtration to recover relatively
small but specifically bound molecules creates promises for the application of this technology in the fragment-based drug
discovery. 相似文献
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